LOCUS       sequence-49158-e         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: HGS
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tggggcgagg cagcggcacc ttcgagcgtc tcctagacaa ggcgaccagc
      121 cagctcctgt tggagacaga ttgggagtcc attttgcaga tctgcgacct gatccgccaa
      181 ggggacacac aagcaaaata tgctgtgaat tccatcaaga agaaagtcaa cgacaagaac
      241 ccacacgtcg ccttgtatgc cctggaggtc atggaatctg tggtaaagaa ctgtggccag
      301 acagttcatg atgaggtggc caacaagcag accatggagg agctgaagga cctgctgaag
      361 agacaagtgg aggtaaacgt ccgtaacaag atcctgtacc tgatccaggc ctgggcgcat
      421 gccttccgga acgagcccaa gtacaaggtg gtccaggaca cctaccagat catgaaggtg
      481 gaggggcacg tctttccaga attcaaagag agcgatgcca tgtttgctgc cgagagagcc
      541 ccagactggg tggacgctga ggaatgccac cgctgcaggg tgcagttcgg ggtgatgacc
      601 cgtaagcacc actgccgggc gtgtgggcag atattctgtg gaaagtgttc ttccaagtac
      661 tccaccatcc ccaagtttgg catcgagaag gaggtgcgcg tgtgtgagcc ctgctacgag
      721 cagctgaaca ggaaagcgga gggatgacag taaaggtgga tacggatccg aa
//
