LOCUS       sequence-49125-e        1183 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1183)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: VRK1
FEATURES             Location/Qualifiers
     source          1..1183
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgc gtgtaaaagc agctcaagct ggaagacaga gctctgcaaa gagacatctt
      121 gcagaacaat ttgcagttgg agagataata actgacatgg cagcagcggc atggaaagta
      181 ggattaccca ttggccaagg aggctttggc tgtatatatc ttgctgatat gaattcttca
      241 gagtcagttg gcagtgatgc accttgtgtt gtaaaagtgg aacccagtga caatggacct
      301 ctttttactg aattaaagtt ctaccaacga gctgcaaaac cagagcaaat tcagaaatgg
      361 attcgtaccc gtaagctgaa gtacctgggt gttcctaagt attgggggtc tggtctacat
      421 gacaaaaatg gaaaaagtta caggtttatg ataatggatc gctttgggag tgaccttcag
      481 aaaatatatg aagcaaatgc caaaaggttt tctcggaaaa ctgtcttgca gctaagctta
      541 agaattctgg atattctgga atatattcac gagcatgagt atgtgcatgg agatatcaag
      601 gcctcaaatc ttcttctgaa ctacaagaat cctgaccagg tgtacttggt agattatggc
      661 cttgcttatc ggtactgccc agaaggagtt cataaagcat acgcagcaga ccccaaaaga
      721 tgtcacgatg gcactattga attcacgagc atcgatgcac acaatggcgt ggccccatca
      781 agacgtggtg atttggaaat acttggttat tgcatgatcc aatggcttac tggccatctt
      841 ccttgggagg ataatttgaa agatcctaaa tatgttagag attccaaaat tagatacaga
      901 gaaaatattg caagtttgat ggacaaatgt tttcctgcgg caaacgcacc aggtgaaatt
      961 gccaaataca tggaaacagt gaaattacta gactacactg aaaaacctct ttatgaaaat
     1021 ttacgtgaca ttcttttgca aggactaaaa gctataggaa gtaaggatga tggcaaattg
     1081 gacctcagtg ttgtggagaa tggaggtttg aaagcaaaaa caataacaaa gaagcgagcg
     1141 gcagaaattg aagaatgaca gtaaaggtgg atacggatcc gaa
//
