LOCUS       sequence-49119-e         961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PKMYT1
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgc accagttgca acctcgtcgt gtttcttttc gcggcgaggc ttcagaaacc
      121 ttgcagagcc caggttatga tccgtcccgc ccagaaagtt tctttcaaca gtcgttccaa
      181 cgcctcagcc gtctgggaca tggttcttac ggcgaagtat tcaaagtacg ctccaaagag
      241 gacggccgtt tgtatgcggt gaaacgctca atgagtccgt ttcgtggccc caaagatcgc
      301 gcccgtaagc tggcggaagt tggctctcac gagaaagtcg gccagcatcc gtgttgcgta
      361 cgcctggagc aagcctggga agaaggcggt attctgtacc tgcagacaga gctgtgcggc
      421 ccttcactgc aacagcactg tgaggcgtgg ggtgcaagtt tgccagaagc tcaagtgtgg
      481 ggttatctgc gtgacacctt gctggccttg gcacatctgc attcgcaggg tttggttcac
      541 ctggatgtca agcccgccaa catcttcctg ggccctcgtg gtcgttgtaa actgggcgat
      601 tttggcctgt tggtggagtt gggcactgcg ggtgctggtg aagtacaaga aggcgatccg
      661 cgttacatgg caccagaatt gctgcagggc tcttatggta cagccgcgga tgttttcagc
      721 ctgggcttga ctattctgga ggtagcttgc aatatggagc tgccccacgg cggcgaaggt
      781 tggcaacagt tgcgccaagg ttacctgccc cctgagttta ccgccggttt gtcatccgag
      841 ttgcgcagtg tactggtcat gatgctggag cccgatccga aactgcgcgc aacagctgaa
      901 gcattgctgg ctttgcccgt tttgcgccaa ccatgacagt aaaggtggat acggatccga
      961 a
//
