LOCUS       sequence-49138-e         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MAT2B
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             928..948
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             949..966
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             967..990
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgaaccgtc gcgttctggt taccggtgct actggtctgc tgggtcgtgc agtacacaag
       61 gagtttcagc agaataactg gcacgctgta ggttgcggtt ttcgtcgtgc gcgtccaaaa
      121 ttcgagcaag taaacctgct ggactctaac gctgttcacc acatcatcca cgatttccag
      181 ccgcacgtta ttgtgcattg cgctgctgaa cgtcgtcctg acgttgtaga aaaccagcca
      241 gatgctgcct cccaactgaa cgttgatgcg tctggtaacc tggccaagga agctgctgca
      301 gtaggcgcat ttctgatcta tatcagctcc gattacgttt tcgacggtac caacccgcct
      361 tatcgtgagg aagacatccc ggctccactg aacctgtacg gtaagacgaa gctggatggc
      421 gaaaaagcgg tgctggaaaa taacctgggt gctgcagttc tgcgtatccc gatcctgtat
      481 ggtgaagtcg aaaaactgga agaatccgcg gttactgtta tgttcgacaa ggtccagttc
      541 tccaataagt ctgcaaacat ggaccattgg caacagcgct tcccgactca cgttaaagat
      601 gtggctacgg tttgccgcca actggcagag aagcgtatgc tggacccgag catcaagggt
      661 actttccatt ggagcggcaa cgaacagatg acgaaatacg aaatggcatg tgcgattgcc
      721 gacgcattca acctgccatc ttctcacctg cgtccgatca ccgattcccc agtgctgggt
      781 gcacaacgtc cacgtaatgc gcaactggac tgttctaaac tggaaactct gggtatcggt
      841 cagcgcactc catttcgtat cggtattaag gagtctctgt ggccgttcct gatcgataaa
      901 cgttggcgtc agactgtgtt ccatgcagag aacctctact tccaatcgca ccatcatcac
      961 caccatgatt acaaggatga cgacgataag tgaggatcc
//
