LOCUS       sequence-491210-       11891 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of a bacterial bifunctional salicylate synthase 
            targeted to the cytosol.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11891)
  AUTHORS   Xue LJ, Guo W, Yuan Y, Anino EO, Nyamdari B, Wilson MC, Frost CJ, 
            Chen HY, Babst BA, Harding SA, Tsai CJ
  TITLE     Constitutively elevated salicylic acid levels alter photosynthesis 
            and oxidative state but not growth in transgenic populus.
  JOURNAL   Plant Cell. 2013 Jul;25(7):2714-30. doi: 10.1105/tpc.113.112839. 
            Epub 2013 Jul 31.
  PUBMED    23903318
REFERENCE   2  (bases 1 to 11891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.113.112839"; journalName: "Plant Cell"; date: 
            "2013-07"; volume: "25"; issue: "7"; pages: "2714-30"
COMMENT     Sequence Label: pCAMBIA1302-Pro35S:Irp9
FEATURES             Location/Qualifiers
     source          1..11891
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    775..915
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(841..860)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1001..1023
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      1259..1453
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(1519..2592)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3021..3650)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    4951..4975
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(4997..5249)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(5281..5298)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(5305..6015)
                     /codon_start=1
                     /gene="mgfp5"
                     /product="GFP with folding enhancement mutations"
                     /label=mgfp5
                     /note="suitable for expression in plants due to removal of 
                     a cryptic intron"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(5345..5365)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     5960..5988
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     promoter        complement(7389..7734)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             complement(7968..8195)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNRLA
                     AHPPFASWRNSEEARTDRPSQQLRSLNGEC"
     primer_bind     8096..8118
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8110..8127
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8111..8127
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    8131..8187
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(8196..8212)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8196..8212)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8209..8231)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8220..8236
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8244..8250,8251..8268,8269..8274))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8289..8310
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8501..9177
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             9244..10269
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    10310..10484
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    10562..10586
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             11011..11805
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
      841 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
      901 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
      961 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     1021 aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg
     1081 gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc
     1141 catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg
     1201 cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta
     1261 tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa
     1321 atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg
     1381 ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta
     1441 cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc
     1501 tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta
     1561 gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag
     1621 gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct
     1681 gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg
     1741 gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg
     1801 aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag
     1861 ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat
     1921 caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg
     1981 catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt
     2041 tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg
     2101 gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac
     2161 cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac
     2221 ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt
     2281 cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata
     2341 gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg
     2401 cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca
     2461 cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt
     2521 caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt
     2581 gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag
     2641 gcaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg
     2701 gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat
     2761 ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag
     2821 ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc
     2881 cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc
     2941 agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat
     3001 tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg
     3061 acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca
     3121 gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg
     3181 gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca
     3241 aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg
     3301 tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct
     3361 gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag
     3421 tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg
     3481 gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa
     3541 tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg
     3601 gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct
     3661 tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt
     3721 tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc
     3781 tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca
     3841 cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga
     3901 tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc
     3961 ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg
     4021 gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg
     4081 gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc
     4141 ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc
     4201 aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag
     4261 ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt
     4321 ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg
     4381 ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt
     4441 cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct
     4501 ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg
     4561 ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc
     4621 ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg
     4681 actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac
     4741 ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac
     4801 tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg
     4861 ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg
     4921 attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac
     4981 tgatagttta attcccgatc tagtaacata gatgacaccg cgcgcgataa tttatcctag
     5041 tttgcgcgct atattttgtt ttctatcgcg tattaaatgt ataattgcgg gactctaatc
     5101 ataaaaaccc atctcataaa taacgtcatg cattacatgt taattattac atgcttaacg
     5161 taattcaaca gaaattatat gataatcatc gcaagaccgg caacaggatt caatcttaag
     5221 aaactttatt gccaaatgtt tgaacgatcg gggaaattcg agctggtcac caattcacac
     5281 gtggtggtgg tggtggtggc tagctttgta tagttcatcc atgccatgtg taatcccagc
     5341 agctgttaca aactcaagaa ggaccatgtg gtctctcttt tcgttgggat ctttcgaaag
     5401 ggcagattgt gtggacaggt aatggttgtc tggtaaaagg acagggccat cgccaattgg
     5461 agtattttgt tgataatgat cagcgagttg cacgccgccg tcttcgatgt tgtggcgggt
     5521 cttgaagttg gctttgatgc cgttcttttg cttgtcggcc atgatgtata cgttgtggga
     5581 gttgtagttg tattccaact tgtggccgag gatgtttccg tcctccttga aatcgattcc
     5641 cttaagctcg atcctgttga cgagggtgtc tccctcaaac ttgacttcag cacgtgtctt
     5701 gtagttcccg tcgtccttga agaagatggt cctctcctgc acgtatccct caggcatggc
     5761 gctcttgaag aagtcgtgcc gcttcatatg atctgggtat cttgaaaagc attgaacacc
     5821 ataagagaaa gtagtgacaa gtgttggcca cggaacaggt agttttccag tagtgcaaat
     5881 aaatttaagg gtaagttttc cgtatgttgc atcaccttca ccctctccac tgacagaaaa
     5941 tttgtgccca ttaacatcac catctaattc aacaagaatt gggacaactc cagtgaaaag
     6001 ttcttctcct ttactagtaa cggccgccag tgtgctggaa ttcgcccttg gctagcctac
     6061 accattaaat agggcgcaat gctcgctaat ttctcccggg tttccgtcag ttcgcgttcc
     6121 ggtgtagatt gcgcgatgat tcccgccccc gcctgtatcc agcagcgctg gctgtcctga
     6181 aatacggaac gcagaactag cgccgcatcg aagcgcgtat cgtccagcag cagaattgcg
     6241 ccggaataaa gctctcgcgg cgtcttctca atttgcataa tcgcgttcag ggcagcgttt
     6301 ttagggatac ctgaggcggt aatcgacgga aacagcacgg taaacgcatc ccaggcatcc
     6361 ttgttttccg caagctgacc gctcacgcca gaccccagat gctgaacgct gccgcgctgg
     6421 cgaaccgaca ttaaatcttc aaccaccacg ctgcccggca ggcaaacggc ctccagttca
     6481 gcaatagctt ctttgacaga aaggatatgc tcaagcacct ctttactatc gtgcagcagt
     6541 tctgcctctt tcgccttatt atgctccggg tttcccatgc gatcgcgggt gcccgcaagc
     6601 ggttcagtaa ccactttatt gcccgtcact gacatcacca gttccgggct aaagcccagc
     6661 gcttcgcgtc cttcctgacg gaacataaac gagcgcactg gcgtgtttgc ctgtcgcccg
     6721 tataacagcg tggcgggcat atcaatccgc gatggcaggg gaatggcgcg cgagacaatc
     6781 actttgacat actcgccacg gcggatctcc gcaacggcgc gcgcaacctg ttgtttatac
     6841 gcctcaccat tgagggcggt atccaccgcc agtggtgcgt tctgcgtcgt tgtactggcc
     6901 tcttttaccc actcgcacaa acgtcggcac ccgtcggcgg agtccgcata aacggtgaca
     6961 tttccctttt caaaaataag ttcttcacgg ggaacggtta acgtcagcag cggccactcc
     7021 ccggcgttaa aggcaatccc ccgcgcatgc gccgcaaagt tgaacccaac ctgtccatat
     7081 acccgacgtc ctttcacctg aggatgtgcc ataaaacgac gcgcgcagtc ggcaatggaa
     7141 tccaccgccc atttttgctc cccggcatca tcaataaatg tcgcctgcgt tccgtcggca
     7201 ttaatgtgca gccgtgcctg gcaccctttg cctaaatacc aacagggttg gcgctcatag
     7261 acataacact cttcttctgc gaactggcgt aaaacgccag aaatcgtcgg cagccattgt
     7321 tcctctggta acgccaggtg tagaaattca ctgattttca tagatctacc atggtcaaga
     7381 gtcccccgtg ttctctccaa atgaaatgaa cttccttata tagaggaagg gtcttgcgaa
     7441 ggatagtggg attgtgcgtc atcccttacg tcagtggaga tatcacatca atccacttgc
     7501 tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca
     7561 tctttgggac cactgtcggc agaggcatct tcaacgatgg cctttccttt atcgcaatga
     7621 tggcatttgt aggagccacc ttccttttcc actatcttca caataaagtg acagatagct
     7681 gggcaatgga atccgaggag gtttccggat attacccttt gttgaaaagt ctcaattgcc
     7741 ctttggtctt ctgagactgt atctttgata tttttggagt agacaagtgt gtcgtgctcc
     7801 accatgttga cgaagatttt cttcttgtca ttgagtcgta agagactctg tatgaactgt
     7861 tcgccagtct ttacggcgag ttctgttagg tcctctattt gaatctttga ctccatgaag
     7921 ctaaactgaa ggcgggaaac gacaatctga tccaagctca agctgctcta gcattcgcca
     7981 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     8041 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     8101 tcacgacgtt gtaaaacgac ggccagtgcc aagcttgcat gcctgcaggt cgactctaga
     8161 ggatccccgg gtaccgagct cgaattcgta atcatgtcat agctgtttcc tgtgtgaaat
     8221 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     8281 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     8341 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     8401 ttgcgtattg gctagagcag cttgccaaca tggtggagca cgacactctc gtctactcca
     8461 agaatatcaa agatacagtc tcagaagacc aaagggctat tgagactttt caacaaaggg
     8521 taatatcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc atcaaaagga
     8581 cagtagaaaa ggaaggtggc acctacaaat gccatcattg cgataaagga aaggctatcg
     8641 ttcaagatgc ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg
     8701 tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt gaacatggtg
     8761 gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga agaccaaagg
     8821 gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt ccattgccca
     8881 gctatctgtc acttcatcaa aaggacagta gaaaaggaag gtggcaccta caaatgccat
     8941 cattgcgata aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg tcccaaagat
     9001 ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag
     9061 caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct
     9121 tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac acgctgaaat
     9181 caccagtctc tctctacaaa tctatctctc tcgagctttc gcagatccgg ggggcaatga
     9241 gatatgaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc
     9301 gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc
     9361 gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa
     9421 gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac
     9481 attggggagt ttagcgagag cctgacctat tgcatctccc gccgttcaca gggtgtcacg
     9541 ttgcaagacc tgcctgaaac cgaactgccc gctgttctac aaccggtcgc ggaggctatg
     9601 gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa
     9661 ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg
     9721 tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat
     9781 gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc
     9841 ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag
     9901 gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg
     9961 gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg
    10021 ccacgactcc gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt
    10081 gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc
    10141 ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat
    10201 ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca
    10261 aagaaataga gtagatgccg accgggatct gtcgatcgac aagctcgagt ttctccataa
    10321 taatgtgtga gtagttccca gataagggaa ttagggttcc tatagggttt cgctcatgtg
    10381 ttgagcatat aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa
    10441 atttctaatt cctaaaacca aaatccagta ctaaaatcca gatcccccga attaattcgg
    10501 cgttaattca gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt
    10561 tgtttacacc acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg
    10621 gcacaaaatc accactcgat acaggcagcc catcagtccg ggacggcgtc agcgggagag
    10681 ccgttgtaag gcggcagact ttgctcatgt taccgatgct attcggaaga acggcaacta
    10741 agctgccggg tttgaaacac ggatgatctc gcggagggta gcatgttgat tgtaacgatg
    10801 acagagcgtt gctgcctgtg atcaccgcgg tttcaaaatc ggctccgtcg atactatgtt
    10861 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
    10921 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
    10981 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
    11041 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
    11101 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
    11161 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
    11221 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
    11281 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
    11341 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
    11401 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
    11461 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
    11521 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
    11581 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
    11641 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
    11701 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
    11761 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agaatgcatg
    11821 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
    11881 aaaggatctt c
//
