LOCUS       sequence-491144-        6827 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Archael (Asgard group archaeon SRVP18_Atabeyarchaeia-1) 16S rRNA 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6827)
  AUTHORS   Van Beek JM, Robles G, Mewalal R, Blaby I, Hatzenpichler RO
  TITLE     A collection of archaeal 16S rRNA Clone-FISH cultures for probe 
            validation in fluorescence in situ hybridization experiments
  JOURNAL   Microbiol Resour Announc. 2025 October. 0:e01131-25.
REFERENCE   2  (bases 1 to 6827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microbiol 
            Resour Announc. 2025 October. 0:e01131-25."
COMMENT     Sequence Label: Atabey1
FEATURES             Location/Qualifiers
     source          1..6827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      complement(380..427)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(2860..2879)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2861..2879)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2886..2903)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2886..2902)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2895..2917)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     oriT            3144..3253
                     /note="incP origin of transfer"
     rep_origin      3443..3871
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3530..3549)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3740..3761
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             4215..5009
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4269..4288)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4879..4898
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(5027..5095,5096..5887)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5245..5264)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6058..6646
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6547..6566
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6800..6817
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg cttaagccga
      301 attctgcaga tatccatcac actggcggcc gctcgagcat gcatctagac catcacactg
      361 gcggccgctc gagcatgcac aaaaaacccc tcaagacccg tttagaggcc ccaaggggtt
      421 atgctagacg gagtcttacg gctaccttgt tacgacttct ccccccttgc cgagcctggg
      481 ttcgacgtgg ccaaaaaggc tacgtctcac cctaacccga ctcgggtgga gcgacgggcg
      541 gtctacactc gcgctaagcg cttcctagac acgatgtgtg caaggagcag ggacagtatt
      601 caccgcgcga tgatgacgcg cgattactag ggattccatg ttcacgaggg cgagttgcag
      661 ccctcgatcc caactgagat tgggtttcgg gattaccttc ccctttcggg gtcggaaccc
      721 attgtcccaa ccattgcagc ccgcgtgtgg cccgggggat tcggggcata ctgacctgcc
      781 gtggccccct ccttcctcca gcttatcgcc ggcagtccct acagtgtgct cagacccatt
      841 tctaagcctg gtagcaactg taagcagggg tctcgttcgt tgcctgactt gacaggacac
      901 ctcacggcac gaactgacga cggcttcgca gacctacagc catcgtccga actcatccgt
      961 tgtcataatt gccttgactc gtattcctac gagcgcctgc tggagagcag ctttgtttcg
     1021 cggttcacat acaacgatga tatcccaacc cttagtggtt aggtctgcaa gtttgatctg
     1081 gagtcttgaa agataatcgt ggtcgcgaaa gtatccgcta acctcgatta ttgtgtggtt
     1141 gattaggaag tcagggatgt atccatctat ctccggttca taatcgaatt tgtatccatg
     1201 tttgcgcagc aaatccgcaa cccgcagttc caggcggcta cggtattcta ttccatatcc
     1261 atccttgtat cggtatctgg ctgatgaatg tatggcagat ccagagagtt ttgtcagata
     1321 cctgatcccg tacttggctt ttacagcttc accgccagcc ttcgctgctc gcgatagcca
     1381 ctccttatgg gggtggcgtt cgtgcattat tcttgctgct ctcttgcgtg tctctgcgta
     1441 ccattgagtc tctgtgacca ctcgcggcac gtccttgatc tcatatgtct tcttcaacct
     1501 tgcgaacacg ttgagaggca tccggaagca gcctctatac gcccagtctc gaaaggtatt
     1561 ccactcaacc aagtcatcaa cgtgcctgta tactccttgc agagccccac agtcttgttg
     1621 gcgcagacga cggataagac tcttcgtttt gaccagaaca tacttcctct tgtcgtcaac
     1681 gatcgtcatg atcccctcct aagaaaggca caagcgagtt catttgaact ccctcgccaa
     1741 cacttgtcaa cagccttcct acagagacga ctatgagtca catcgtagag aggttagagg
     1801 aagccgcgaa gagttttagg acgacctgaa ggcctcatgc accacctctc agcttgtcaa
     1861 gcaaggtcgt caacctggcc ttcattctgc tgtcgccccc ggtaagtttc ccggcgttga
     1921 gtccaattaa accgcaggct tcaccccttg tggtgctccc ccgccaattc ctttaagttt
     1981 cagccttgcg gccgtactcc ccaggcggcg ggcttaacgg cttcccttcg gcactggagc
     2041 agcacgtggc cgctccaaca cctagcccgc atagtttaca gctaggacta cccgggtatc
     2101 taatccggtt cgctccccta gctttcgtcc ctcaccgtcg ggcgcgctcc agctgaacgc
     2161 cttcgccaca ggtggtccta ctgggattat aggatttcac ccctacccca gtagtaccct
     2221 cagcctctct cgcccccaag ccaagcagta tcctcagcac gccgtcagtt aggctgacgg
     2281 atttagccaa ggacttacaa ggccggctac ggacgcttta ggcccaataa acatcccaac
     2341 cactcgagga gctggtttta ccgcggcggc tggcaccagc cttgccctcc ccttattccc
     2401 acagcttttt atactgtgga aaagccgccc ttagcggacg gcactcggag tagccccgtc
     2461 gctgtttcca gcattgcgga ggtttcgcgc ctgctgcgcc ccgtaggacc tggggccttg
     2521 tctcagtccc catctccggg ctaccgctct cacggcccgt accgattgtc ggtttggtga
     2581 gccgttaccc caccaacagc ctaatcggcc gcggtcccat cctctggcga cgttggcaaa
     2641 catggtgtca aagccgtttg ggtgaaaagc cattccagaa ctattcacct atcgggtatt
     2701 agctccagtt tcccggaggt atccccgtcc ggaggttagg ttaaccacgt gttactgagc
     2761 cgtacgccgc gcctcgccat agtccgaggc gctcgactag catggcttag tcccactccg
     2821 atagcggttg ggtccggcag gatcaaccgg tttaattcgc cctatagtga gtcgtattac
     2881 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     2941 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     3001 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggacgcgctg gaaggcagta
     3061 caccttgata ggtgggctgc ccttcctggt tggcttggtt tcatcagcca tccgcttgcc
     3121 ctcatctgtt acgccggcgg tagccggcca gcctcgcaga gcaggattcc cgttgagcac
     3181 cgccaggtgc gaataaggga cagtgaagaa ggaacacccg ctcgcgggtg ggcctacttc
     3241 acctatcctg cccggctgac gccgttggat acaccaagga aagtctacac gaaccctttg
     3301 gcaaaatcct gtatatcgtg cgaaaaagga tggatatacc gaaaaaatcg ctataatgac
     3361 cccgaagcag ggttatgcag cggaaaagcg ctgcttccct gctgttttgt ggaatatcta
     3421 ccgactggaa acaggcaaat gcaggaaccc tgtagcggcg cattaagcgc ggcgggtgtg
     3481 gtggttacgc gcagcgtgac cgctacactt gctagcgccc tagcgcccgc tcctttcgct
     3541 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     3601 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     3661 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     3721 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     3781 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     3841 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa ttcagggcgc aagggctgct
     3901 aaaggaagcg gaacacgtag aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg
     3961 tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag caggtagctt
     4021 gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca agcgaaccgg
     4081 aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta aactggatgg
     4141 ctttcttgcc gccaaggatc tgatggcgca ggggatcaag atctgatcaa gagacaggat
     4201 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     4261 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     4321 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     4381 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     4441 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     4501 aagtgccggg gcaggatctc ctgtcatccc accttgctcc tgccgagaaa gtatccatca
     4561 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     4621 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     4681 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     4741 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     4801 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     4861 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     4921 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     4981 tctatcgcct tcttgacgag ttcttctgaa ttgaaaaagg aagagtatga gtattcaaca
     5041 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     5101 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     5161 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     5221 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     5281 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     5341 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     5401 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     5461 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     5521 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     5581 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     5641 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     5701 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     5761 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     5821 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     5881 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     5941 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     6001 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6061 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6121 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6181 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     6241 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6301 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6361 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6421 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6481 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6541 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     6601 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     6661 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     6721 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     6781 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaag
//
