LOCUS       sequence-49114-e        1018 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1018)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: CBLB
FEATURES             Location/Qualifiers
     source          1..1018
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             947..967
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             968..985
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             986..1009
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cttaagaagg agatatacta tgcaagctgc cgcagatcgc aggaccgtgg agaagacttg
       61 gaagctcatg gacaaagtgg taagactgtg ccaaaatccc aaacttcagt tgaaaaatag
      121 cccaccatat atacttgata ttttgcctga tacatatcag catttacgac ttatattgag
      181 taaatatgat gacaaccaga aacttgccca actcagtgag aatgagtact ttaaaatcta
      241 cattgatagc cttatgaaaa agtcaaaacg ggcaataaga ctctttaaag aaggcaagga
      301 gagaatgtat gaagaacagt cacaggacag acgaaatctc acaaaactgt cccttatctt
      361 cagtcacatg ctggcagaaa tcaaagcaat ctttcccaat ggtcaattcc agggagataa
      421 ctttcgtatc acaaaagcag atgctgctga attctggaga aagttttttg gagacaaaac
      481 tatcgtacca tggaaagtat tcagacagtg ccttcatgag gtccaccaga ttagctctgg
      541 cctggaagca atggctctaa aatcaacaat tgatttaact tgcaatgatt acatttcagt
      601 ttttgaattt gatattttta ccaggctgtt tcagccttgg ggctctattt tgcggaattg
      661 gaatttctta gctgtgacac atccaggtta catggcattt ctcacatatg atgaagttaa
      721 agcacgacta cagaaatata gcaccaaacc cggaagctat attttccggt taagttgcac
      781 tcgattggga cagtgggcca ttggctatgt gactggggat gggaatatct tacagaccat
      841 acctcataac aagcccttat ttcaagccct gattgatggc agcagggaag gattttatct
      901 ttatcctgat gggaggagtt ataatcctga tttaactgga ttagcagaga acctctactt
      961 ccaatcgcac catcatcacc accatgatta caaggatgac gacgataagt gaggatcc
//
