LOCUS       sequence-491198-       12758 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Evaluation of PtU6.2c3 promoter (Pol III promoter) sequence 
            variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana 
            benthamiana reporter line.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12758)
  AUTHORS   Deguchi M, Sinclair KM, Patel A, Coile M, Ortega MA, Bewg WP, Tsai 
            CJ
  TITLE     A compendium of nonredundant short polymerase III promoters for 
            CRISPR applications.
  JOURNAL   Plant Physiol. 2025 Jul 3;198(3):kiaf294. doi: 
            10.1093/plphys/kiaf294.
  PUBMED    40673482
REFERENCE   2  (bases 1 to 12758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2025-07-3"; volume: "198(3):kiaf294. doi"; pages: 
            " 10.1093/plphys/kiaf294"
COMMENT     Sequence Label: p305N-30
FEATURES             Location/Qualifiers
     source          1..12758
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(829..1977)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MAQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(2276..3070)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHLERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      3282..3913
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    3993..4017
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(4532..5326)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4643..4662)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5253..5272
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     misc_RNA        6352..6427
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             8111..12214
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8111..12214
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8111..12214
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     terminator      12254..12502
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    12521..12545
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag aggatctcag
      781 taaagcgctg gctgctgaac ccccagccgg aactgacccc acaaggccct agcgtttgca
      841 atgcaccagg tcatcattga cccaggcgtg ttccaccagg ccgctgcctc gcaactcttc
      901 gcaggcttcg ccgacctgct cgcgccactt cttcacgcgg gtggaatccg atccgcacat
      961 gaggcggaag gtttccagct tgagcgggta cggctcccgg tgcgagctga aatagtcgaa
     1021 catccgtcgg gccgtcggcg acagcttgcg gtacttctcc catatgaatt tcgtgtagtg
     1081 gtcgccagca aacagcacga cgatttcctc gtcgatcagg acctggcaac gggacgtttt
     1141 cttgccacgg tccaggacgc ggaagcggtg cagcagcgac accgattcca ggtgcccaac
     1201 gcggtcggac gtgaagccca tcgccgtcgc ctgtaggcgc gacaggcatt cctcggcctt
     1261 cgtgtaatac cggccattga tcgaccagcc caggtcctgg caaagctcgt agaacgtgaa
     1321 ggtgatcggc tcgccgatag gggtgcgctt cgcgtactcc aacacctgct gccacaccag
     1381 ttcgtcatcg tcggcccgca gctcgacgcc ggtgtaggtg atcttcacgt ccttgttgac
     1441 gtggaaaatg accttgtttt gcagcgcctc gcgcgggatt ttcttgttgc gcgtggtgaa
     1501 cagggcagag cgggccgtgt cgtttggcat cgctcgcatc gtgtccggcc acggcgcaat
     1561 atcgaacaag gaaagctgca tttccttgat ctgctgcttc gtgtgtttca gcaacgcggc
     1621 ttgcttggcc tcgctgacct gttttgccag gtcctcgccg gcggtttttc gcttcttggt
     1681 cgtcatagtt cctcgcgtgt cgatggtcat cgacttcgcc aaacctgccg cctcctgttc
     1741 gagacgacgc gaacgctcca cggcggccga tggcgcgggc agggcagggg gagccagttg
     1801 cacgctgtcg cgctcgatct tggccgtagc ttgctgggcc atcgagccga cggactggaa
     1861 ggtttcgcgg ggcgcacgca tgacggtgcg gcttgcgatg gtttcggcat cctcggcgga
     1921 aaaccccgcg tcgatcagtt cttgcctgta tgccttccgg tcaaacgtcc gattcattca
     1981 ccctccttgc gggattgccc cgactcacgc cggggcaatg tgcccttatt cctgatttga
     2041 tccgcctggt gccttggtgt ccagataatc caccttatcg gcaatgaagt cggtcccgta
     2101 gaccgtctgg ccgtccttct cgtacttggt attccgaatc ttgccctgca cgaataccag
     2161 cgaccccttg cccaaatact tgccgtgggc ctcggcctga gagccaaaac acttgatgcg
     2221 gaagaagtcg gtgcgctcct gcttgtcgcc agcatcgttg cgccacatct aggtactaaa
     2281 acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg atccccagta
     2341 agtcaaaaaa tagctcgaca tactgttctt ccccaatatc ctccctgatc gaccggacgc
     2401 agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca ataaagccac
     2461 ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa
     2521 gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg
     2581 gggtgtcttc ttcccagttt tcgcaatcca catcggccag atcgttattc agtaagtaat
     2641 ccaattcggc taagcggctg tctaagctat tcgtataggg acaatccgat atgtcgatgg
     2701 agtgaaagag cctgatgcac tccgcataca gctcgataat cttttcaggg ctttgttcat
     2761 cttcatactc ttccgagcaa aggacgccat cggcctcact catgagcaga ttgctccagc
     2821 catcatgccg ttcaaggtgc aggacctttg gaacaggcag ctttccttcc agccatagca
     2881 tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg tccgtcattt
     2941 ttaaatatag gttttcattt tctcccacca gcttatatac cttagcagga gacattcctt
     3001 ccgtatcttt tacgcagcgg tatttttcga tcagtttttt caattccggt gatattctca
     3061 ttttagccat ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc
     3121 taattataac aagacgaact ccaattcact gttccttgca ttctaaaact ttaaatacca
     3181 gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc
     3241 gattttgaaa ccacaattat gggtgatgct gccaacgcga gagcgggccg ggagggttcg
     3301 agaagggggg gcacccccct tcggcgtgcg cggtcacgcg cacagggcgc agccctggtt
     3361 aaaaacaagg tttataaata ttggtttaaa agcaggttaa aagacaggtt agcggtggcc
     3421 gaaaaacggg cggaaaccct tgcaaatgct ggattttctg cctgtggaca gcccctcaaa
     3481 tgtcaatagg tgcgcccctc atctgtcagc actctgcccc tcaagtgtca aggatcgcgc
     3541 ccctcatctg tcagtagtcg cgcccctcaa gtgtcaatac cgcagggcac ttatccccag
     3601 gcttgtccac atcatctgtg ggaaactcgc gtaaaatcag gcgttttcgc cgatttgcga
     3661 ggctggccag ctccacgtcg ccggccgaaa tcgagcctgc ccctcatctg tcaacgccgc
     3721 gccgggtgag tcggcccctc aagtgtcaac gtccgcccct catctgtcag tgagggccaa
     3781 gttttccgcg aggtatccac aacgccggcg gctgcggtgt ctcgcacacg gcttcgacgg
     3841 cgtttctggc gcgtttgcag ggccatagac ggccgccagc ccagcggcga gggcaaccag
     3901 cccggtgagc gtctctagtg gactgatggg ctgcctgtat cgagtggtga ttttgtgccg
     3961 agctgccggt cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacaaa
     4021 ttgacgattt aaatggtagg cgcggcttaa ttaagcgctt aattaagcgc ttaattaagc
     4081 gcttaataag ccgcgccgta ccccaatagt ctcgacagac acatagcacc taacattatt
     4141 gcttaaattt actatttttt tactatatta tactcaaccc aatgagcata aagactgtaa
     4201 aatctcaaat tcctgagaag catatttatc gatcccacag acttgatagt tccataatcc
     4261 atacgctgca gccaaattgc tagtgtgttg aacatttaac acgtagagaa ctagaaaaga
     4321 tataaaacta agattgatat ccaaaataga cgagaacaat aagcaaaaac tcttagtttt
     4381 gaaataaatc aacaatcccg agggttgtca catacatcaa aaacgaaaat ccatatagca
     4441 aaaaaaactc taaattaccg ttcgacaaaa agagaaaact gataggacat ttgctaaaca
     4501 ttaaaatcaa aatttggatc aagccgaatt ctcagaagaa ctcgtcaaga aggcgataga
     4561 aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc
     4621 attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt
     4681 ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga
     4741 tattcggcaa gcaggcatcg ccatgggtca cgacgagatc atcgccgtcg ggcatgcgcg
     4801 ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat
     4861 cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt
     4921 ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca
     4981 tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt
     5041 cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag
     5101 gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt tcattcaggg
     5161 caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca
     5221 cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat agcctctcca
     5281 cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcatctcg agacgtaccg
     5341 cgcgatgcat tcgaagatcg atccttcgcc tggaggagag aaatcagtgg cgctgcggct
     5401 tttagggttt ctttgttgat ggaatgagag tgtaagctct gccagtgcca ctttattagg
     5461 gttttacaag cccttttctt cgtaattggg cctgacattt tgtgccactt gggcctttag
     5521 agatgaaaat gtatattggg cttaagttga cttgaaggat aaattagttt aggatattac
     5581 gttttttatg agaattggtg tgtcggatac atgtatatga tgcattcaaa tatatgtatt
     5641 ctagatacat ttaagtttag atacaatcta aaatgtgtct ttaattacag gactgtaact
     5701 aaaatactta atgtaagaag aatattactc ctttaatagc ttttgagtat atctagtcta
     5761 acatctttta aaaaagtcta atttctttca tttatttttc gagcaatagc aaagtgcata
     5821 attatttttt tcttctagaa attcagattt gtttctctaa attttgagat tcttttctca
     5881 attttgtatg tctagagaac aatgtgtatt tttcactcta gttggttgtt actttgttga
     5941 atgttctgat aaaagtatat tgttatttct gaagtagata taaaccttca tttggaaatt
     6001 atacataaat caaaatcgtt aattatctag atcaagatat atgccctttt cctaatgtat
     6061 ttgatacatg cacctaattt cactagatgt atcttttcta ttttttaaat tatgaatagt
     6121 taattttttc catatgtgta tttgatacat acttcatgac tttaaaaaat taattatata
     6181 ccagatatat gtatttaaaa tttgttatgt atttaaagta tgtatatgat tattcgatat
     6241 taatctcttc gatgaaggcc tagatatccc acatcgtctg gttagagaaa acatgacgta
     6301 ttaagtaagc gaagcagaga cctgttagtt gcatgcccga aggctacgtc cgttttagag
     6361 ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag
     6421 tcggtgcttt ttttactagt ggagctcaac ttttgattcg ctatttgcag tgcacctgtg
     6481 gcgttcatca catcttttgt gacactgttt gcactggtca ttgctattac aaaggacctt
     6541 cctgatgttg aaggagatcg aaagtaagta actgcacgca taaccatttt ctttccgctc
     6601 tttggctcaa tccatttgac agtcaaagac aatgtttaac cagctccgtt tgatatattg
     6661 tctttatgtg tttgttcaag catgtttagt taatcatgcc tttgattgat cttgaatagg
     6721 ttccaaatat caaccctggc aacaaaactt ggagtgagaa acattgcatt cctcggttct
     6781 ggacttctgc tagtaaatta tgtttcagcc atatcactag ctttctacat gcctcaggtg
     6841 aattcatcta tttccgtctt aactatttcg gttaattaaa gcacgaacac cattactgca
     6901 tgtagaagct tgataaacta tcgccaccaa tttatttttg ttgcgatatt gttactttcc
     6961 tcagtatgca gctttgaaaa gaccaaccct cttatccttt aacaatgaac aggtttttag
     7021 aggtagcttg atgattcctg cacatgtgat cttggcttca ggcttaattt tccaggtaaa
     7081 gcattatgag atactcttat atctcttaca tacttttgag ataatgcaca agaacttcat
     7141 aactatatgc tttagtttct gcatttgaca ctgccaaatt cattaatctc taatatcttt
     7201 gttgttgatc tttggtagac atgggtacta gaaaaagcaa actacaccaa ggtaaaatac
     7261 ttttgtacaa acataaactc gttatcacgg aacatcaatg gagtgtatat ctaacggagt
     7321 gtagaaacat ttgattattg caggaagcta tctcaggata ttatcggttt atatggaatc
     7381 tcttctacgc agagtatctg ttattcccct tcctctagct ttcaatttca tggtgaggat
     7441 atgcagtttt ctttgtatat cattcttctt cttctttgta gcttggagtc aaaatcggtt
     7501 ccttcatgta catacatcaa ggatatgtcc ttctgaattt ttatatcttg caataaaaat
     7561 gcttgtacca attgaaacac cagctttttg agttctatga tcactgactt ggttctaacc
     7621 aaaaaaaaaa aaatgtttaa tttacatatc taaaagtagg tttagggaaa cctaaacagt
     7681 aaaatatttg tatattattc gaatttcact catcataaaa acttaaattg caccataaaa
     7741 ttttgtttta ctattaatga tgtaatttgt gtaacttaag ataaaaataa tattccgtaa
     7801 gttaaccggc taaaaccacg tataaaccag ggaacctgtt aaaccggttc tttactggat
     7861 aaagaaatga aagcccatgt agacagctcc attagagccc aaaccctaaa tttctcatct
     7921 atataaaagg agtgacatta gggtttttgt tcgtcctctt aaagcttctc gttttctctg
     7981 ccgtctctct cattcgcgcg acgcaaacga tcttcaggtg atcttctttc tccaaatcct
     8041 ctctcataac tctgatttcg tacttgtgta tttgagctca cgctctgttt ctctcaccac
     8101 agcagctagc atggacaaga agtactccat tgggctcgat atcggcacaa acagcgtcgg
     8161 ctgggccgtc attacggacg agtacaaggt gccgagcaaa aaattcaaag ttctgggcaa
     8221 taccgatcgc cacagcataa agaagaacct cattggcgcc ctcctgttcg actccgggga
     8281 gacggccgaa gccacgcggc tcaaaagaac agcacggcgc agatataccc gcagaaagaa
     8341 tcggatctgc tacctgcagg agatctttag taatgagatg gctaaggtgg atgactcttt
     8401 cttccatagg ctggaggagt cctttttggt ggaggaggat aaaaagcacg agcgccaccc
     8461 aatctttggc aatatcgtgg acgaggtggc gtaccatgaa aagtacccaa ccatatatca
     8521 tctgaggaag aagcttgtag acagtactga taaggctgac ttgcggttga tctatctcgc
     8581 gctggcgcat atgatcaaat ttcggggaca cttcctcatc gagggggacc tgaacccaga
     8641 caacagcgat gtcgacaaac tctttatcca actggttcag acttacaatc agcttttcga
     8701 agagaacccg atcaacgcat ccggagttga cgccaaagca atcctgagcg ctaggctgtc
     8761 caaatcccgg cggctcgaaa acctcatcgc acagctccct ggggagaaga agaacggcct
     8821 gtttggtaat cttatcgccc tgtcactcgg gctgaccccc aactttaaat ctaacttcga
     8881 cctggccgaa gatgccaagc ttcaactgag caaagacacc tacgatgatg atctcgacaa
     8941 tctgctggcc cagatcggcg accagtacgc agaccttttt ttggcggcaa agaacctgtc
     9001 agacgccatt ctgctgagtg atattctgcg agtgaacacg gagatcacca aagctccgct
     9061 gagcgctagt atgatcaagc gctatgatga gcaccaccaa gacttgactt tgctgaaggc
     9121 ccttgtcaga cagcaactgc ctgagaagta caaggaaatt ttcttcgatc agtctaaaaa
     9181 tggctacgcc ggatacattg acggcggagc aagccaggag gaattttaca aatttattaa
     9241 gcccatcttg gaaaaaatgg acggcaccga ggagctgctg gtaaagctta acagagaaga
     9301 tctgttgcgc aaacagcgca ctttcgacaa tggaagcatc ccccaccaga ttcacctggg
     9361 cgaactgcac gctatcctca ggcggcaaga ggatttctac ccctttttga aagataacag
     9421 ggaaaagatt gagaaaatcc tcacatttcg gataccctac tatgtaggcc ccctcgcccg
     9481 gggaaattcc agattcgcgt ggatgactcg caaatcagaa gagaccatca ctccctggaa
     9541 cttcgaggaa gtcgtggata agggggcctc tgcccagtcc ttcatcgaaa ggatgactaa
     9601 ctttgataaa aatctgccta acgaaaaggt gcttcctaaa cactctctgc tgtacgagta
     9661 cttcacagtt tataacgagc tgaccaaggt caaatacgtc acagaaggga tgagaaagcc
     9721 agcattcctg tctggagagc agaagaaagc tatcgtggac ctcctcttca agacgaaccg
     9781 gaaagttacc gtgaaacagc tcaaagaaga ctatttcaaa aagattgaat gtttcgactc
     9841 tgttgaaatc agcggagtgg aggatcgctt caacgcatcc ctgggaacgt atcacgatct
     9901 cctgaaaatc attaaagaca aggacttcct ggacaatgag gagaacgagg acattcttga
     9961 ggacattgtc ctcaccctta cgttgtttga agatagggag atgattgaag aacgcttgaa
    10021 aacttacgct catctcttcg acgacaaagt catgaaacag ctcaagaggc gccgatatac
    10081 aggatggggg cggctgtcaa gaaaactgat caatggcatc cgagacaagc agagtggaaa
    10141 gacaatcctg gattttctta agtccgatgg atttgccaac cggaacttca tgcagttgat
    10201 ccatgatgac tctctcacct ttaaggagga catccagaaa gcacaagttt ctggccaggg
    10261 ggacagtctt cacgagcaca tcgctaatct tgcaggtagc ccagctatca aaaagggaat
    10321 actgcagacc gttaaggtcg tggatgaact cgtcaaagta atgggaaggc ataagcccga
    10381 gaatatcgtt atcgagatgg cccgagagaa ccaaactacc cagaagggac agaagaacag
    10441 tagggaaagg atgaagagga ttgaagaggg tataaaagaa ctggggtccc aaatccttaa
    10501 ggaacaccca gttgaaaaca cccagcttca gaatgagaag ctctacctgt actacctgca
    10561 gaacggcagg gacatgtacg tggatcagga actggacatc aatcggctct ccgactacga
    10621 cgtggatcat atcgtgcccc agtcttttct caaagatgat tctattgata ataaagtgtt
    10681 gacaagatcc gataaaaata gagggaagag tgataacgtc ccctcagaag aagttgtcaa
    10741 gaaaatgaaa aattattggc ggcagctgct gaacgccaaa ctgatcacac aacggaagtt
    10801 cgataatctg actaaggctg aacgaggtgg cctgtctgag ttggataaag ccggcttcat
    10861 caaaaggcag cttgttgaga cacgccagat caccaagcac gtggcccaaa ttctcgattc
    10921 acgcatgaac accaagtacg atgaaaatga caaactgatt cgagaggtga aagttattac
    10981 tctgaagtct aagctggtct cagatttcag aaaggacttt cagttttata aggtgagaga
    11041 gatcaacaat taccaccatg cgcatgatgc ctacctgaat gcagtggtag gcactgcact
    11101 tatcaaaaaa tatcccaagc ttgaatctga atttgtttac ggagactata aagtgtacga
    11161 tgttaggaaa atgatcgcaa agtctgagca ggaaataggc aaggccaccg ctaagtactt
    11221 cttttacagc aatattatga attttttcaa gaccgagatt acactggcca atggagagat
    11281 tcggaagcga ccacttatcg aaacaaacgg agaaacagga gaaatcgtgt gggacaaggg
    11341 tagggatttc gcgacagtcc ggaaggtcct gtccatgccg caggtgaaca tcgttaaaaa
    11401 gaccgaagta cagaccggag gcttctccaa ggaaagtatc ctcccgaaaa ggaacagcga
    11461 caagctgatc gcacgcaaaa aagattggga ccccaagaaa tacggcggat tcgattctcc
    11521 tacagtcgct tacagtgtac tggttgtggc caaagtggag aaagggaagt ctaaaaaact
    11581 caaaagcgtc aaggaactgc tgggcatcac aatcatggag cgatcaagct tcgaaaaaaa
    11641 ccccatcgac tttctcgaag cgaaaggata taaagaggtc aaaaaagacc tcatcattaa
    11701 gcttcccaag tactctctct ttgagcttga aaacggccgg aaacgaatgc tcgctagtgc
    11761 gggcgagctg cagaaaggta acgagctggc actgccctct aaatacgtta atttcttgta
    11821 tctggccagc cactatgaaa agctcaaagg gtctcccgaa gataatgagc agaagcagct
    11881 gttcgtggaa caacacaaac actaccttga tgagatcatc gagcaaataa gcgagttctc
    11941 caaaagagtg atcctcgccg acgctaacct cgataaggtg ctttctgctt acaataagca
    12001 cagggataag cccatcaggg agcaggcaga aaacattatc cacttgttta ctctgaccaa
    12061 cttgggcgcg cctgcagcct tcaagtactt cgacaccacc atagacagaa agcggtacac
    12121 ctctacaaag gaggtcctgg acgccacact gattcatcag tcaattacgg ggctctatga
    12181 aacaagaatc gacctctctc agctcggtgg agacggaaca agaaagagaa gaaaggtgtg
    12241 acggatcctc gaatatgaag atgaagatga aatatttggt gtgtcaaata aaaagcttgt
    12301 gtgcttaagt ttgtgttttt ttcttggctt gttgtgttat gaatttgtgg ctttttctaa
    12361 tatcaaatga atgtaagatc tcattataat gaataaacaa atgtttctat aatccattgt
    12421 gaatgttttg ttggatctct tctgcagcat ataactactg tatgtgctat ggtatggact
    12481 atggaatatg attaaagata aggtttaaac tatcagtgtt tgacaggata tattggcggg
    12541 taaacctaag agaaaagagc gtttattaga ataatcggat atttaaaagg gcgtgaaaag
    12601 gtttatccgt tcgtccattt gtatgtgcat gccaaccaca ggagatccaa aaggatctag
    12661 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
    12721 tgagcgtcag accccgtaga aaagatcaaa ggatcttc
//
