LOCUS       sequence-49145-e        1181 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1181)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PHKG2
FEATURES             Location/Qualifiers
     source          1..1181
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             31..201
                     /codon_start=1
                     /product="Z domain of staphylococcal protein A, engineered 
                     to contain multiple positive charges (Hedhammar and Hober, 
                     2007)"
                     /label=Z-basic tag
                     /note="useful for purifying tagged proteins by 
                     cation-exchange chromatography"
                     /translation="VDNKFNKERRRARREIRHLPNLNREQRRAFIRSLRDDPSQSANLL
                     AEAKKLNDAQPK"
     CDS             208..228
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1161..1178
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtggataaca agttcaacaa ggagcgtcga
       61 agagctcgcc gtgaaattcg ccatctgccg aacctgaacc gcgaacagcg tcgcgcattt
      121 attcgcagcc tgcgcgatga tccgagccag agcgcgaacc tgctggcgga agcgaagaag
      181 ctgaacgatg cgcagccgaa gggtaccgag aacctgtact tccaatccat ggggccggag
      241 gatgagctgc ccgactgggc cgccgccaaa gagttttacc agaagtacga ccctaaggac
      301 gtcatcggca gaggagtgag ctctgtggtc cgccgttgtg ttcatcgagc tactggccac
      361 gagtttgcgg tgaagattat ggaagtgaca gctgagcggc tgagtcctga gcagctggag
      421 gaggtgcggg aagccacacg gcgagagaca cacatccttc gccaggtcgc cggccacccc
      481 cacatcatca ccctcatcga ttcctacgag tcttctagct tcatgttcct ggtgtttgac
      541 ctgatgcgga agggagagct gtttgactat ctcacagaga aggtggccct ctctgaaaag
      601 gaaaccaggt ccatcatgcg gtctctgctg gaagcagtga gctttctcca tgccaacaac
      661 attgtgcatc gagatctgaa gcccgagaat attctcctag atgacaatat gcagatccga
      721 ctttcagatt tcgggttctc ctgccacttg gaacctggcg agaagcttcg agagttgtgt
      781 gggaccccag ggtatctagc gccagagatc cttaaatgct ccatggatga aacccaccca
      841 ggctatggca aggaggtcga cctctgggcc tgtggggtga tcttgttcac actcctggct
      901 ggctcgccac ccttctggca ccggcggcag atcctgatgt tacgcatgat catggagggc
      961 cagtaccagt tcagttcccc cgagtgggat gaccgttcca gcactgtcaa agacctgatc
     1021 tccaggctgc tgcaggtgga tcctgaggca cgcctgacag ctgagcaggc cctacagcac
     1081 cccttctttg agcgttgaca gtaaaggtgg atacggatcc gaattcgagc tccgtcgaca
     1141 agcttgcggc cgcactcgag caccaccacc accaccactg a
//
