LOCUS       sequence-49152-e         688 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 688)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ASPH
FEATURES             Location/Qualifiers
     source          1..688
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgc gctcactcta caatgtgaat ggactgaaag cacagccttg gtggacccca
      121 aaagaaacgg gctacacaga gttagtaaag tctttagaaa gaaactggaa gttaatccga
      181 gatgaaggcc ttgcagtgat ggataaagcc aaaggtctct tcctgcctga ggatgaaaac
      241 ctgagggaaa aaggggactg gagccagttc acgctgtggc agcaaggaag aagaaatgaa
      301 aatgcctgca aaggagctcc taaaacctgt accttactag aaaagttccc cgagacaaca
      361 ggatgcagaa gaggacagat caaatattcc atcatgcacc ccgggactca cgtgtggccg
      421 cacacagggc ccacaaactg caggctccga atgcacctgg gcttggtgat tcccaaggaa
      481 ggctgcaaga ttcgatgtgc caacgagacc aagacctggg aggaaggcaa ggtgctcatc
      541 tttgatgact cctttgagca cgaggtatgg caggatgcct catctttccg gctgatattc
      601 atcgtggatg tgtggcatcc ggaactgaca ccacagcaga gacgcagcct tccagcaatt
      661 tgacagtaaa ggtggatacg gatccgaa
//
