LOCUS       sequence-49140-e         493 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 493)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MCEE
FEATURES             Location/Qualifiers
     source          1..493
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgc tgggtcgact caaccatgta gccatagcag tgccagattt ggaaaaggct
      121 gcagcatttt ataagaatat tctgggggcc caggtaagtg aagcggtccc tcttcctgaa
      181 catggagtat ctgttgtttt tgtcaacctg ggaaatacca agatggaact gcttcatcca
      241 ttgggacttg acagtccaat tgcaggtttt ctgcagaaaa acaaggctgg aggaatgcat
      301 cacatctgca tcgaggtgga taatattaat gcagctgtga tggatttgaa aaaaaagaag
      361 atccgcagtc taagtgaaga ggtcaaaata ggagcacatg gaaaaccagt gatttttctc
      421 catcctaaag actgtggtgg agtccttgtg gaactggagc aagcttgaca gtaaaggtgg
      481 atacggatcc gaa
//
