LOCUS       sequence-491542-       12668 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of ER-specific SUNTAG reporter: piggyBac vector
            expressing cytERM-SUNTAG MS2; 5' UTR includes cricket paralysis 
            virus IRES, allowing escape from translation initiation regulation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12668)
  AUTHORS   Choi H, Liao YC, Yoon YJ, Grimm J, Wang N, Lavis LD, Singer RH, 
            Lippincott-Schwartz J
  TITLE     Secretome translation shaped by lysosomes and lunapark-marked ER 
            junctions.
  JOURNAL   Nature. 2025 Nov 5. doi: 10.1038/s41586-025-09718-0.
  PUBMED    41193816
REFERENCE   2  (bases 1 to 12668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2025 Nov 5. doi: 10.1038/s41586-025-09718-0."
COMMENT     Sequence Label: pPB-CrPV-IRES-cytERM-SUNTAG-MS2
FEATURES             Location/Qualifiers
     source          1..12668
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1162..1617)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1299..1320)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1511..1530
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1744..1766
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1758..1775
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1759..1775
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   1905..1967
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    2420..2438
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2456..2474
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2492..2510
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2528..2546
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2564..2582
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2600..2618
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2636..2654
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2699..2723
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3174..3872
                     /codon_start=1
                     /product="far-red monomeric fluorescent protein with an 
                     emission peak at 657 nm (Morozova et al., 2010)"
                     /label=TagRFP657
                     /note="derived from mKate"
                     /translation="MSELITENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTQRIKV
                     VEGGPLPFAFDILATSFMYGSHTFINHTQGIPDFWKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFLSNGPVMQKKTLGWEAHTEMLYPADGGLEGRTALALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDYRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             3912..3968
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3984..4040
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4056..4112
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4128..4184
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4200..4256
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4272..4328
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4344..4400
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4416..4472
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4488..4544
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4560..4616
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4632..4688
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4704..4760
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4776..4832
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4848..4904
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4920..4976
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4992..5048
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5064..5120
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5136..5192
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5208..5264
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5280..5336
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5352..5408
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5424..5480
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5568..5624
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             join(5676..5678,5679..5681,5682..6389)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFISTTGKLPVPWPTLVTTLSHGVQVFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELY"
     primer_bind     complement(5721..5742)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             7886..7927
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7944..7961
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8018..8035)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8024..8231
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        8308..8519
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             8532..8800
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             8834..9577
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             9578..9631
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             9632..10231
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9632..9651)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10128..10148
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    10270..10391
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10307..10326)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10361..10380
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   11153..11187
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(11678..11694)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11678..11694)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11691..11713)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11702..11718
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11726..11732,11733..11750,11751..11756))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11771..11792
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11909..11926)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12080..12668)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12160..12179)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     1201 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     1261 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     1321 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     1381 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     1441 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     1501 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     1561 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
     1621 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     1681 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     1741 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcctcgttc attcacgttt
     1801 ttgaacccgt ggaggacggg cagactcgcg gtgcaaatgt gttttacagc gtgatggagc
     1861 agatgaagat gctcgacacg ctgcagaaca cgcagctaga ttaaccctag aaagataatc
     1921 atattgtgac gtacgttaaa gataatcatg tgtaaaattg acgcatgtgt tttatcggtc
     1981 tgtatatcga ggtttattta ttaatttgaa tagatattaa gttttattat atttacactt
     2041 acatactaat aataaattca acaaacaatt tatttatgtt tatttattta ttaaaaaaaa
     2101 caaaaactca aaatttcttc tataaagtaa caaaactttt atgagggaca gccccccccc
     2161 aaagccccca gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg
     2221 gggctccgct ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag
     2281 cccgggcacg gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt
     2341 gagcctgcag acacctgggg ggatacgggg aaaaggcctc cacggccact agtctttcgt
     2401 cttcaagaat tccgtttact ccctatcagt gatagagaac gtatgaagag tttactccct
     2461 atcagtgata gagaacgtat gcagacttta ctccctatca gtgatagaga acgtataagg
     2521 agtttactcc ctatcagtga tagagaacgt atgaccagtt tactccctat cagtgataga
     2581 gaacgtatct acagtttact ccctatcagt gatagagaac gtatatccag tttactccct
     2641 atcagtgata gagaacgtat aagctttagg cgtgtacggt gggcgcctat aaaagcagag
     2701 ctcgtttagt gaaccgtcag atcgcctgga gcaattccac aacacttttg tcttatacca
     2761 actttccgta ccacttccta ccctcgtaaa ggtctagagc tagcatcgct gtgatcgtca
     2821 cttggaaagc aaaaatgtga tcttgcttgt aaatacaatt ttgagaggtt aataaattac
     2881 aagtagtgct atttttgtat ttaggttagc tatttagctt tacgttccag gatgcctagt
     2941 ggcagcccca caatatccag gaagccctct ctgcggtttt tcagattagg tagtcgaaaa
     3001 acctaagaaa tttacctgct acatttcaag ataccatgga tcctgtggtg gtgctggggc
     3061 tctgtctctc ctgtttgctt ctcctttcac tctggaaaca gagctatggg ggagggaagc
     3121 ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc accggtcgcc accatgagcg
     3181 agctgatcac cgagaacatg cacatgaagc tgtacatgga gggcaccgtg aacaaccacc
     3241 acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag acccagagaa
     3301 tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct accagcttca
     3361 tgtacggcag tcacaccttc atcaaccaca cccagggcat ccccgacttc tggaagcagt
     3421 ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctga
     3481 ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aagatcagag
     3541 gggtgaactt cctatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggccc
     3601 acaccgagat gctgtacccc gctgacggcg gcctggaagg cagaaccgcg ctggccctga
     3661 agctcgtggg cgggggccac ctgatctgca acttcaagac cacatacaga tccaagaaac
     3721 ccgctaagaa cctcaagatg cccggcgtct actatgtgga ctacagactg gaaagaatca
     3781 aggaggccga caaagagacc tacgtcgagc agcacgaggt ggctgtggcc agatactgcg
     3841 acctccctag caaactgggg cacaaactta atggcggacc gggtggatct ggaggtggag
     3901 gttctggagg agaagaactt ttgagcaaga attatcatct tgagaacgaa gtggctcgtc
     3961 ttaagaaagg ttctggcagt ggagaagaac tgctttcaaa gaattaccac ctggaaaatg
     4021 aggtagctag actgaaaaag gggagcggaa gtggggagga gttgctgagc aaaaattatc
     4081 atttggagaa cgaagtagca cgactaaaga aagggtccgg atcgggtgag gagttactct
     4141 cgaaaaatta tcatctcgaa aacgaagtgg ctcggctaaa aaagggcagt ggttctggag
     4201 aagagctatt atctaaaaac taccacctcg aaaatgaggt ggcacgctta aaaaagggaa
     4261 gtggcagtgg tgaagagcta ctatccaaga attatcatct tgagaacgag gtagcgcgtt
     4321 tgaagaaggg ttccggctca ggagaggaac tgctctcgaa gaactatcat cttgaaaatg
     4381 aggtcgctcg attaaaaaag ggatcgggca gtggtgagga actactttca aagaattacc
     4441 acctcgaaaa cgaagtagct cgattaaaga aaggttcagg gtcgggtgaa gaattactga
     4501 gtaaaaatta tcatctggaa aatgaggtag cgagactaaa aaaggggagt ggttctggcg
     4561 aagagttgct atcgaaaaat tatcatcttg agaacgaagt tgctaggctc aaaaagggct
     4621 caggctcagg cgaggagttg ctctcgaaaa actaccactt ggaaaatgag gtcgcgaggt
     4681 tgaaaaaggg gagcgggtcg ggcgaggagt tattgagcaa aaactatcat ttagagaacg
     4741 aagtcgcgcg cttaaagaaa ggctcgggct cgggcgaaga actcttatcg aagaactacc
     4801 acctcgaaaa tgaggtcgcc aggttgaaaa agggcagtgg cagcggggag gaactcttga
     4861 gcaagaacta ccacttggag aatgaggtcg cgagattgaa gaaagggtcg gggagcggcg
     4921 aggaattgct cagcaagaat tatcatttgg agaacgaagt cgccaggctc aagaaaggct
     4981 cggggtcggg ggaggaattg ttgagtaaaa actaccactt ggaaaatgaa gtcgccaggc
     5041 tcaaaaaagg gagtgggagc ggcgaagagt tattgagcaa aaattaccac ttggagaacg
     5101 aagtggcaag gctcaagaaa gggagcggca gcggggagga gctcttatcg aagaactacc
     5161 acttagagaa tgaagtcgcc cgcttgaaga aaggctcggg gagcggggaa gagctcttga
     5221 gcaagaacta ccacttggaa aatgaggtgg cgcgcttgaa gaaagggagc gggagcgggg
     5281 aagagttact atctaagaat tatcatctcg agaacgaggt ggctcgacta aagaagggct
     5341 ccggcagtgg ggaggaactc ctgtcgaaga actatcatct tgaaaatgag gttgcaagac
     5401 ttaaaaaggg gtccggatca ggtgaggaac tactcagtaa gaattaccac ctggaaaacg
     5461 aagttgcacg tttgaagaaa ggatcaggat caggcgaaga actgctctca aaagattatc
     5521 atttggaaaa tgaggttgca cgtttaaaaa agggaagtgg cagtggtgag gaacttctgt
     5581 cgaaaaatta tcatctcgag aatgaagtag cccgacttaa aaagggttct ggctcgggtg
     5641 gaggcggtgg atcaggtgga ggaggttcac tgcagatggt gagcaagggc gaggagctgt
     5701 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     5761 gcgtgagggg cgagggcgag ggcgatgcca ccaacggcaa gctgaccctg aagttcatca
     5821 gtactaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg agccacggcg
     5881 tgcaggtgtt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     5941 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc acctacaaga
     6001 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggcg
     6061 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac ttcaacagcc
     6121 acaacatcta tatcatggcc gtcaagcaga agaacggcat caaggccaac ttcaagatcc
     6181 gccacaacgt ggaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     6241 tcggcgacgg ccccgtgctg ctgcccgaca gccactacct gagcacccag tccgtgctga
     6301 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttccgc accgccgccg
     6361 ggatcactct cggcatggac gagctgtaca ttgttttgtt tttttttttt ttggttttgt
     6421 ctttttttaa tagtcattcc aagtatccat gaaataagtg gttacaggaa gtccctcacc
     6481 ctcccaaaag ccacccccac tcctaagagg aggatggtcg cgtccatgcc ctgagtccac
     6541 cccggggaag gtgacaaccg gtaacctaca aacgggtgga ggatcacccc acccgacact
     6601 tcacaatcaa ggggtacaat acacaagggt ggaggaacac cccaccctcc agacacatta
     6661 cacagaaatc caatcaaaca gaagcaccat cagggcttct gctaccaaat ttatctcaaa
     6721 aaactacaac aaggaatcac catcagggat tccctgtgca atatacgtca aacgagggcc
     6781 acgacgggag gacgatcacg cctcccgaat atcggcatgt ctggctttcg aattcagtgc
     6841 gtggagcatc agcccacgca gccaatcaga gtcgaataca agtcgacttt cgcgaagagc
     6901 atcagccttc gcgccattct tacacaaacc acactctccc ctacaggaac agcatcagcg
     6961 ttcctgccca gtacccaact caagaaaatt tatgtcccca tgcagcatca gcgcatgggc
     7021 cccaagaata catccccaac aaaatcacat ccgagcacca acagggctcg gagtgttgtt
     7081 tcttgtccaa ctggacaaac cctccatgga ccatcaggcc atggactctc accaacaaga
     7141 caaaaactac tcttctcgaa gcagcatcag cgcttcgaaa cactcgagca tacattgtgc
     7201 ctatttcttg ggtggacgat cacgccaccc atgctctcac gaatttcaaa acacggacaa
     7261 ggacgagcac caccagggct cgtcgttcca cgtccaatac gattacttac ctttcgggat
     7321 cacgatcacg gatcccgcag ctacatcact tccactcagg acattcaagc atgcacgatc
     7381 acggcatgct ccacaagtct caaccacaga aactaccaaa tgggttcagc accagcgaac
     7441 ccactcctac ctcaaacctc ttcccacaaa actggcaagc aggatcaccg cttgcccatt
     7501 ccaacatacc aaatcaaaaa caattactgg tacagcatca gcgtaccagc ccacatctct
     7561 cactactatc aaaaaccaaa ccgttcagca acagcgaacg gtacacacgg aaaaatcaac
     7621 tggtttacaa atacgaaaga cgatcacgct ttcgtccagc gcaaactatt acgaaaaaca
     7681 tccgacggga agagcaacag ccttcccgcg gcggaaaacc tcacaaaaac acgacaaacg
     7741 gatgcacgaa cacggcatcc gccgacaacc cacaaactta caaccaggca aacggtgcag
     7801 gatcaccgca ccgtacatca aacacctcag atctcatatc gatagatcct aatcaacctc
     7861 tggattacaa atgtacaagc tcgagggtaa gcctatccct aaccctctcc tcggtctcga
     7921 ttctacgtag taatgaaccg gtccaccacc accaccacca ctaaggatcc ggggttgggg
     7981 ttgcgccttt tccaagcacc tgctacatag cggtacccct cgactgtgcc ttctagttgc
     8041 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     8101 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     8161 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     8221 catgctgggg atgcggtggg ctcttcgaat ttaaatcgga tccagctgaa tctaagtgga
     8281 tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
     8341 agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact
     8401 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
     8461 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc
     8521 tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc
     8581 atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt
     8641 ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt
     8701 ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct
     8761 acgtctttgt ttcgttttct gttctgcgcc gttacagatc taagctgtga ccggcgccta
     8821 ctctagagcc gccatgtcta gactggacaa gagcaaagtc ataaactctg ctctggaatt
     8881 actcaatgga gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt
     8941 tgagcagcct accctgtact ggcacgtgaa gaacaagcgg gccctgctcg atgccctgcc
     9001 aatcgagatg ctggacaggc atcataccca ctcctgcccc ctggaaggcg agtcatggca
     9061 agactttctg cggaacaacg ccaagtcata ccgctgtgct ctcctctcac atcgcgacgg
     9121 ggctaaagtg catctcggca cccgcccaac agagaaacag tacgaaaccc tggaaaatca
     9181 gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac gcactgtacg ctctgtccgc
     9241 cgtgggccac tttacactgg gctgcgtatt ggaggaacag gagcatcaag tagcaaaaga
     9301 ggaaagagag acacctacca ccgattctat gcccccactt ctgaaacaag caattgagct
     9361 gttcgaccgg cagggagccg aacctgcctt ccttttcggc ctggaactaa tcatatgtgg
     9421 cctggagaaa cagctaaagt gcgaaagcgg cgggccgacc gacgcccttg acgattttga
     9481 cttagacatg ctcccagccg atgcccttga cgactttgac cttgatatgc tgcctgctga
     9541 cgctcttgac gattttgacc ttgacatgct ccccggggag ggcagaggaa gtcttctaac
     9601 atgcggtgac gtggaggaga atcccggccc tatgaccgag tacaagccca cggtgcgcct
     9661 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     9721 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     9781 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     9841 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     9901 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     9961 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
    10021 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
    10081 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
    10141 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
    10201 ctggtgcatg acccgcaagc ccggtgcctg agtcgacaat caacctctgg attacaaaat
    10261 ttgtgggtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
    10321 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
    10381 aatgtatctt atcatgtctg gaattgactc aaatgatgtc aattagtcta tcagaagcta
    10441 tctggtctcc cttccggggg acaagacatc cctgtttaat atttaaacag cagtgttccc
    10501 aaactgggtt cttatatccc ttgctctggt caaccaggtt gcagggtttc ctgtcctcac
    10561 aggaacgaag tccctaaaga aacagtggca gccaggttta gccccggaat tgactggatt
    10621 ccttttttag ggcccattgg tatggctttt tccccgtatc cccccaggtg tctgcaggct
    10681 caaagagcag cgagaagcgt tcagaggaaa gcgatcccgt gccaccttcc ccgtgcccgg
    10741 gctgtccccg cacgctgccg gctcggggat gcggggggag cgccggaccg gagcggagcc
    10801 ccgggcggct cgctgctgcc ccctagcggg ggagggacgt aattacatcc ctgggggctt
    10861 tggggggggg ctgtccctga tatctataac aagaaaatat atatataata agttatcacg
    10921 taagtagaac atgaaataac aatataatta tcgtatgagt taaatcttaa aagtcacgta
    10981 aaagataatc atgcgtcatt ttgactcacg cggtcgttat agttcaaaat cagtgacact
    11041 taccgcattg acaagcacgc ctcacgggag ctccaagcgg cgactgagat gtcctaaatg
    11101 cacagcgacg gattcgcgct atttagaaag agagagcaat atttcaagaa tgcatgcgtc
    11161 aattttacgc agactatctt tctagggtta atctagctgc atcaggatca tatcgtcggg
    11221 tcttttttcc ggctcagtca tcgcccaagc tggcgctatc tgggcatcgg ggaggaagaa
    11281 gcccgtgcct tttcccgcga ggttgaagcg gcatggaaag agtttgccga ggatgactgc
    11341 tgctgcattg acgttgagcg aaaacgcacg tttaccatga tgattcggga aggtgtggcc
    11401 atgcacgcct ttaacggtga actgttcgtt caggccacct gggataccag ttcgtcgcgg
    11461 cttttccgga cacagttccg gatggtcagc ccgaagcgca tcagcaaccc gaacaatacc
    11521 ggcgacagcc ggaactgccg tgccggtgtg cagattaatg acagcggtgc ggcgctggga
    11581 tattacgtca gcgaggacgg gtatcctggc tggatgccgc agaaatggac atggataccc
    11641 cgtgagttac ccggcgggcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa
    11701 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
    11761 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
    11821 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
    11881 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
    11941 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
    12001 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
    12061 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
    12121 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
    12181 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
    12241 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
    12301 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
    12361 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    12421 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    12481 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
    12541 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    12601 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    12661 gatctcaa
//
