LOCUS       sequence-491507-       10216 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses an NGI-resistant version of STT3A, STT3A E266K, in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10216)
  AUTHORS   Lampson BL, Ramiotarez AS, Baro M, He L, Hegde M, Koduri V, Pfaff 
            JL, Hanna RE, Kowal J, Shirole NH, He Y, Doench JG, Contessa JN, 
            Locher KP, Kaelin WG Jr
  TITLE     Positive selection CRISPR screens reveal a druggable pocket in an 
            oligosaccharyltransferase required for inflammatory signaling to 
            NF-kappaB.
  JOURNAL   Cell. 2024 Apr 25;187(9):2209-2223.e16. doi: 
            10.1016/j.cell.2024.03.022.
  PUBMED    38670073
REFERENCE   2  (bases 1 to 10216)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.03"; journalName: "Cell"; date: "2024-04-25- 
            25"; volume: "187"; issue: "9"; pages: "2209-2223.e16"
COMMENT     Sequence Label: STT3A E266K
FEATURES             Location/Qualifiers
     source          1..10216
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(749..765)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(749..765)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(762..784)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    773..789
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(797..803,804..821,822..827))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    842..863
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(980..997)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1151..1739)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1231..1250)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1910..2701,2702..2770))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2533..2552
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2771..2875)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    2923..3057
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2960..2979)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3014..3033
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             3225..3858
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3905..4030
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4527..4760
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4945..4989
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5252..5367
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        5425..5728
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5729..5932
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5883..5903
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5929..5953
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        8275..8774
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8310..8333)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8707..8726
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8795..9394
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8795..8814)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9291..9311
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9408..9996
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9461..9481)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9879..9890)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             join(10203..10216,1..620)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
       61 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
      121 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
      181 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
      241 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
      301 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
      361 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
      421 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
      481 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
      541 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
      601 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
      661 tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt ttaccgtcga
      721 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
      781 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
      841 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
      901 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
      961 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     1021 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     1081 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     1141 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     1201 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     1261 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     1321 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     1381 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1441 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     1501 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     1561 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     1621 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     1681 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     1741 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     1801 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     1861 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     1921 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     1981 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     2041 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     2101 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     2161 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     2221 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     2281 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     2341 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     2401 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     2461 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     2521 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     2581 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     2641 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     2701 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     2761 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     2821 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     2881 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt tattgcagct
     2941 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     3001 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
     3061 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
     3121 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt
     3181 taaagaaatt gtatttgtta aatatgtact acaaacttag tagttggaag ggctaattca
     3241 ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc
     3301 tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg
     3361 ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac
     3421 cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga
     3481 gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta
     3541 cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg
     3601 gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc
     3661 tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     3721 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     3781 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     3841 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
     3901 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
     3961 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
     4021 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
     4081 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
     4141 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
     4201 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
     4261 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
     4321 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg
     4381 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     4441 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     4501 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     4561 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     4621 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     4681 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     4741 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     4801 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     4861 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     4921 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     4981 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     5041 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     5101 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
     5161 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
     5221 cgattagtga acggatctcg acggtatcgc ctttaaaaga aaagggggga ttggggggta
     5281 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     5341 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt
     5401 ttatcgataa gcttgggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     5461 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     5521 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     5581 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     5641 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     5701 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
     5761 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
     5821 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
     5881 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt
     5941 gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg
     6001 actctactag aggatcgcta gcgctaccgg actcagatct cgagctcatc gggatccgcc
     6061 accatgacta agtttggatt tttgcgattg tcctatgaga agcaggacac acttttgaag
     6121 cttctcattc tgtcaatggc tgctgtatta tccttctcca ctcgtctgtt tgctgtcctg
     6181 agatttgaaa gtgttatcca tgagtttgat ccgtacttta attatcggac taccaggttc
     6241 ctggctgagg aggggtttta taaattccat aactggtttg atgaccgagc ctggtaccct
     6301 ttgggacgaa tcattggagg aacaatttac ccaggtttaa tgatcacctc tgctgcaatc
     6361 taccatgtac tccatttttt ccacatcacc atcgacattc ggaatgtctg tgtgttcctg
     6421 gcccctctct tctcctcctt cacaacgatc gtcacgtacc accttaccaa agagctcaag
     6481 gatgcagggg ctgggcttct tgctgctgcc atgattgctg tagttcctgg atatatctcc
     6541 cgatctgtgg ctggctccta tgataatgaa gggattgcca tcttttgcat gctactcacc
     6601 tactacatgt ggatcaaggc agtaaagact ggttccatct gttgggcagc taagtgtgcc
     6661 cttgcttatt tctacatggt ctcgtcatgg ggaggttatg tgttcctgat caacttaatt
     6721 cctctccacg tcctcgtgct gatgctcaca ggccgtttct ctcaccggat ctatgtggcc
     6781 tactgtactg tttactgcct gggcactata ctttctatgc agatctcctt tgtgggtttc
     6841 cagcctgtcc tttcatcaaa gcacatggca gcctttgggg tctttggtct ctgccagatc
     6901 catgcctttg tggattacct gcgcagcaag ttgaatccac aacaatttga agttcttttc
     6961 cggagcgtca tctctctggt aggctttgtc cttctcaccg tgggagctct cctcatgctg
     7021 acaggaaaaa tatctccctg gacggggcgt ttctactcgc tgctagatcc ctcttatgct
     7081 aagaacaaca tccccatcat tgcttctgtg tctgagcatc agcccacaac ctggtcctca
     7141 tactattttg acctgcagct actcgtcttc atgtttccag ttggtctgta ttactgcttt
     7201 agcaacctgt ctgatgcccg gatttttatc atcatgtatg gtgtgaccag catgtacttt
     7261 tcagctgtaa tggtgcgtct aatgctagtg ttggcacctg ttatgtgcat tctctctggc
     7321 attggagtct cccaggtgct gtccacatac atgaagaatc tggacataag tcgtccagac
     7381 aagaagagca agaagcaaca ggattccacc taccctatta agaatgaagt ggcaagtggg
     7441 atgatactgg tcatggcttt ctttctcatc acctacacct ttcattcaac ctgggtgacc
     7501 agtgaggcct actcttctcc gtccattgta ctatctgccc gtggtgggga tggcagtagg
     7561 atcatatttg atgacttccg agaagcatat tattggcttc gtcataatac tccagaggat
     7621 gcgaaggtca tgtcctggtg ggattatggc tatcagatta cagctatggc aaaccgaaca
     7681 attttagtgg acaataacac atggaataat acccatattt ctcgagtagg gcaggcaatg
     7741 gcgtccacag aggaaaaagc ctatgagatc atgagggagc tcgatgtcag ctatgtgctg
     7801 gtcatttttg gaggcctcac tgggtattcc tctgatgata tcaacaagtt tctttggatg
     7861 gtccggattg gagggagcac agatacaggc aaacatatca aggagaatga ctattatact
     7921 ccaactgggg agttccgtgt ggaccgtgaa ggttctccag tgctgctcaa ctgcctcatg
     7981 tacaagatgt gttactatcg ctttggacag gtttacacag aagccaagcg tcctccaggc
     8041 tttgaccgtg tccgaaatgc tgagattggg aataaagact ttgagcttga tgtcctggag
     8101 gaagcatata ccacagaaca ttggctggtc aggatataca aggtaaagga cctggataat
     8161 cgaggcttgt caaggacagg ctcgggcagc ggtggctctg gaggttctgt gagcggctgg
     8221 cggctgttca agaagattag ctaagaattc cgacatgctc tagataattc taccgggtag
     8281 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt
     8341 ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac
     8401 cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt
     8461 tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca
     8521 ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca
     8581 gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt
     8641 ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag
     8701 gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat
     8761 ctccgggcct ttcgacctgc agcccaagct taccatgacc gagtacaagc ccacggtgcg
     8821 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     8881 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     8941 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     9001 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     9061 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     9121 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     9181 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     9241 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     9301 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     9361 cacctggtgc atgacccgca agcccggtgc ctgaccgcgt ctggaacaat caacctctgg
     9421 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     9481 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     9541 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     9601 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     9661 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     9721 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     9781 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
     9841 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     9901 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     9961 agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattaattct gcagtcgaga
    10021 cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc
    10081 ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt
    10141 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagagggg
    10201 actggaaggg ctaatt
//
