LOCUS       sequence-49143-e         957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 957)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: TATDN1
FEATURES             Location/Qualifiers
     source          1..957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             886..906
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             907..924
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             925..948
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgaagttta tcgatattgg tatcaacttg actgacccta tgttcagagg aatttatagg
       61 ggggttcaaa agcatcaaga tgacttacag gatgtaatag ggagagctgt cgagattggt
      121 gttaaaaagt ttatgattac aggtggaaat ctacaagaca gtaaagatgc actgcatttg
      181 gcacaaacaa atggtatgtt tttcagtaca gttggatgtc atcctacaag atgtggtgaa
      241 tttgaaaaga ataaccctga tctttactta aaggagttgc taaatcttgc tgaaaacaat
      301 aaagggaaag ttgtggcaat aggagaatgc ggacttgatt ttgaccgact gcagttttgt
      361 cccaaagata ctcaactcaa atattttgaa aaacagtttg aactgtcaga acaaacaaaa
      421 ttaccaatgt ttcttcattg tcgaaactca catgctgaat ttttggacat aacgaaaaga
      481 aatagagatc ggtgtgtagg gggagtggtg cattcatttg atggtaccaa ggaagcagca
      541 gctgctttga ttgacttgga tctttatata ggatttaatg gttgctcact gaaaactgaa
      601 gctaatttgg aagttttgaa gtcaattcct agtgaaaaat taatgattga gacagatgca
      661 ccttggtgtg gagtcaaaag tacacatgct ggatcaaaat atataagaac tgcatttcct
      721 accaaaaaga agtgggaaag tgggcactgc ttaaaagaca gaaatgaacc ctgccatata
      781 attcaaatat tggagataat gtcagcagtg agagatgagg atccactgga attagccaat
      841 acactatata acaatactat taaagtattt tttcctggaa tagcagagaa cctctacttc
      901 caatcgcacc atcatcacca ccatgattac aaggatgacg acgataagtg aggatcc
//
