LOCUS       sequence-491543-        7904 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lux gene for Golden Gate Assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7904)
  AUTHORS   Larsson EM, Wang OY, Murray RM
  TITLE     A DNA Part Library for Reliable Engineering of the Emerging Model 
            Nematode Symbiotic Bacterium Xenorhabdus griffiniae HGB2511.
  JOURNAL   ACS Synth Biol. 2025 Oct 17;14(10):4122-4126. doi: 
            10.1021/acssynbio.5c00414. Epub 2025 Sep 25.
  PUBMED    40998411
REFERENCE   2  (bases 1 to 7904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5c00414"; journalName: "ACS Synth Biol"; date: 
            "2025-10-17- 17"; volume: "14"; issue: "10"; pages: "4122-4126"
COMMENT     Sequence Label: eOYWP01
FEATURES             Location/Qualifiers
     source          1..7904
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2..1444
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             1457..2380
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             2429..3511
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             3526..4509
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             4688..5800
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     misc_feature    5812..5832
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(5838..5855)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6009..6597)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6089..6108)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6768..7559,7560..7628))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7391..7410
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7629..7733)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7801..7819
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(7823..7866)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    7869..7890
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aatgactaaa aaaatttcat tcattattaa cggccaggtt gaaatctttc ccgaaggtga
       61 tgatttagtg caatccatta attttggtga taatagtgtt tacctgccaa tattgaatga
      121 ctctcatgta aaaaacatta ttgattgtaa tggaaataac gaattacggt tgcataacat
      181 tgtcaatttt ctctatacgg tagggcaaag atggaaaaat gaagaatact caagacgcag
      241 gacatacatt cgtgacttaa aaaaatatat gggatattca gaagaaatgg ctaagctaga
      301 ggccaattgg atatctatga ttttatgttc taaaggcggc ctttatgatg ttgtagaaaa
      361 tgaacttggt tctcgccata tcatggatga atggctacct caggatgaaa gttatgttcg
      421 ggcttttccg aaaggtaaat ctgtacatct gttggcaggt aatgttccat tatctgggat
      481 catgtctata ttacgcgcaa ttttaactaa gaatcagtgt attataaaaa catcgtcaac
      541 cgatcctttt accgctaatg cattagcgtt aagttttatt gatgtagacc ctaatcatcc
      601 gataacgcgc tctttatctg ttatatattg gccccaccaa ggtgatacat cactcgcaaa
      661 agaaattatg caacatgcgg atgttattgt cgcttgggga gggccagatg cgattaattg
      721 ggcggtagag catgcgccat cttatgctga tgtgattaaa tttggttcta aaaagagtct
      781 ttgcattatc gataatcctg ttgatttgac gtccgcagcg acaggtgcgg ctcatgatgt
      841 ttgtttttac gatcagcgag cttgtttttc tgcccaaaac atatattaca tgggaaatca
      901 ttatgaggaa tttaagttag cgttgataga aaaacttaat ctatatgcgc atatattacc
      961 gaatgccaaa aaagattttg atgaaaaggc ggcctattct ttagttcaaa aagaaagctt
     1021 gtttgctgga ttaaaagtag aggtggatat tcatcaacgt tggatgatta ttgagtcaaa
     1081 tgcaggtgtg gaatttaatc aaccacttgg cagatgtgtg taccttcatc acgtcgataa
     1141 tattgagcaa atattgcctt atgttcaaaa aaataagacg caaaccatat ctatttttcc
     1201 ttgggagtca tcatttaaat atcgagatgc gttagcatta aaaggtgcgg aaaggattgt
     1261 agaagcagga atgaataaca tatttcgagt tggtggatct catgacggga tgaggccgtt
     1321 gcaacgatta gtgacatata tttctcatga aaggccatct aactatacgg ctaaggatgt
     1381 tgcggttgaa atagaacaga ctcgattcct ggaagaagat aagttccttg tatttgtccc
     1441 ataataggta aaaagtatgg aaaatgaatc aaaatataaa accatcgacc acgttatttg
     1501 tgttgaagga aataaaaaaa ttcatgtttg ggaaacgctg ccagaagaaa acagcccaaa
     1561 gagaaagaat gccattatta ttgcgtctgg ttttgcccgc aggatggatc attttgctgg
     1621 tctggcggaa tatttatcgc ggaatggatt tcatgtgatc cgctatgatt cgcttcacca
     1681 cgttggattg agttcaggga caattgatga atttacaatg tctataggaa agcagagctt
     1741 gttagcagtg gttgattggt taactacacg aaaaataaat aacttcggta tgttggcttc
     1801 aagcttatct gcgcggatag cttatgcaag cctatctgaa atcaatgctt cgtttttaat
     1861 caccgcagtc ggtgttgtta acttaagata ttctcttgaa agagctttag ggtttgatta
     1921 tctcagtcta cccattaatg aattgccgaa taatctagat tttgaaggcc ataaattggg
     1981 tgctgaagtc tttgcgagag attgtcttga ttttggttgg gaagatttag cttctacaat
     2041 taataacatg atgtatcttg atataccgtt tattgctttt actgcaaata acgataattg
     2101 ggtcaagcaa gatgaagtta tcacattgtt atcaaatatt cgtagtaatc gatgcaagat
     2161 atattctttg ttaggaagtt cgcatgactt gagtgaaaat ttagtggtcc tgcgcaattt
     2221 ttatcaatcg gttacgaaag ccgctatcgc gatggataat gatcatctgg atattgatgt
     2281 tgatattact gaaccgtcat ttgaacattt aactattgcg acagtcaatg aacgccgaat
     2341 gagaattgag attgaaaatc aagcaatttc tctgtcttaa aatctattga gatattctat
     2401 cactcaaata gcaatataag gactctctat gaaatttgga aactttttgc ttacatacca
     2461 acctccccaa ttttctcaaa cagaggtaat gaaacgtttg gttaaattag gtcgcatctc
     2521 tgaggagtgt ggttttgata ccgtatggtt actggagcat catttcacgg agtttggttt
     2581 gcttggtaac ccttatgtcg ctgctgcata tttacttggc gcgactaaaa aattgaatgt
     2641 aggaactgcc gctattgttc ttcccacagc ccatccagta cgccaacttg aagatgtgaa
     2701 tttattggat caaatgtcaa aaggacgatt tcggtttggt atttgccgag ggctttacaa
     2761 caaggacttt cgcgtattcg gcacagatat gaataacagt cgcgccttag cggaatgctg
     2821 gtacgggctg ataaagaatg gcatgacaga gggatatatg gaagctgata atgaacatat
     2881 caagttccat aaggtaaaag taaaccccgc ggcgtatagc agaggtggcg caccggttta
     2941 tgtggtggct gaatcagctt cgacgactga gtgggctgct caatttggcc taccgatgat
     3001 attaagttgg attataaata ctaacgaaaa gaaagcacaa cttgagcttt ataatgaagt
     3061 ggctcaagaa tatgggcacg atattcataa tatcgaccat tgcttatcat atataacatc
     3121 tgtagatcat gactcaatta aagcgaaaga gatttgccgg aaatttctgg ggcattggta
     3181 tgattcttat gtgaatgcta cgactatttt tgatgattca gaccaaacaa gaggttatga
     3241 tttcaataaa gggcagtggc gtgactttgt attaaaagga cataaagata ctaatcgccg
     3301 tattgattac agttacgaaa tcaatcccgt gggaacgccg caggaatgta ttgacataat
     3361 tcaaaaagac attgatgcta caggaatatc aaatatttgt tgtggatttg aagctaatgg
     3421 aacagtagac gaaattattg cttccatgaa gctcttccag tctgatgtca tgccatttct
     3481 taaagaaaaa caacgttcgc tattatatta gctaaggaga aagaaatgaa atttggattg
     3541 ttcttcctta acttcatcaa ttcaacaact gttcaagaac aaagtatagt tcgcatgcag
     3601 gaaataacgg agtatgttga taagttgaat tttgaacaga ttttagtgta tgaaaatcat
     3661 ttttcagata atggtgttgt cggcgctcct ctgactgttt ctggttttct gctcggttta
     3721 acagagaaaa ttaaaattgg ttcattaaat cacatcatta caactcatca tcctgtccgc
     3781 atagcggagg aagcttgctt attggatcag ttaagtgaag ggagatttat tttagggttt
     3841 agtgattgcg aaaaaaaaga tgaaatgcat ttttttaatc gcccggttga atatcaacag
     3901 caactatttg aagagtgtta tgaaatcatt aacgatgctt taacaacagg ctattgtaat
     3961 ccagataacg atttttatag cttccctaaa atatctgtaa atccccatgc ttatacgcca
     4021 ggcggacctc ggaaatatgt aacagcaacc agtcatcata ttgttgagtg ggcggccaaa
     4081 aaaggtattc ctctcatctt taagtgggat gattctaatg atgttagata tgaatatgct
     4141 gaaagatata aagccgttgc ggataaatat gacgttgacc tatcagagat agaccatcag
     4201 ttaatgatat tagttaacta taacgaagat agtaataaag ctaaacaaga gacgcgtgca
     4261 tttattagtg attatgttct tgaaatgcac cctaatgaaa atttcgaaaa taaacttgaa
     4321 gaaataattg cagaaaacgc tgtcggaaat tatacggagt gtataactgc ggctaagttg
     4381 gcaattgaaa agtgtggtgc gaaaagtgta ttgctgtcct ttgaaccaat gaatgatttg
     4441 atgagccaaa aaaatgtaat caatattgtt gatgataata ttaagaagta ccacatggaa
     4501 tatacctaat agatttcgag ttgcagcgag gcggcaagtg aacgaatccc caggagcata
     4561 gataactatg tgactggggt gagtgaaagc agccaacaaa gcagcagctt gaaagatgaa
     4621 gggtataaaa gagtatgaca gcagtgctgc catactttct aatattatct tgaggagtaa
     4681 aacaggtatg acttcatatg ttgataaaca agaaattaca gcaagctcag aaattgatga
     4741 tttgattttt tcgagcgatc cattagtgtg gtcttacgac gagcaggaaa aaatcagaaa
     4801 gaaacttgtg cttgatgcat ttcgtaatca ttataaacat tgtcgagaat atcgtcacta
     4861 ctgtcaggca cacaaagtag atgacaatat tacggaaatt gatgacatac ctgtattccc
     4921 aacatcggtt tttaagttta ctcgcttatt aacttctcag gaaaacgaga ttgaaagttg
     4981 gtttaccagt agcggcacga atggtttaaa aagtcaggtg gcgcgtgaca gattaagtat
     5041 tgagagactc ttaggctctg tgagttatgg catgaaatat gttggtagtt ggtttgatca
     5101 tcaaatagaa ttagtcaatt tgggaccaga tagatttaat gctcataata tttggtttaa
     5161 atatgttatg agtttggtgg aattgttata tcctacgaca tttaccgtaa cagaagaacg
     5221 aatagatttt gttaaaacat tgaatagtct tgaacgaata aaaaatcaag ggaaagatct
     5281 ttgtcttatt ggttcgccat actttattta tttactctgc cattatatga aagataaaaa
     5341 aatctcattt tctggagata aaagccttta tatcataacc ggaggcggct ggaaaagtta
     5401 cgaaaaagaa tctctgaaac gtgatgattt caatcatctt ttatttgata ctttcaatct
     5461 cagtgatatt agtcagatcc gagatatatt taatcaagtt gaactcaaca cttgtttctt
     5521 tgaggatgaa atgcagcgta aacatgttcc gccgtgggta tatgcgcgag cgcttgatcc
     5581 tgaaacgttg aaacctgtac ctgatggaac gccggggttg atgagttata tggatgcgtc
     5641 agcaaccagt tatccagcat ttattgttac cgatgatgtc gggataatta gcagagaata
     5701 tggtaagtat cccggcgtgc tcgttgaaat tttacgtcgc gtcaatacga ggacgcagaa
     5761 agggtgtgct ttaagcttaa ccgaagcgtt tgatagttga aggtagagac ctactagtag
     5821 cggccgctgc aggcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     5881 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     5941 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     6001 ctggcgtttt tccacaggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     6061 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     6121 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     6181 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     6241 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     6301 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     6361 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     6421 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     6481 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     6541 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     6601 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     6661 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     6721 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     6781 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     6841 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     6901 ataccgcgcg acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     6961 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     7021 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     7081 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     7141 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     7201 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     7261 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     7321 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     7381 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     7441 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     7501 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     7561 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     7621 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     7681 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     7741 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     7801 aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat
     7861 gatttctgga attcgcggcc gcttctagag actagtgggt ctca
//
