LOCUS       sequence-491494-       10685 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HA-tagged human CCDC134 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10685)
  AUTHORS   Lampson BL, Ramiotarez AS, Baro M, He L, Hegde M, Koduri V, Pfaff 
            JL, Hanna RE, Kowal J, Shirole NH, He Y, Doench JG, Contessa JN, 
            Locher KP, Kaelin WG Jr
  TITLE     Positive selection CRISPR screens reveal a druggable pocket in an 
            oligosaccharyltransferase required for inflammatory signaling to 
            NF-kappaB.
  JOURNAL   Cell. 2024 Apr 25;187(9):2209-2223.e16. doi: 
            10.1016/j.cell.2024.03.022.
  PUBMED    38670073
REFERENCE   2  (bases 1 to 10685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.03"; journalName: "Cell"; date: "2024-04-25- 
            25"; volume: "187"; issue: "9"; pages: "2209-2223.e16"
COMMENT     Sequence Label: UBC-CCDC134-HA-hygro
FEATURES             Location/Qualifiers
     source          1..10685
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     118..139
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     202..221
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        202..220
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      241..376
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    416..537
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(427..446)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     481..500
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             609..842
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     1031..1047
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1032..1048
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1051..1639
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1157..1168
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1566..1586
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    1695..1728
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(1759..2784)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(1759..2784)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(2802..3301)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2850..2869)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     3243..3266
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             complement(3343..3432)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     protein_bind    3479..3503
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4194..4218)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(4266..5477)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(4410..4429)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     misc_feature    5569..5686
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(5984..6028)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6213..6446
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6943..7068
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7115..7295
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7313..7511)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7338..7358)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7513..7816
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(7991..8579)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8071..8090)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    8908..8955
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     8980..8999
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(9187..9981)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(9187..9981)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9298..9317)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     9908..9927
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(10016..10373)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10030..10165
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        10182..10373
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     10330..10350
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(10375..10479)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      join(10506..10685,1..179)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10593..10612)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
       61 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      121 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      181 gctggccttt tgctcacatg ctaatacgac tcactatagg gcgaattggg tacgtcccta
      241 ggcctccaaa aaagcctcct cactacttct ggaatagctc agaggccgag gcggcctcgg
      301 cctctgcata aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg
      361 gagttagggg cgggatgggc ggagttaggg gcgggatagc tagagccaga catgataaga
      421 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
      481 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttcct
      541 ctcactctct gatattcatt tctttgcaag ttataaatac tgaataataa gatgacatga
      601 actactactg ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc
      661 agagtcacac aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg
      721 cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca
      781 gagagaccca gtacaagcaa aaagcagatc ttgtcttcgt tgggagtgaa ttagcccttc
      841 cagtcccccc ttttctttta aaaagtggct aagatctaca gctgccttgt aagtcattgg
      901 tcttaaaggt acctgaggtg tgactggaaa acccacctcc tcctcctctt gtgcttctag
      961 ccaggcacaa tcagcattgg tagctgctgt attgctactt gtgattgctc catgtttttc
     1021 taggtctcga tcgaggtcga cggtatcgat gcggggaggc ggcccaaagg gagatccgac
     1081 tcgtctgagg gcgaaggcga agacgcggaa gaggccgcag agccggcagc aggccgcggg
     1141 aaggaaggtc cgctggattg agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa
     1201 tccaggtggc aacacaggcg agcagccaag gaaaggacga tgatttcccc gacaacacca
     1261 cggaattgtc agtgcccaac agccgagccc ctgtccagca gcgggcaagg caggcggcga
     1321 tgagttccgc cgtggcaata gggaggggga aagcgaaagt cccggaaagg agctgacagg
     1381 tggtggcaat gccccaacca gtgggggttg cgtcagcaaa cacagtgcac accacgccac
     1441 gttgcctgac aacgggccac aactcctcat aaagagacag caaccaggat ttatacaagg
     1501 aggagaaaat gaaagccata cgggaagcaa tagcatgata caaaggcatt aaagcagcgt
     1561 atccacatag cgtaaaagga gcaacatagt taagaatacc agtcaatctt tcacaaattt
     1621 tgtaatccag aggttgatta tcgataagct tgatatcgaa ttgtacctag tggaaccgga
     1681 acccttaaac atgtataact tcgtataatg tatgctatac gaagttatta ggtccctcga
     1741 cgaattccta cttgtacact attcctttgc cctcggacga gtgctggggc gtcggtttcc
     1801 actatcggcg agtacttcta cacagccatc ggtccagacg gccgcgcttc tgcgggcgat
     1861 ttgtgtacgc ccgacagtcc cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc
     1921 ccaagctgca tcatcgaaat tgccgtcaac caagctctga tagagttggt caagaccaat
     1981 gcggagcata tacgcccgga gccgcggcga tcctgcaagc tccggatgcc tccgctcgaa
     2041 gtagcgcgtc tgctgctcca tacaagccaa ccacggcctc cagaagaaga tgttggcgac
     2101 ctcgtattgg gaatccccga acatcgcctc gctccagtca atgaccgctg ttatgcggcc
     2161 attgtccgtc aggacattgt tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg
     2221 gcagtcctcg gcccaaagca tcagctcatc gagagcctgc gcgacggacg cactgacggt
     2281 gtcgtccatc acagtttgcc agtgatacac atggggatca gcaatcgcgc atatgaaatc
     2341 acgccatgta gtgtattgac cgattccttg cggtccgaat gggccgaacc cgctcgtctg
     2401 gctaagatcg gccgcagcga tcgcatccat ggcctccgcg accggctgca gaacagcggg
     2461 cagttcggtt tcaggcaggt cttgcaacgt gacaccctgt gcacggcggg agatgcaata
     2521 ggtcaggctc tcgctgaatt ccccaatgtc aagcacttcc ggaatcggga gcgcggccga
     2581 tgcaaagtgc cgataaacat aacgatcttt gtagaaacca tcggcgcagc tatttacccg
     2641 caggacatat ccacgccctc ctacatcgaa gctgaaagca cgagattctt cgccctccga
     2701 gagctgcatc aggtcggaga cgctgtcgaa cttttcgatc agaaacttct cgacagacgt
     2761 cgcggtgagt tcaggctttt tcatggtggc gggatgcagg tcgaaaggcc cggagatgag
     2821 gaagaggaga acagcgcggc agacgtgcgc ttttgaagcg tgcagaatgc cgggcctccg
     2881 gaggaccttc gggcgcccgc cccgcccctg agcccgcccc tgagcccgcc cccggaccca
     2941 ccccttccca gcctctgagc ccagaaagcg aaggagcaaa gctgctattg gccgctgccc
     3001 caaaggccta cccgcttcca ttgctcagcg gtgctgtcca tctgcacgag actagtgaga
     3061 cgtgctactt ccatttgtca cgtcctgcac gacgcgagct gcggggcggg ggggaacttc
     3121 ctgactaggg gaggagtaga aggtggcgcg aaggggccac caaagaacgg agccggttgg
     3181 cgcctaccgg tggatgtgga atgtgtgcga ggccagaggc cacttgtgta gcgccaagtg
     3241 cccagcgggg ctgctaaagc gcatgctcca gactgccttg ggaaaagcgc ctcccctacc
     3301 cggtagaatt cgttaacctc gagggccggc cttaattaac taagcgtaat ctggaacgtc
     3361 gtatggatag gatcctgcat agtccgggac atcatacgga tagccggcgt agtcgggcac
     3421 gtcgtagggg taacttccac cgcctccgct tcctccgcca cctgaaccgc ctccacccac
     3481 cactttgtac aagaaagctg ggtttaactc agactgggat ctggagatcc ttgggccttt
     3541 tcggatctcc ttccgcttct cctctttctt tcggcgtttc tcttcttctc tcagggcctt
     3601 ctggaaaggg tcagtgctgg gaatgaactc tgtgcggtcg attttaaatg ggttctggaa
     3661 gttggagtct ttctcactga tccccagctc ctgggccatc aggctgagga tgggcgagtg
     3721 gggcccctgg ttgaagacgc ctgtctggtt gcagaaactg ataccccagc ggatgaggag
     3781 gttccagttg gagttgtggt caaagtaata gtgcacaatc ctcgggaagc gcagcaccac
     3841 atcgccgaag aaggccgtgt tctctaccac gtgggagaaa gcatccttca gcttctcgtc
     3901 ctgggggaag ggcccatctg ggagcacatc agcagcggtg agcactgtcc gggagtcctc
     3961 cagcacctta aagagcccct tgagcatgac atcaaggatc ttgtactgct ggtggatgtc
     4021 gttcagctgt gccaggttct tcagtgccaa cagctgctcc cgccgcttca cctcaaacat
     4081 cttcttgtag atctccaggc ttgggtccag ggaggtcctc aaggtgcctg tggctcccat
     4141 cccagacaaa agcaggacaa agaggaaggc caggaattga agaaggtcca taaagcctgc
     4201 ttttttgtac aaacttgtga ttggccagat ccgaattcgt cgacaattcg atccggcagt
     4261 ctagcgtcta acaaaaaagc caaaaacggc cagaatttag cggacaattt actagtctaa
     4321 cactgaaaat tacatattga cccaaatgat tacatttcaa aaggtgccta aaaaacttca
     4381 caaaacacac tcgccaaccc cgagcgcata gttcaaaacc ggagcttcag ctacttaaga
     4441 agataggtac ataaaaccga ccaaagaaac tgacgcctca cttatccctc ccctcaccag
     4501 aggtccggcg cctgtcgatt caggagagcc taccctaggc ccgaaccctg cgtcctgcga
     4561 cggagaaaag cctaccgcac acctaccggc aggtggcccc accctgcatt ataagccaac
     4621 agaacgggtg acgtcacgac acgacgaggg cgcgcgctcc caaaggtacg ggtgcactgc
     4681 ccaacggcac cgccataact gccgcccccg caacagacga caaaccgagt tctccagtca
     4741 gtgacaaact tcacgtcagg gtccccagat ggtgccccag cccatctcac ccgaataaga
     4801 gctttcccgc attagcgaag gcctcaagac cttgggttct tgccgcccac catgcccccc
     4861 accttgtttc aacgacctca cagcccgcct cacaagcgtc ttccattcaa gactcgggaa
     4921 cagccgccat tttgctgcgc tccccccaac ccccagttca gggcaacctt gctcgcggac
     4981 ccagactaca gcccttggcg gtctctccac acgcttccgt cccaccgagc ggcccggcgg
     5041 ccacgaaagc cccggccagc ccagcagccc gctactcacc aagtgacgat cacagcgatc
     5101 cacaaacaag aaccgcgacc caaatcccgg ctgcgacgga actagctgtg ccacacccgg
     5161 cgcgtcctta tataatcatc ggcgttcacc gccccacgga gatccctccg cagaatcgcc
     5221 gagaagggac tacttttcct cgcctgttcc gctctctgga aagaaaacca gtgccctaga
     5281 gtcacccaag tcccgtccta aaatgtcctt ctgctgatac tggggttcta aggccgagtc
     5341 ttatgagcag cgggccgctg tcctgagcgt ccgggcggaa ggatcaggac gctcgctgcg
     5401 cccttcgtct gacgtggcag cgctcgccgt gaggaggggg gcgcccgcgg gaggcgccaa
     5461 aacccggcgc ggaggccacg cgtcgagcct tctgttgggt taaatctagg gctagagcgg
     5521 gcccggtact aaccaaactg gatctctgct gtccctgtaa taaacccgaa aattttgaat
     5581 ttttgtaatt tgtttttgta attctttagt ttgtatgtct gttgctatta tgtctactat
     5641 tctttcccct gcactgtacc ccccaatccc cccttttctt ttaaaattgt ggatgaatac
     5701 tgccatttgt ctgcagaatt ggcgcacgca gtgccgatcc gttcactaat cgaatggatc
     5761 tgtctctgtc tctctctcca ccttcttctt ctattccttc gggcctgtcg ggtcccctcg
     5821 gggttgggag gtgggtctga aacgataatg gtgaatatcc ctgcctaact ctattcacta
     5881 tagaaagtac agcaaaaact attcttaaac ctaccaagcc tcctactatc attatgaata
     5941 attttatata ccacagccaa tttgttatgt taaaccaatt ccacaaactt gcccatttat
     6001 ctaattccaa taattcttgt tcattctttt cttgctggtt ttgcgattct tcaattaagg
     6061 agtgtattaa gcttgtgtaa ttgttaattt ctctgtccca ctccatccag gtcgtgtgat
     6121 tccaaatctg ttccagagat ttattactcc aactagcatt ccaaggcaca gcagtggtgc
     6181 aaatgagttt tccagagcaa ccccaaatcc ccaggagctg ttgatccttt aggtatcttt
     6241 ccacagccag gattcttgcc tggagctgct tgatgcccca gactgtgagt tgcaacagat
     6301 gctgttgcgc ctcaatagcc ctcagcaaat tgttctgctg ctgcactata ccagacaata
     6361 attgtctggc ctgtaccgtc agcgtcattg acgctgcgcc catagtgctt cctgctgctc
     6421 ccaagaaccc aaggaacaaa gctcctattc ccactgctct tttttctctc tgcaccactc
     6481 ttctctttgc cttggtgggt gctactccta atggttcaat ttttactact ttatatttat
     6541 ataattcact tctccaattg tccctcatat ctcctcctcc aggtctgaag atcagcggcc
     6601 ggccgcttgc tgtgcggtgg tcttactttt gttttgctct tcctctatct tgtctaaagc
     6661 ttccttggtg tcttttatct ctatcctttg atgcacacaa tagagggttg ctactgtatt
     6721 atataatgat ctaagttctt ctgatcctgt ctgaagggat ggttgtagct gtcccagtat
     6781 ttgtctacag ccttctgatg tttctaacag gccaggatta actgcgaatc gttctagctc
     6841 cctgcttgcc catactatat gttttaattt atattttttc tttccccctg gccttaaccg
     6901 aattttttcc catcgcgatc taattctccc ccgcttaata ctgacgctct cgcacccatc
     6961 tctctccttc tagcctccgc tagtcaaaat ttttggcgta ctcaccagtc gccgcccctc
     7021 gcctcttgcc gtgcgcgctt cagcaagccg agtcctgcgt cgagagagct cctctggttt
     7081 ccctttcgct ttcaagtccc tgttcgggcg ccactgctag agattttcca cactgactaa
     7141 aagggtctga gggatctcta gttaccagag tcacacaaca gacgggcaca cactacttga
     7201 agcactcaag gcaagcttta ttgaggctta agcagtgggt tccctagtta gccagagagc
     7261 tcccaggctc agatctggtc taaccagaga gacccagtac aggcaaaacg cgctgcttat
     7321 atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg agttgttacg
     7381 acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga cgtcaatggg
     7441 gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga tgtactgcca
     7501 aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc aagtaggaaa
     7561 gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt cattgacgtc
     7621 aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg cagtttaccg
     7681 taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta tgggaacata
     7741 cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg ggccatttac
     7801 cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc ccgtaattga
     7861 ttactattaa taactaatgc atggcggtaa tacggttatc cacagaatca ggggataacg
     7921 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     7981 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     8041 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     8101 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     8161 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     8221 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     8281 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     8341 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     8401 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     8461 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8521 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8581 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     8641 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     8701 gaagttttaa atcaatctaa agtatatatg agtaacctga ggctatggca gggcctgccg
     8761 ccccgacgtt ggctgcgagc cctgggcctt cacccgaact tggggggtgg ggtggggaaa
     8821 aggaagaaac gcgggcgtat tggccccaat ggggtctcgg tggggtatcg acagagtgcc
     8881 agccctggga ccgaaccccg cgtttatgaa caaacgaccc aacaccgtgc gttttattct
     8941 gtctttttat tgccgtcata gcgcgggttc cttccggtat tgtctccttc cgtgtttcag
     9001 ttagcctccc cctagggtgg gcgaagaact ccagcatgag atccccgcgc tggaggatca
     9061 tccagccggc gtcccggaaa acgattccga agcccaacct ttcatagaag gcggcggtgg
     9121 aatcgaaatc tcgtgatggc aggttgggcg tcgcttggtc ggtcatttcg aaccccagag
     9181 tcccgctcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc
     9241 ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat
     9301 atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc
     9361 gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg
     9421 ggtcacgacg agatcctcgc cgtcgggcat gctcgccttg agcctggcga acagttcggc
     9481 tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat
     9541 ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg
     9601 atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc
     9661 aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc
     9721 cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga
     9781 tagccgcgct gcctcgtctt gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa
     9841 aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt
     9901 ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg
     9961 caatccatct tgttcaatca tgcgaaacga tcctcatcct gtctcttgat cgatctttgc
    10021 aaaagcctag gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgagg
    10081 cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg
    10141 aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg gttgctgact
    10201 aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc
    10261 tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg
    10321 actttccaca ccctaactga cacacattcc acagctggtt ctttccgcct caggactctt
    10381 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    10441 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    10501 acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
    10561 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
    10621 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
    10681 attta
//
