LOCUS       sequence-491535-        5847 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Cre recombinase under the control of the Nppa Promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5847)
  AUTHORS   Sweat ME, Cao Y, Zhang X, Burnicka-Turek O, Perez-Cervantes C, 
            Arulsamy K, Lu F, Keating EM, Akerberg BN, Ma Q, Wakimoto H, Gorham 
            JM, Hill LD, Kyoung Song M, Trembley MA, Wang P, Gianeselli M, 
            Prondzynski M, Bortolin RH, Bezzerides VJ, Chen K, Seidman JG, 
            Seidman CE, Moskowitz IP, Pu WT
  TITLE     Tbx5 maintains atrial identity in post-natal cardiomyocytes by 
            regulating an atrial-specific enhancer network.
  JOURNAL   Nat Cardiovasc Res. 2023 Oct;2(10):881-898. doi: 
            10.1038/s44161-023-00334-7. Epub 2023 Oct 12.
  PUBMED    38344303
REFERENCE   2  (bases 1 to 5847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44161-023-00334-7"; journalName: "Nat Cardiovasc Res"; 
            date: "2023-10"; volume: "2"; issue: "10"; pages: "881-898"
COMMENT     Sequence Label: AAV-Nppa-Cre
FEATURES             Location/Qualifiers
     source          1..5847
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(175..966,967..1035))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     798..817
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1036..1140)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1166..1621)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1303..1324)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1515..1534
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1748..1770
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1762..1779
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1763..1779
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   1798..1927
                     /label=AAV2 ITR
     polyA_signal    2020..2075
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     2121..2140
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(2155..2868)
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             complement(2883..2939)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(3978..3998)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4861..4877)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4861..4877)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4874..4896)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4885..4901
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4909..4915,4916..4933,4934..4939))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4954..4975
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(join(5263..5847,1..4))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5343..5362)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
       61 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
      121 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
      181 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
      241 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
      301 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
      361 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
      421 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
      481 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
      541 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
      601 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
      661 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
      721 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
      781 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
      841 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
      901 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
      961 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     1021 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     1081 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     1141 cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg
     1201 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     1261 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     1321 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     1381 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     1441 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     1501 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     1561 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     1621 tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     1681 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
     1741 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattaggtta attaaggctg
     1801 cgcgctcgct cgctcactga ggccgcccgg gcaaagcccg ggcgtcgggc gacctttggt
     1861 cgcccggcct cagtgagcga gcgagcgcgc agagagggag tggccaactc catcactagg
     1921 ggttccttgt agttaatgat taacccgcca tgctacttat ctaccagggt aatgggtatt
     1981 atgggtggtc gaaattcaga tcaacgaatt gcttccgagt gagagacaca aaaaattcca
     2041 acacactatt gcaatgaaaa taaatttcct ttattagcca gaagtcagat gctcaagggg
     2101 cttcatgatg tccccataat ttttggcaga gggaaaaaga tcggatccgg tacctcaatt
     2161 aagtttgtgc cccagtttgc tagggaggtc gcagtatctg gccacagcca cctcgtgctg
     2221 ctcgacgtag gtctctttgt cggcctcctt gattctttcc agtctgtggt ccacatagta
     2281 gacgccgggc atcttgaggt tcttagcggg tttcttggat ctgtatgtgg tcttgaagtt
     2341 gcagatcagg tggcccccgc ccacgagctt cagggccatg tcgcttctgc cttccaggcc
     2401 gccgtcagcg gggtacagca tctcggtgtt ggcctcccag ccgagtgttt tcttctgcat
     2461 cacagggccg ttggatggga agttcacccc tctgatcttg acgttgtaga tgaggcagcc
     2521 gtcctggagg ctggtgtcct gggtagcggt cagcacgccc ccgtcttcgt atgtggtgac
     2581 tctctcccat gtgaagccct cagggaagga ctgcttaaag aagtcgggga tgccctgggt
     2641 gtggttgatg aaggttctgc tgccgtacat gaagctggta gccaggatgt cgaaggcgaa
     2701 ggggagaggg ccgccctcga ccaccttgat tctcatggtc tgggtgccct cgtagggctt
     2761 gccttcgccc tcggatgtgc acttgaagtg gtggttgttc acggtgccct ccatgtacag
     2821 cttcatgtgc atgttctcct taatcagctc ttcgccctta gacaccatgc ggccgcctcg
     2881 agggggccgg ggttctcctc cacgtcgccg gcctgcttca gcagggagaa gttggtggcg
     2941 tcaccatctt cgagcagtct caccatggcc ccggtttcag agtcaagatt gcggatatag
     3001 ttcataacga tgttcacatt tgtccaacct ccagcttgca taatctctgg tatggagact
     3061 cctgcccgcg ccatgtctcg tgcggccccc accctagcag aatgacctga ccaggcgaga
     3121 tacctctggc cactgtcatc cttggctccg tatatgagtc ggtgggttgc ctcaaagatc
     3181 ccttcaaggg cccgtgtact caattggctg gtggcagagg gcgcggcgac tccgtttttt
     3241 ctcactcgac agaacaagta attattaggg tcgtcagcca cgccggacac tgagatccat
     3301 ctttccacca gcttcgttac gcccagggac aaggcctttt caacgccagc ggtgctgacc
     3361 aatgtctttg tgcgtccaat atgaataagc atccggccgc cgtccgttcg actgatgtct
     3421 ttcactctga tccgggcgat ctcagctatg cgcagaagtg tgttatatgc aattcccaga
     3481 aatgcgaggt tcctgatgtc ctggcaccga tcgctgttct ccatcaggga tctgacctgg
     3541 tcgaaatcag tccgctcaaa agcgagagcc tgcttagccc tctcacccgc atctacgttt
     3601 tcctttctaa ttctcctcat aacaagggag actgcgtttg aatcgctagg ccgggggagc
     3661 ccgctgcgtc gatgcagcat attcagttgg cccaagtgct gctggatagt cttgacagcc
     3721 agccctcttg cctggagata cagcaggtaa tcgcgcacgt cctccggctc ggcggggaac
     3781 catttgcgat tgttcagctt acaccaggct gcccaggatc ggcacacgct aagcagcatt
     3841 ttccaggtgt gttcagaaaa cgcctgtcta tccctaaaca tatccatgag atttttcctg
     3901 acctcatcgc tagtggcatc tactggcagc gcagggaggt tctggtgaac agtcaggaga
     3961 ttggatctag accatggcac cttcctcttc ttcttgggca tggtgtctcc tagctgccag
     4021 catctcccgt ttttatagcc ccccagctct ttgagaaggc aggggggcta gaaccctccc
     4081 cattctgtca cttgcagcga taaagcgatg gagtgaggag ccagcagaag atgccctttt
     4141 aaagttatca gttcggcaaa gcttgtcacg gcccctttgc cttgagcagc tccactcacc
     4201 cccctccccc agaccctcag ctgcaagagt cacattcttg ctgatttgcc tcaagaggcc
     4261 cccacttcaa aggtgtgaga ggagcagaaa agagtccttg gttatcttcc tgggcgctcg
     4321 cctcaggatt ctttctccct ggcaacagga cccatgcagg gaagcagagg gtgacggctg
     4381 gcctgcaccc agctccccct gcttgctgct cagctgtcag gggctccaaa taagggcggg
     4441 agaggaggca atgacaggca gctgggaggc cagctaggct ggccagggag tacacatgct
     4501 ggagctagct tcacacctga ttcatcggcc tcgtgggtgg acctctggcc ccagacagcc
     4561 cacccccacc ctagatgtcc ctgttagcag tcaggtgaaa gagctattat ttttttttta
     4621 gagtatgaag tcagccagga agccaacatg atacagggcc cggatcccca ttaccctggt
     4681 agataagtag catggcgggt taatcattaa ctacaaggaa cccctagtga tggagttggc
     4741 cactccctct ctgcgcgctc gctcgctcac tgaggccgcc cgggctttgc ccgggcggcc
     4801 tcagtgagcg agcgagcgcg cagcctaatt aaggccttaa ttaaatctgg cgtaatcatg
     4861 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     4921 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     4981 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     5041 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     5101 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     5161 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     5221 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     5281 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     5341 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     5401 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     5461 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     5521 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     5581 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     5641 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     5701 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     5761 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     5821 gcagattacg cgcagaaaaa aaggatc
//
