LOCUS       sequence-49157-e         707 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 707)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ZFYVE16
FEATURES             Location/Qualifiers
     source          1..707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             31..201
                     /codon_start=1
                     /product="Z domain of staphylococcal protein A, engineered 
                     to contain multiple positive charges (Hedhammar and Hober, 
                     2007)"
                     /label=Z-basic tag
                     /note="useful for purifying tagged proteins by 
                     cation-exchange chromatography"
                     /translation="VDNKFNKERRRARREIRHLPNLNREQRRAFIRSLRDDPSQSANLL
                     AEAKKLNDAQPK"
     CDS             208..228
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             687..704
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtggataaca agttcaacaa ggagcgtcga
       61 agagctcgcc gtgaaattcg ccatctgccg aacctgaacc gcgaacagcg tcgcgcattt
      121 attcgcagcc tgcgcgatga tccgagccag agcgcgaacc tgctggcgga agcgaagaag
      181 ctgaacgatg cgcagccgaa gggtaccgag aacctgtact tccaatccat ggaaggcttg
      241 gttttgggcc agaaacagcc tacttgggtt cctgattcag aagctccaaa ctgtatgaac
      301 tgccaagtca aatttacttt taccaaacgg cgacaccatt gccgagcatg tgggaaagta
      361 ttttgtggtg tctgttgtaa taggaagtgt aaactgcaat atctagaaaa ggaagcaaga
      421 gtatgtgtag tctgctatga aactattagt aaagctcagg catttgaaag gatgatgagt
      481 ccaactggtt ctaatcttaa gtctaatcat tctgatgaat gtactactgt ccagcctcct
      541 caggagaacc aaacatccag tataccttca ccagcaactt tgccagtctc agcacttaaa
      601 caaccaggtg ttgaaggact atgacagtaa aggtggatac ggatccgaat tcgagctccg
      661 tcgacaagct tgcggccgca ctcgagcacc accaccacca ccactga
//
