LOCUS       sequence-491522-        8785 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ZFa Lentivirus expressing VP16-ZF37-2A-TagBFP from the EF1a 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8785)
  AUTHORS   Kabaria SR, Bae Y, Ehmann ME, Lende-Dorn BA, Beitz AM, Peterman EL, 
            Love KS, Ploessl DS, Galloway KE
  TITLE     Programmable promoter editing for precise control of transgene 
            expression.
  JOURNAL   Nat Biotechnol. 2025 Oct 13. doi: 10.1038/s41587-025-02854-y.
  PUBMED    41083901
REFERENCE   2  (bases 1 to 8785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2025 Oct 13. doi: 10.1038/s41587-025-02854-y."
COMMENT     Sequence Label: LentiX_EF1a-VP16-ZF37-2A-TagBFP-WPRE_pKG3675
FEATURES             Location/Qualifiers
     source          1..8785
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             88..321
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    393..514
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(430..449)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     484..503
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      554..689
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(709..728)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(710..728)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(738..755)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(738..754)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(747..769)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      896..1351
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(983..1002)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1193..1214
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1377..1481
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1482..1550,1551..2342)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1700..1719)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2513..3101
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3002..3021
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3255..3272
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3389..3410
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3425..3430,3431..3448,3449..3455)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3463..3479
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3468..3490
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3487..3503
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3487..3503
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3522..3542
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3524..3542
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3570..3796
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3797..3977
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4024..4149
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4642..4875
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5060..5104
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5371..5487
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5514..6695
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5744..6686
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6643..6663
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     6697..6721
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      6758..6767
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6767..6832
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6839..6859
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7412..7468
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7481..8182
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    8193..8781
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8246..8266)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8664..8675)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cgctgatata gggcccggta cctttaagac caatgactta caaggcagct gtagatctta
       61 gccacttttt aaaagaaaag gggggactgg aagggctagc tcactcccaa cgaagacaag
      121 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
      181 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
      241 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
      301 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
      361 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
      421 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
      481 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
      541 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
      601 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
      661 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
      721 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
      781 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
      841 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
      901 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
      961 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     1021 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     1081 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     1141 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     1201 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     1261 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     1321 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     1381 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     1441 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     1501 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     1561 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     1621 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     1681 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     1741 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     1801 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     1861 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     1921 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     1981 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     2041 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     2101 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     2161 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     2221 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     2281 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     2341 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     2401 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     2461 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     2521 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     2581 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     2641 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     2701 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     2761 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     2821 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     2881 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     2941 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3001 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3061 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3121 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     3181 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     3241 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     3301 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     3361 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     3421 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     3481 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
     3541 gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg
     3601 caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg
     3661 ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct
     3721 gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct
     3781 agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     3841 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     3901 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     3961 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     4021 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     4081 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     4141 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     4201 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     4261 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     4321 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     4381 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     4441 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt
     4501 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     4561 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     4621 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     4681 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     4741 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     4801 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     4861 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     4921 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     4981 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     5041 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     5101 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     5161 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     5221 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
     5281 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
     5341 agtgaacgga tctcgacggt atcggttaac ttttaaaaga aaagggggga ttggggggta
     5401 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     5461 aaaacaaatt acaaaattca aaattttatc gtccagtgtg gtggctactt actgctccgg
     5521 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
     5581 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
     5641 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
     5701 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
     5761 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
     5821 gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga
     5881 gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcttg
     5941 ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg
     6001 ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag
     6061 atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg
     6121 gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc
     6181 ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc
     6241 tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg
     6301 cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc
     6361 ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct
     6421 cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt
     6481 tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt
     6541 ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct
     6601 ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg
     6661 ttcaaagttt ttttcttcca tttcaggtgt cgtgagagct cgtttagtga accgtcagat
     6721 cgcctggaga cgccatccac gctgtaatgg agccaccgcc accatggatt ataaggatca
     6781 cgatggcgac tacaaggacc acgatattga ctacaaagac gatgacgata aaatggctcc
     6841 taagaaaaag cgcaaagtcg gtatccatgg cgttcccgtc gacggtggac tgcaggcccc
     6901 accaaccgat gtttctttag gtgatgagtt acatttggac ggtgaggacg tagctatggc
     6961 acatgcagat gccttagacg attttgactt ggatatgtta ggagatggag attcaccagg
     7021 acctggtttc acaccacacg attcagctcc atacggtgct ttggacatgg ccgatttcga
     7081 atttgaacag atgtttactg atgccttggg tatagacgaa tacggtggtg gagctagacc
     7141 cggggagcgc cccttccagt gtcgcatttg catgcggaac ttttcgagaa atttcatttt
     7201 gcaaagacat acccgtactc ataccggtga aaaaccgttt cagtgtcgga tctgtatgcg
     7261 aaatttctcc gatagagcta atttgaggag gcatctgcgt acgcacaccg gcgagaagcc
     7321 attccaatgc cgaatatgca tgcgcaactt cagtagacat gatcaattga ctagacacct
     7381 aaaaacccac ctgagaggat ctggaagcgg agctactaac ttcagcctgc tgaagcaggc
     7441 tggagacgtg gaggagaacc ctggacctgc accaggaagt atgagcgagc tgattaagga
     7501 gaacatgcac atgaagctgt acatggaggg caccgtggac aaccatcact tcaagtgcac
     7561 atccgagggc gaaggcaagc cctacgaggg cacccagacc atgagaatca aggtggtcga
     7621 gggcggccct ctccccttcg ccttcgacat cctggctact agcttcctct acggcagcaa
     7681 gaccttcatc aaccacaccc agggcatccc cgacttcttc aagcagtcct tccctgaggg
     7741 cttcacatgg gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga
     7801 caccagcctc caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttcac
     7861 atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggccttca ccgagacgct
     7921 gtaccccgct gacggcggcc tggaaggcag aaacgacatg gccctgaagc tcgtgggcgg
     7981 gagccatctg atcgcaaaca tcaagaccac atatagatcc aagaaacccg ctaagaacct
     8041 caagatgcct ggcgtctact atgtggacta cagactggaa agaatcaagg aggccaacaa
     8101 cgaaacgtac gtcgagcagc acgaggtggc agtggccaga tactgcgacc tccctagcaa
     8161 actggggcac aagcttaatt aacaacgccg ataatcaacc tctggattac aaaatttgtg
     8221 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     8281 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     8341 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     8401 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     8461 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     8521 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     8581 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     8641 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     8701 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     8761 ccctttgggc cgcctccccg ccgct
//
