LOCUS       sequence-49130-e        1396 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1396)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1396)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ENO3
FEATURES             Location/Qualifiers
     source          1..1396
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg ccatgcagaa aatctttgcc cgggaaatct tggactccag gggcaacccc
      121 acggtggagg tggacctgca cacggccaag ggccgattcc gagcagctgt gcccagtggg
      181 gcttccacgg gtatctatga ggctctggaa ctaagagacg gagacaaagg ccgctacctg
      241 gggaaaggag tcctgaaggc tgtggagaac atcaacagta ctctgggccc tgctctgctg
      301 caaaagaaac taagcgttgc ggatcaagaa aaagttgaca aatttatgat tgagctagat
      361 gggaccgaga ataagtccaa gtttggggcc aatgccatcc tgggcgtgtc cttggccgtg
      421 tgtaaggcgg gagcagctga gaagggggtc cccctgtacc gccacatcgc agatctcgct
      481 gggaaccctg acctcatact cccagtgcca gccttcaatg tgatcaacgg gggctcccat
      541 gctggaaaca agctggccat gcaggagttc atgattctgc ctgtgggagc cagctccttc
      601 aaggaagcca tgcgcattgg cgccgaggtc taccaccacc tcaagggggt catcaaggcc
      661 aagtatggga aggatgccac caatgtgggt gatgaaggtg gcttcgcacc caacatcctg
      721 gagaacaatg aggccctgga gctgctgaag acggccatcc aggcggctgg ttacccagac
      781 aaggtggtga tcggcatgga tgtggcagca tctgagttct atcgcaatgg gaagtacgat
      841 cttgacttca agtcgcctga tgatcccgca cggcacatca ctggggagaa gctcggagag
      901 ctgtataaga gctttatcaa gaactatcct gtggtctcca tcgaagaccc ctttgaccag
      961 gatgactggg ccacttggac ctccttcctc tcgggggtga acatccagat tgtgggggat
     1021 gacttgacag tcaccaaccc caagaggatt gcccaggccg ttgagaagaa ggcctgcaac
     1081 tgtctgctgc tgaaggtcaa ccagatcggc tcggtgaccg aatcgatcca ggcgtgcaaa
     1141 ctggctcagt ctaatggctg gggggtgatg gtgagccacc gctctgggga gactgaggac
     1201 acattcattg ctgaccttgt ggtggggctc tgcacaggac agatcaagac tggcgccccc
     1261 tgccgctcgg agcgtctggc caaatacaac caactcatga ggatcgagga ggctcttggg
     1321 gacaaggcaa tctttgctgg acgcaagttc cgtaacccga aggccaagtg acagtaaagg
     1381 tggatacgga tccgaa
//
