LOCUS       sequence-49137-e        1102 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1102)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: TH
FEATURES             Location/Qualifiers
     source          1..1102
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1031..1051
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1052..1069
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1070..1093
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cttaagaagg agatatacta tggtcccctg gttcccaaga aaagtgtcag agctggacaa
       61 gtgtcatcac ctggtcacca agttcgaccc tgacctggac ttggaccacc cgggcttctc
      121 ggaccaggtg taccgccagc gcaggaagct gattgctgag atcgccttcc agtacaggca
      181 cggcgacccg attccccgtg tggagtacac cgccgaggag attgccacct ggaaggaggt
      241 ctacaccacg ctgaagggcc tctacgccac gcacgcctgc ggggagcacc tggaggcctt
      301 tgctttgctg gagcgcttca gcggctaccg ggaagacaat atcccccagc tggaggacgt
      361 ctcccgcttc ctgaaggagc gcacgggctt ccagctgcgg cctgtggccg gcctgctgtc
      421 cgcccgggac ttcctggcca gcctggcctt ccgcgtgttc cagtgcaccc agtatatccg
      481 ccacgcgtcc tcgcccatgc actcccctga gccggactgc tgccacgagc tgctggggca
      541 cgtgcccatg ctggccgacc gcaccttcgc gcagttctcg caggacattg gcctggcgtc
      601 cctgggggcc tcggatgagg aaattgagaa gctgtccacg ctgtactggt tcacggtgga
      661 gttcgggctg tgtaagcaga acggggaggt gaaggcctat ggtgccgggc tgctgtcctc
      721 ctacggggag ctcctgcact gcctgtctga ggagcctgag attcgggcct tcgaccctga
      781 ggctgcggcc gtgcagccct accaagacca gacgtaccag tcagtctact tcgtgtctga
      841 gagcttcagt gacgccaagg acaagctcag gagctatgcc tcacgcatcc agcgcccctt
      901 ctccgtgaag ttcgacccgt acacgctggc catcgacgtg ctggacagcc cccaggccgt
      961 gcggcgctcc ctggagggtg tccaggatga gctggacacc cttgcccatg cgctgagtgc
     1021 cattggcgca gagaacctct acttccaatc gcaccatcat caccaccatg attacaagga
     1081 tgacgacgat aagtgaggat cc
//
