LOCUS       sequence-49135-e         697 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 697)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: TP73
FEATURES             Location/Qualifiers
     source          1..697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             626..646
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             647..664
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             665..688
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cttaagaagg agatatacta tggcgcctgt catcccctcc aacaccgact accccggacc
       61 ccaccacttt gaggtcactt tccagcagtc cagcacggcc aagtcagcca cctggacgta
      121 ctccccgctc ttgaagaaac tctactgcca gatcgccaag acatgcccca tccagatcaa
      181 ggtgtccacc ccgccacccc caggcactgc catccgggcc atgcctgttt acaagaaagc
      241 ggagcacgtg accgacgtcg tgaaacgctg ccccaaccac gagctcggga gggacttcaa
      301 cgaaggacag tctgctccag ccagccacct catccgcgtg gaaggcaata atctctcgca
      361 gtatgtggat gaccctgtca ccggcaggca gagcgtcgtg gtgccctatg agccaccaca
      421 ggtggggacg gaattcacca ccatcctgta caacttcatg tgtaacagca gctgtgtagg
      481 gggcatgaac cggcggccca tcctcatcat catcaccctg gagatgcggg atgggcaggt
      541 gctgggccgc cggtcctttg agggccgcat ctgcgcctgt cctggccgcg accgaaaagc
      601 tgatgaggac cactaccggg aggcagagaa cctctacttc caatcgcacc atcatcacca
      661 ccatgattac aaggatgacg acgataagtg aggatcc
//
