LOCUS       sequence-49139-e        1447 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1447)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MLYCD
FEATURES             Location/Qualifiers
     source          1..1447
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg acgagctgct gcgccgcgcg gtgccgccga cgccggccta cgagctgcgc
      121 gcagcaacac cggcgcccgc cgagggtcag tgcgcggact tcgtgagctt ctacggtggg
      181 ctggccgaga cggcccagcg ggccgaactg ctgggccgcc tggcgcgggg cttcggcgtg
      241 gaccacggcc aggtggcgga gcagagcgcc ggcgtgctcc atctgcgcca gcagcagcgg
      301 gaggcggcgg tgctgctgca ggccgaggac cggctgcgct acgcgctggt gccgcgctat
      361 cgcggcctct tccaccacat cagcaagctg gacggcggcg tgcgcttcct ggtgcagctg
      421 cgggccgacc tgctggaggc gcaggccctc aagctggtgg aggggccgga cgtccgggaa
      481 atgaatgggg tgctgaaagg aatgctctca gaatggtttt cccccgggtt cctgaaccta
      541 gaacgggtta cctggcattc accgtgtgaa gtgcttcaga aaatcagtga ggctgaggct
      601 gtgcatcctg taaaaaactg gatggacatg aagcgccgcg ttgggcccta cagaaggtgt
      661 tacttctttt ctcactgttc gacccctggg gagcccctgg tcgttttgca cgtggcactg
      721 actggtgaca tctccagcaa catccaggca atcgtgaagg aacatcctcc atcagaaaca
      781 gcagctgcta acaaaatcac tgctgcgatc ttttattcca tcagcttgac ccagcaggga
      841 ctccaagggg tggagctggg aacattcctc ataaagcgag tcgtcaagga gttgcagaga
      901 gagtttcctc accttggggt gttttcaagt ctgtcaccta tacctggttt caccaaatgg
      961 cttctggggc ttctgaactc gcaaacgaag gagcatggga ggaatgaact ctttacagat
     1021 tcggaatgta aggaaatctc ggagatcaca ggtggcccca ttaacgagac cctcaagctc
     1081 ctcctcagca gcagcgagtg ggtgcagtcg gagaagctgg tgcgggcgct gcagactccg
     1141 ctgatgaggc tgtgcgcctg gtacctgtat ggagagaagc accgcggcta cgcgctgaac
     1201 cccgtggcca acttccacct gcagaacggg gcggtgctgt ggcgcatcaa ctggatggcg
     1261 gatgtgagcc tcagaggcat caccggctcc tgcggcctga tggccaacta ccgctacttc
     1321 ctggaggaga cgggccccaa cagcacctcc tacctcggct ccaagatcat caaagcctct
     1381 gagcaggtcc tcagcctagt ggcccagttt caaaagaact gacagtaaag gtggatacgg
     1441 atccgaa
//
