LOCUS       sequence-49133-e        1193 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1193)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: STK25
FEATURES             Location/Qualifiers
     source          1..1193
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             31..201
                     /codon_start=1
                     /product="Z domain of staphylococcal protein A, engineered 
                     to contain multiple positive charges (Hedhammar and Hober, 
                     2007)"
                     /label=Z-basic tag
                     /note="useful for purifying tagged proteins by 
                     cation-exchange chromatography"
                     /translation="VDNKFNKERRRARREIRHLPNLNREQRRAFIRSLRDDPSQSANLL
                     AEAKKLNDAQPK"
     CDS             208..228
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1173..1190
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtggataaca agttcaacaa ggagcgtcga
       61 agagctcgcc gtgaaattcg ccatctgccg aacctgaacc gcgaacagcg tcgcgcattt
      121 attcgcagcc tgcgcgatga tccgagccag agcgcgaacc tgctggcgga agcgaagaag
      181 ctgaacgatg cgcagccgaa gggtaccgag aacctgtact tccaatccat ggctcacctc
      241 cggggatttg ccaaccagca ctctcgagtg gaccctgagg agctcttcac caagctcgac
      301 cgcattggca agggctcgtt tggggaggtc tacaagggca tcgataacca cacaaaggag
      361 gtggtggcca tcaagatcat cgacctggag gaggccgagg atgagatcga ggacatccag
      421 caggagatca ctgtcctcag tcagtgcgac agcccctaca tcacccgcta ctttggctcc
      481 tacctaaaga gcaccaagct atggatcatc atggagtacc tgggcggcgg ctcagcactg
      541 gacttgctta aaccaggtcc cctggaggag acatacattg ccacgatcct gcgggagatt
      601 ctgaagggcc tggattatct gcactccgaa cgcaagatcc accgagacat caaagctgcc
      661 aacgtgctac tctcggagca gggtgacgtg aagctggcgg actttggggt agcagggcag
      721 ctcacagaca cgcagattaa gaggaacaca ttcgtgggca cccccttctg gatggcacct
      781 gaggtcatca agcagtcggc ctacgacttc aaggctgaca tctggtccct ggggatcaca
      841 gccatcgagc tggccaaggg ggagcctcca aactctgacc tccaccccat gcgcgtcctg
      901 ttcctgattc ccaagaacag cccacccaca ctggagggcc agcacagcaa gcccttcaag
      961 gagttcgtgg aggcctgcct caacaaagac ccccgattcc ggcccacggc caaggagctc
     1021 ctgaagcaca agttcatcac acgctacacc aagaagacct ccttcctcac ggagctcatc
     1081 gaccgctata agcgctggaa gtcagagtga cagtaaaggt ggatacggat ccgaattcga
     1141 gctccgtcga caagcttgcg gccgcactcg agcaccacca ccaccaccac tga
//
