LOCUS       sequence-49112-e        1417 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1417)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MINA
FEATURES             Location/Qualifiers
     source          1..1417
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg cagctggggg gccttcagct ttaaactttg acagtcccag tagtctcttt
      121 gaaagtttaa tctcgcccat caagacagag acttttttca aggaattctg ggagcagaag
      181 ccccttctca ttcagagaga tgaccctgca ctggccacat actatgggtc cctgttcaag
      241 ctaacagatc tgaagagtct gtgcagccgg gggatgtact atggaagaga tgtgaatgtc
      301 tgccggtgtg tcaatgggaa gaagaaggtt ttaaataaag atggcaaagc acactttctt
      361 cagctgagaa aagattttga tcagaaaagg gcaacgattc agtttcacca acctcagaga
      421 tttaaggatg agctttggag gatccaggag aagctggaat gttactttgg ctccttggtt
      481 ggctcgaatg tgtacataac tcccgcagga tctcagggcc tgccgcccca ttatgatgat
      541 gtcgaggttt tcatcctgca gctggaggga gagaaacact ggcgcctcta ccaccccact
      601 gtgcccctgg cacgagagta cagcgtggag gccgaggaaa ggatcggcag gccggtgcat
      661 gagtttatgc tgaagccggg tgatttgttg tactttccca gaggaaccat tcatcaagcg
      721 gacactcctg cggggctggc ccactcgact cacgtgacca tcagcaccta ccagaacaat
      781 tcatggggag atttcctttt ggataccatc tcggggcttg tatttgatac tgcaaaggaa
      841 gacgtggagt tacggaccgg cataccccgg cagctgctcc tgcaggtgga atccacaact
      901 gttgctacaa gacgattaag tggcttcctg aggacacttg cagaccggct ggagggcacc
      961 aaagaactgc tttcctcaga catgaagaag gattttatta tgcacagact ccccccttac
     1021 tctgcgggag atggggcaga gctgtcaaca ccaggtggaa agttaccgag gctggacagt
     1081 gtagtgagac tgcagtttaa agaccacatt gtcctcacag tactgccgga tcaagatcaa
     1141 tctgatgaaa ctcaagaaaa gatggtgtac atctatcatt ccttaaagaa tagtagagag
     1201 acacacatga tgggaaatga ggaggaaaca gagtttcatg gacttcgctt ccctttgtca
     1261 catttggatg cactgaagca aatttggaat agtccagcta tttctgtcaa ggacctgaaa
     1321 cttactacag atgaggaaaa ggaaagcctg gtattatccc tctggacaga atgtttaatt
     1381 caagtagtct agcagtaaag gtggatacgg atccgaa
//
