LOCUS       sequence-49136-e        1042 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1042)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: CBLC
FEATURES             Location/Qualifiers
     source          1..1042
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             971..991
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             992..1009
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1010..1033
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cttaagaagg agatatacta tggggcgaca gtgggaagag gcccgcgccc tgggccgggc
       61 agtcaggatg ctgcagcgcc tagaagagca atgcgtcgac ccccggctgt ccgtgagtcc
      121 cccttcgctg cgggacctgc tgccccgcac agcgcagctg cttcgagagg tggcccattc
      181 tcggcgggag gccggcggag gcggccccgg gggtcccggc ggctctgggg actttctact
      241 catctacctg gccaatctgg aggccaagag caggcaggtg gccgcgctgc tgcctccccg
      301 gggccgaagg agtgccaacg acgagctctt ccgggcgggc tccagactca ggcgacagct
      361 ggccaagctg gccatcatct tcagccacat gcacgcagag ctgcacgcac tcttccccgg
      421 gggaaagtac tgtggacaca tgtaccagct caccaaggcc cccgcccaca ccttctggag
      481 ggaaagttgc ggagcccggt gtgtgctgcc ctgggctgag tttgagtccc tcctgggcac
      541 ctgccaccct gtggaaccag gctgcacagc cctggccttg cgcaccacca ttgacctcac
      601 ctgcagcggg cacgtgtcca tcttcgagtt cgacgtcttc accaggctct ttcagccatg
      661 gccaacactc ctcaagaact ggcagctcct ggcagtcaac cacccaggct acatggcctt
      721 cctcacctat gatgaggtcc aagagcgtct gcaggcctgc agggacaagc caggcagtta
      781 catcttccgg cccagctgta ctcgcctggg gcagtgggcc atcggctatg tgagctcaga
      841 tggcagcatc ctgcagacca tccctgccaa caaacccctg tcccaggtgc tcctggaggg
      901 acagaaggac ggcttctacc tctacccaga tggaaagacc cacaacccag acctgactga
      961 gctcggcgca gagaacctct acttccaatc gcaccatcat caccaccatg attacaagga
     1021 tgacgacgat aagtgaggat cc
//
