LOCUS       sequence-491440-        8535 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes iRFP driven by the CAG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8535)
  AUTHORS   Buenaventura DF, Ghinia-Tegla MG, Emerson MM
  TITLE     Fate-restricted retinal progenitor cells adopt a molecular profile 
            and spatial position distinct from multipotent progenitor cells.
  JOURNAL   Dev Biol. 2018 Nov 1;443(1):35-49. doi: 10.1016/j.ydbio.2018.06.023.
            Epub 2018 Aug 24.
  PUBMED    30145104
REFERENCE   2  (bases 1 to 8535)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ydbio.2018.06.023"; journalName: "Dev Biol"; date: 
            "2018-11-1- 1"; volume: "443"; issue: "1"; pages: "35-49"
COMMENT     Sequence Label: CAG-iRFP
FEATURES             Location/Qualifiers
     source          1..8535
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    47..95
                     /note="synthetic polyadenylation signal"
     misc_feature    109..200
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     149..168
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     enhancer        211..590
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        592..867
                     /label=chicken beta-actin promoter
     intron          868..1884
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1808..1830
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1892..1911
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      2017..2026
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2023..2958
                     /codon_start=1
                     /product="near-infrared fluorescent protein with an 
                     emission peak at 670 nm, engineered from a bacterial 
                     phytochrome (Shcherbakova and Verkhusha, 2013)"
                     /label=iRFP670
                     /note="derived from Rhodopseudomonas palustris RpBphP6"
                     /translation="MARKVDLTSCDREPIHIPGSIQPCGCLLACDAQAVRITRITENAG
                     AFFGRETPRVGELLADYFGETEAHALRNALAQSSDPKRPALIFGWRDGLTGRTFDISLH
                     RHDGTSIIEFEPAAAEQADNPLRLTRQIIARTKELKSLEEMAARVPRYLQAMLGYHRVM
                     LYRFADDGSGMVIGEAKRSDLESFLGQHFPASLVPQQARLLYLKNAIRVVSDSRGISSR
                     IVPEHDASGAALDLSFAHLRSISPCHLEFLRNMGVSASMSLSIIIDGTLWGLIICHHYE
                     PRAVPMAQRVAAEMFADFLSLHFTAAHHQR"
     misc_feature    2980..3549
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2999..3551
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3143..3160)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3370..3389
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3563..5062
                     /codon_start=1
                     /product="secreted alkaline phosphatase from human 
                     placenta"
                     /label=SEAP
                     /note="The natural enzyme has a C-terminal transmembrane 
                     sequence that is replaced by a GPI anchor."
                     /translation="LLLLLLLGLRLQLSLGIIPVEEENPDFWNREAAEALGAAKKLQPA
                     QTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDKH
                     VPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVT
                     TTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKY
                     MFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTHL
                     MGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRA
                     YRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKAR
                     DRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFAR
                     GPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD"
     primer_bind     complement(5557..5576)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5622..5677
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5676..5695)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6038..6054)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6038..6054)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6051..6073)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6062..6078
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6086..6092,6093..6110,6111..6116))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6131..6152
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6211..6406
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6257..6392
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6412..6546
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6449..6468)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6503..6522
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6614..6631)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6785..7373)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6865..6884)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7544..8335,8336..8404))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8167..8186
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8405..8509)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgagaag taatattaag gtacgggagg tacttggagc ggccgcaata aaatatcttt
       61 attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagtactaa catacgctct
      121 ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc
      181 aggtgccaga acatttctct atcgatagtc gacattgatt attgactagt tattaatagt
      241 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      301 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      361 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      421 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
      481 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
      541 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga
      601 gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt
      661 tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg gsgcgcgcca
      721 ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc
      781 aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc
      841 tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc
      901 cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag
      961 gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg
     1021 cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc
     1081 ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc
     1141 tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc
     1201 agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag
     1261 gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg
     1321 tcggtcgggc tgcaaccccc cctgcacccc cctccccgag ttgctgagca cggcccggct
     1381 tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg
     1441 cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg ctcgggggag
     1501 gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc
     1561 cttttatggt aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag
     1621 ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc
     1681 gccggcagga aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt
     1741 ctccctctcc agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc
     1801 agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt
     1861 catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat
     1921 cattttggca aagaattcta tataatggaa gcttgaccgg agccgttagt tgcccgtcgg
     1981 acgcgatttg cgaggcgctt tatgcgcgca ccggtcgcca ccatggcgcg taaggtcgat
     2041 ctcacctcct gcgatcgcga gccgatccac atccccggca gcattcagcc gtgcggctgc
     2101 ctgctagcct gcgacgcgca ggcggtgcgg atcacgcgca ttacggaaaa tgccggcgcg
     2161 ttctttggac gcgaaactcc gcgggtcggt gagctactcg ccgattactt cggcgagacc
     2221 gaagcccatg cgctgcgcaa cgcactggcg cagtcctccg atccaaagcg accggcgctg
     2281 atcttcggtt ggcgcgacgg cctgaccggc cgcaccttcg acatctcact gcatcgccat
     2341 gacggtacat cgatcatcga gttcgagcct gcggcggccg aacaggccga caatccgctg
     2401 cggctgacgc ggcagatcat cgcgcgcacc aaagaactga agtcgctcga agagatggcc
     2461 gcacgggtgc cgcgctatct gcaggcgatg ctcggctatc accgcgtgat gttgtaccgc
     2521 ttcgcggacg acggctccgg gatggtgatc ggcgaggcga agcgcagcga cctcgagagc
     2581 tttctcggtc agcactttcc ggcgtcgctg gtcccgcagc aggcgcggct actgtacttg
     2641 aagaacgcga tccgcgtggt ctcggattcg cgcggcatca gcagccggat cgtgcccgag
     2701 cacgacgcct ccggcgccgc gctcgatctg tcgttcgcgc acctgcgcag catctcgccc
     2761 tgccatctcg aatttctgcg gaacatgggc gtcagcgcct cgatgtcgct gtcgatcatc
     2821 attgacggca cgctatgggg attgatcatc tgtcatcatt acgagccgcg tgccgtgccg
     2881 atggcgcagc gcgtcgcggc cgaaatgttc gccgacttct tatcgctgca cttcaccgcc
     2941 gcccaccacc aacgctaatg tacaagtaaa gcggccaatt ccgccccccc cccccctaac
     3001 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
     3061 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     3121 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     3181 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     3241 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     3301 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     3361 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     3421 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
     3481 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     3541 cgatgataat atggggtcga ggctgctgct gctgctgctg ctgggcctga ggctacagct
     3601 ctccctgggc atcatcccag ttgaggagga gaacccggac ttctggaacc gcgaggcagc
     3661 cgaggccctg ggtgccgcca agaagctgca gcctgcacag acagccgcca agaacctcat
     3721 catcttcctg ggcgatggga tgggggtgtc tacggtgaca gctgccagga tcctaaaagg
     3781 gcagaagaag gacaaactgg ggcctgagat acccctggcc atggaccgct tcccatatgt
     3841 ggctctgtcc aagacataca atgtagacaa acatgtgcca gacagtggag ccacagccac
     3901 ggcctacctg tgcggggtca agggcaactt ccagaccatt ggcttgagtg cagccgcccg
     3961 ctttaaccag tgcaacacga cacgcggcaa cgaggtcatc tccgtgatga atcgggccaa
     4021 gaaagcaggg aagtcagtgg gagtggtaac caccacacga gtgcagcacg cctcgccagc
     4081 cggcacctac gcccacacgg tgaaccgcaa ctggtactcg gacgccgacg tgcctgcctc
     4141 ggcccgccag gaggggtgcc aggacatcgc tacgcagctc atctccaaca tggacattga
     4201 cgtgatccta ggtggaggcc gaaagtacat gtttcgcatg ggaaccccag accctgagta
     4261 cccagatgac tacagccaag gtgggaccag gctggacggg aagaatctgg tgcaggaatg
     4321 gctggcgaag cgccagggtg cccggtatgt gtggaaccgc actgagctca tgcaggcttc
     4381 cctggacccg tctgtgaccc atctcatggg tctctttgag cctggagaca tgaaatacga
     4441 gatccaccga gactccacac tggacccctc cctgatggag atgacagagg ctgccctgcg
     4501 cctgctgagc aggaaccccc gcggcttctt cctcttcgtg gagggtggtc gcatcgacca
     4561 tggtcatcat gaaagcaggg cttaccgggc actgactgag acgatcatgt tcgacgacgc
     4621 cattgagagg gcgggccagc tcaccagcga ggaggacacg ctgagcctcg tcactgccga
     4681 ccactcccac gtcttctcct tcggaggcta ccccctgcga gggagctcca tcttcgggct
     4741 ggcccctggc aaggcccggg acaggaaggc ctacacggtc ctcctatacg gaaacggtcc
     4801 aggctatgtg ctcaaggacg gcgcccggcc ggatgttacc gagagcgaga gcgggagccc
     4861 cgagtatcgg cagcagtcag cagtgcccct ggacgaagag acccacgcag gcgaggacgt
     4921 ggcggtgttc gcgcgcggcc cgcaggcgca cctggttcac ggcgtgcagg agcagacctt
     4981 catagcgcac gtcatggcct tcgccgcctg cctggagccc tacaccgcct gcgacctggc
     5041 gccccccgcc ggcaccaccg acgccgcgca cccggggcgg tccgtggtcc ccgcgttgct
     5101 tcctctgctg gccgggaccc tgctgctgct ggagacggcc actgctccct gagtgtcccg
     5161 tccctggggc tcctgcttcc ccatcccgga gttctcctgc tccccacctc ctgtcgtcct
     5221 gcctggcctc cagcccgagt cgtcatcccc ggagtcccta tacagaggtc ctgccatgga
     5281 accttcccct ccccgtgcgc tctggggact gagcccatga caccaaacct gccccttggc
     5341 tgctctcgga ctccctaccc caaccccagg gactgcaggt tgtgccctgt ggctgcctgc
     5401 accccaggaa aggagggggc tcaggccatc cagccaccac ctacagccca gtggggtcga
     5461 ggatatcgcg gccggccgca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg
     5521 tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta
     5581 tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt
     5641 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
     5701 aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat
     5761 gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc
     5821 tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt
     5881 ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc
     5941 cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag
     6001 atccctcgac ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     6061 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     6121 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     6181 agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa ccatagtccc
     6241 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     6301 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     6361 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc taacttgttt
     6421 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     6481 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     6541 tggatccgct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     6601 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     6661 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     6721 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     6781 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     6841 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     6901 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     6961 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     7021 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     7081 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     7141 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     7201 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     7261 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     7321 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     7381 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     7441 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     7501 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     7561 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7621 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7681 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     7741 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     7801 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     7861 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     7921 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     7981 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     8041 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     8101 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     8161 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     8221 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     8281 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     8341 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     8401 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     8461 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     8521 gaaaagtgcc acctg
//
