LOCUS       sequence-491297-        6501 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of HseIF4AI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6501)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 6501)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-HseIF4AI_T
FEATURES             Location/Qualifiers
     source          1..6501
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    127..261
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(164..183)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     218..237
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(1508..1534)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(1559..1624)
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     promoter        complement(1644..4153)
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     primer_bind     complement(4217..4235)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4303..4407
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4408..4476,4477..5268)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4626..4645)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5439..6027
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5928..5947
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctcaagggca tcggtcgact gatcataatc agccatacca catttgtaga ggttttactt
       61 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
      121 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
      181 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
      241 gtatcttatc atgtctggat cccggtcaga tgaggtcagc aacattgagg ggcatttcct
      301 caatggaggt gttgtagaag gtctcaatgt ctcgaagagt cctcttgtct tcttctgtca
      361 ccatgttaat agccacacct ttacggccaa accgtccacc tcgaccgatt ctgtggatat
      421 agttttccct gttggtggga aggtcatagt tgatgactaa agaaacctgc tgcacatcaa
      481 tgcctctggc cagcaggtca gtggtaatca aaactctgct agagccagaa cgaaactccc
      541 tcataatcac gtctcgttcc ttttggtcca tatctccatg catggcggat acagtgaaat
      601 ctcgagcatg catcttctcg gtgagccagt ccaccttcct ccgggtgttg atgaagatga
      661 ctgcctgggt gatggtcagg gtttcataca agtcacatag tgtgtccagc ttccactcct
      721 ctcgttccac gttgatgtag aactggcgga taccctccag ggtcaactct tccttcttga
      781 caagaatccg aatggggtcc ctcatgaact tcttggtcac ctcaagcaca tcagaaggca
      841 ttgtggctga cagcaaaact acctgggtgt tgctgttgag cttttggaat atgtcataga
      901 tctggtcctt gaatccacgg cttaacattt cgtcagcttc atccagtaca aacatcttga
      961 tgtatttggg ggacaggtat ctccggttaa gcatatcaaa tacacggcca ggggtaccca
     1021 cgatgatgtg gggagcttcc atctgcagtt tctgcacctc agcacgcacg ttggtgcccc
     1081 cgatacaggc gtgacaggag gcgcccatgt agtctcctag tgccatgacc accttctgta
     1141 tctgctgagc caattctcga gtgggtgcta ggaccaaggc ctgggtggct tttagatcta
     1201 attcaatctg ctgcagaatt gatatggcaa atgtggccgt tttcccagtc ccagattggg
     1261 cttgagcaat cacatcataa cccttgatac aaggtagaat ggctcgctgc tggatggcag
     1321 agggcttctc aaaaccatag gcgtagatgc cacggagaag ggactccgag aggttcatgt
     1381 catcaaagct gtcaacaatc tcattccagt tactctcgat gacgccttcg ggctccatcc
     1441 catcggggcc attgtctctg gatcgggaat cctggctcgc agacattgca gaattcaagc
     1501 ttggaggagc gtaatctgga acatcgtatg ggtaagccat atgtggagga gatccaccgt
     1561 ttgcagcttt ccattgagct tgtttctcag cgcgacgctc acgtcgtcgt gtttgtgcgt
     1621 ccatgttggt accccgatcc ggggtctctg gattagacga ctgctggctg atggagcggc
     1681 tttgtgtcgg gaggagtatc cacacagcac aagaactcaa acggtagtga tatgaaaact
     1741 ggtcccgcag cccgttttat aaagcccgct tagcgctaaa accttatcag ccgcaatgct
     1801 ctccgctctc aagtcgcgtt caaaactttt accatatatg ggtatgaatt gttcgccgat
     1861 tgggttttca tttacggaga atttcctccg caactgggtg ttcgtgcttg tgtgtgtctt
     1921 gtatatcgcc atgaagagtg tacttccatg ggtcctatca gttgcggcat gtgattcaat
     1981 ttgggggtta ctcatattcg atacttcagc tggttatctt tatgctgtga tggtcacggt
     2041 gcttttacac attttttccc caactactca ttgtatgcca aaacatacat atgtacatgt
     2101 gtttatagtg ggtttattag taatgtccag cgatattaaa acattacagt taaataatat
     2161 tcatattagc ttacgtttac tgctatctgc acttcaaatt ggttattcca gggacatttt
     2221 gtgcaacaca catcttctag tagggcattc actagtcgta gaaaacacac ctaacttaag
     2281 ccggttttaa caataacggc gaaacagctg ccagacgaga gaaaagtaca cagcaagaag
     2341 catttctata atgatgccgt cctaaatatg cagcattttt gctcaaattg atcatttcgt
     2401 tgcatcgctc aggtggtata gtatttggct attgaatgtg attgctatct taatagtttt
     2461 gaatagtaat agtgcaaata ttcattcata aaaaataggt aatcgatttt ttcagtgcag
     2521 tctatgcgtt tgcgcaacag agacgaagag agagacgcct cacacacaag cacatgcggt
     2581 ctaagctgct ctccctcttt ttctctctct ctctggcttg cggcagtttt ccagaggctg
     2641 tgacttagta agcatttctt gttgttaccc taacgataag cgtccgattc ctaattctct
     2701 ttcaaataat ttgttttatt tttttttttg tgtgttttga agtcaaacag cttcataaac
     2761 aatattaaat atcgcaattg ttattgcaaa tgcattcgta tcaggcgcct tgatgttttt
     2821 cgccaaaact tcgtctgctc tttgtcagcg taattgcgtt tgccggtcgt gccgtcttgc
     2881 tcgcacgcgg cttgcaaaca aaaagtgaaa gagggaaagc cacctgtgtt gttgcttttg
     2941 ttgctatcgt tggctgcaca cacatggcaa taagtaacca tattgtagac ttatttattt
     3001 tagcgttttc gttgactaat ctttgtttaa ataattgcct atttcgagcc aaaatgacgc
     3061 ataaaaggaa acggcaacat gcggtgcgtt gagcatgcgt aagcgagatg gcgctagaga
     3121 gagcgcagca taaaaagaaa aaaacaaccg gcgagtggcg agacacgaat aatacacaca
     3181 cgcacacatg gtcaaaggag tggctccaac gtcggtgacg tcgcgcccag ccacagaccc
     3241 cgccacgccc cgccccaccc ttccttccgc tgccccgcac ccctttcctt ccactaccgt
     3301 tttgccgcat ttggtattgc catctcgctt ttttggcttt actttgcttt ttgaataagc
     3361 caagaaactg aaatcaaagc ggacagtccg aaaattgaat attccaaaaa acaacaattg
     3421 cacatgcact gcgttcggca aaagacgcag aaattcaagc cttttggtct ggcatcatct
     3481 taaaatatca tcagtatcat cattgccatt ataaatacat aagaaaatga gaatacatta
     3541 aaggtatata catacaatct ttgtcatagt caacatattt tgcaacaaaa ttatctatag
     3601 ataacatgtt tttgtagaaa attccactaa ctgattttcg tataaataca aattattctc
     3661 attcttcaaa atactcgccg acgtcatcgc gaaattcctc agcattcttc gacaaatgaa
     3721 gagagacagc gagtgacaca atcttatctt gccgacgagt ctttgaaaat ggccccgggt
     3781 aacggtgaaa aacatatagc caagaatgga gcgatcgcca ggcgatggaa attggcttca
     3841 aattgctccg aatatggcca gttggtcagt tggcattgga ttgtggagag atactagatc
     3901 agctcgttct ccttctcctc cgcattctcc tactccgccc gaagacgctt ctttgatcgc
     3961 ttctttctcg aatgtgtaca tattcattat gtatttggtg tttggcgacg atcagccagc
     4021 tttaatggcc ataaaacgtg tcttggtttg tgggccacat aacaggcagc gagatctgga
     4081 aaaatgatgt gacagtggaa atgagaagta ggtgcatctg caaagatttg attcagagtt
     4141 gatgccattc atgatttttt tttagaagta tcatttgtgt ttttagaata tagaattaat
     4201 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     4261 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     4321 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     4381 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     4441 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     4501 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     4561 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     4621 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     4681 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     4741 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     4801 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     4861 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     4921 ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta
     4981 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     5041 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     5101 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     5161 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     5221 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     5281 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     5341 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     5401 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     5461 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     5521 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     5581 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     5641 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     5701 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     5761 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     5821 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     5881 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     5941 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     6001 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     6061 gccttttgct ggccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg
     6121 gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc
     6181 atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg
     6241 gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga
     6301 gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag
     6361 cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc
     6421 atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag
     6481 gagctgactg ggttgaaggc t
//
