LOCUS       sequence-491171-        5945 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Archael (Heimdallarchaeota AB_125) 16S rRNA expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5945)
  AUTHORS   Van Beek JM, Robles G, Mewalal R, Blaby I, Hatzenpichler RO
  TITLE     A collection of archaeal 16S rRNA Clone-FISH cultures for probe 
            validation in fluorescence in situ hybridization experiments
  JOURNAL   Microbiol Resour Announc. 2025 October. 0:e01131-25.
REFERENCE   2  (bases 1 to 5945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microbiol 
            Resour Announc. 2025 October. 0:e01131-25."
COMMENT     Sequence Label: HeiAB125
FEATURES             Location/Qualifiers
     source          1..5945
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      complement(380..427)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(1978..1997)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1979..1997)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2004..2021)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2004..2020)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2013..2035)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     oriT            2262..2371
                     /note="incP origin of transfer"
     rep_origin      2561..2989
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2648..2667)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2858..2879
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             3333..4127
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3387..3406)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3997..4016
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(4145..4213,4214..5005)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4363..4382)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5176..5764
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5665..5684
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5918..5935
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg cttaagccga
      301 attctgcaga tatccatcac actggcggcc gctcgagcat gcatctagac catcacactg
      361 gcggccgctc gagcatgcac aaaaaacccc tcaagacccg tttagaggcc ccaaggggtt
      421 atgctagagg aggtgatcca cttgcgatac ataagcctcg acttatgtat ttccgcaggt
      481 tccccttacc gaaaagggag actgcctctc accaatttta cggatacctt gttacgactt
      541 ttgcatcgtc acgaggccca gttttgactg aaccgattcg cctcagcctc aaccgaacct
      601 catttcgttg caacgacggg cggtgtgtac aaaaagcggg gacgtattct ccgtgcgatg
      661 atgacacacg gatactaggc gctccatgtt cacgaggtcg gttccagacc tcgatcccta
      721 ctgcgaacag gtttggaggt ccgcttctcc tttcggagtc gcttctcatt gtcctgtcca
      781 ttgtgatgcg tgtgtagccc tggggatttg gagcatactg acctgccgtg gcccgctctt
      841 tcatctatct tcatcgatag cgatccccct attgtcctca gccaagccga agcttgctct
      901 tagcaaatag gggcgcgggt ctcgctcgtt tactcactta agagcacacc tcacggcacg
      961 agcttgagac ggccatgcac tacctctctg ttttattcgt gtaaggtctt cagcctgaca
     1021 cacatgtaac agtcgcccca ggtgagtttc ctcgcgttga gtcgaattaa accacacatc
     1081 ccgtgcctgg tggcgctttc ccgtcaattc atttaagttt cagccttgcg accgtactcc
     1141 ccaggcggag gatttaatag tttccctttg gcactgacca ggcccttagc catgaccaac
     1201 acctaatcct catcgtttac ggccaggact acaggggtct ctaatccctt tcgctcccct
     1261 ggctttcatc catcaccgtc ggagatattc cagtgaaccg ccttcgccac tgtgggtcct
     1321 gcgagaatca acgcatttca ccgcttctct cgcagtaccg ttcacctctc ctattcccta
     1381 ggttagcggt atctctgaca gcctacaagt taagcttgca gatttaatca gagacctact
     1441 tacccggcta cggatgcttt aagcccaata atcgtcctag gcactcgcag agctggtttt
     1501 accgcggcgg ctggcaccag ccttgccctc tgcttgctgt tggagctacg aacactccat
     1561 cacagccaag gttctccttg gcactcagat tcgcggtctc acgctttcgc gcattgggca
     1621 caattcctaa ctgctgcaca ccgtagtgct aggaccagtg tctaagtgtc cttctcgggg
     1681 cgctaactct cattacccct accgatcatc gcctaagtga tcctttacat cacttactag
     1741 ctaatcggcc gcggcctcat cctatggcga accgaagccc ctttgggtat tggctccttc
     1801 cagaattcca tacctatcgg gtattaatct cagtttcccg aggttatccc cgtccatagg
     1861 gcagattggc gacgtgttac tgagcctttc gccatgcctc cgaagagaca ttaaacttgc
     1921 atgtctattg cgaatctgat agcacctagg tccggcatga tcaaccggct taattcgccc
     1981 tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     2041 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     2101 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     2161 acgcgctgga aggcagtaca ccttgatagg tgggctgccc ttcctggttg gcttggtttc
     2221 atcagccatc cgcttgccct catctgttac gccggcggta gccggccagc ctcgcagagc
     2281 aggattcccg ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg aacacccgct
     2341 cgcgggtggg cctacttcac ctatcctgcc cggctgacgc cgttggatac accaaggaaa
     2401 gtctacacga accctttggc aaaatcctgt atatcgtgcg aaaaaggatg gatataccga
     2461 aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg gaaaagcgct gcttccctgc
     2521 tgttttgtgg aatatctacc gactggaaac aggcaaatgc aggaaccctg tagcggcgca
     2581 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc tagcgcccta
     2641 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     2701 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     2761 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     2821 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     2881 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     2941 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaatt
     3001 cagggcgcaa gggctgctaa aggaagcgga acacgtagaa agccagtccg cagaaacggt
     3061 gctgaccccg gatgaatgtc agctactggg ctatctggac aagggaaaac gcaagcgcaa
     3121 agagaaagca ggtagcttgc agtgggctta catggcgata gctagactgg gcggttttat
     3181 ggacagcaag cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt gggaagccct
     3241 gcaaagtaaa ctggatggct ttcttgccgc caaggatctg atggcgcagg ggatcaagat
     3301 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     3361 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     3421 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     3481 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     3541 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     3601 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatcccac cttgctcctg
     3661 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     3721 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     3781 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     3841 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     3901 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     3961 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     4021 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     4081 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgaatt gaaaaaggaa
     4141 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     4201 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     4261 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     4321 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     4381 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     4441 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     4501 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     4561 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     4621 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     4681 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     4741 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     4801 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     4861 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     4921 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     4981 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     5041 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     5101 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     5161 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     5221 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     5281 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     5341 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     5401 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     5461 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     5521 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     5581 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     5641 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     5701 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     5761 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     5821 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     5881 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     5941 ggaag
//
