LOCUS       sequence-491583-       15318 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral constitutive expression construct for 
            CIC(ex18)-NUTM1(ex3) (pLEX_305 backbone).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15318)
  TITLE     CIC-family fusion oncoprotein expression constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15318)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLEX_305_CIC(ex18)-NUTM1(ex3)
FEATURES             Location/Qualifiers
     source          1..15318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2823..3033
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        2845..3041
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2892..3027
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3073..3675
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(3073..3092)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3569..3589
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3718..3835
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3893..4393
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4312..4330
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    4414..4438
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      6445..6454
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(7482..7499)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     protein_bind    complement(12152..12176)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    12228..12816
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(12281..12301)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(12699..12710)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             12888..13121
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    13199..13320
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13236..13255)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13290..13309
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      13360..13495
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(13515..13534)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(13516..13534)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(13544..13561)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(13544..13560)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13553..13575)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      13702..14157
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13789..13808)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13999..14020
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        14183..14287
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(14288..14356,14357..15148)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(14506..14525)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccatgc attgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     2821 cagcaggcag aagtatgcaa agcatgcgtc tcaattagtc agcaaccata gtcccgcccc
     2881 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     2941 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     3001 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctttct agaggtacca
     3061 ccggagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     3121 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     3181 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     3241 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgccgt ggcggtctgg
     3301 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     3361 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     3421 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     3481 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     3541 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     3601 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     3661 cccggtgcca gttaacgaat tctagatctt gagacaaatg gcagtattca tccacaattt
     3721 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     3781 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     3841 ttattacagg gacagcagag atccactttg gcgccggctc gaggcctgca ggttgcgcct
     3901 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa
     3961 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg
     4021 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg
     4081 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac
     4141 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc
     4201 tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt
     4261 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc
     4321 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga
     4381 cctctctccc caggggctag catcgattga tcaacaagtt tgtacaaaaa agcaggctta
     4441 atgtattcgg cccacaggcc cctgatgccc gcgtccagcg cggcctcccg tggcctcggc
     4501 atgttcgtgt ggacgaatgt ggaacctcgc tctgtggctg tgttcccttg gcactcctta
     4561 gtccccttcc tggcacccag ccagcctgac ccctccgtgc agccgagcga ggcccagcaa
     4621 cctgccagcc acccagtggc ctccaaccag agcaaagaac ctgctgagtc ggcagctgtt
     4681 gctcatgaac ggccaccagg tgggacaggg agtgctgacc ctgagcggcc ccctggagcc
     4741 acatgccctg agagcccagg acccggaccc ccacaccctt tgggggtggt ggaatctggt
     4801 aagggtccgc ctcccaccac ggaggaggag gcctccggcc ccccaggaga gccccggctg
     4861 gacagtgaga cagagagtga ccatgatgat gccttcctct ccatcatgtc tcctgagatc
     4921 cagttgcctc taccgcccgg aaaacgtcgg acccagtccc tcagtgccct acccaaggaa
     4981 cgggactcat cttctgagaa ggatggacgc agccccaaca agcgggagaa ggaccacatc
     5041 cggcggccca tgaatgcctt catgatcttc agcaagcggc accgggccct ggtccaccag
     5101 cgtcatccca accaggacaa ccggaccgtc agcaagatcc tgggcgagtg gtggtatgcc
     5161 ctggggccca aggagaagca gaagtaccac gacctggcct tccaggtgaa ggaggcccac
     5221 ttcaaggccc acccagattg gaagtggtgt aacaaggacc gaaagaagtc cagctcagag
     5281 gccaagccca cgagcctggg gctggcagga gggcacaagg agacgcggga gcggagcatg
     5341 tcggagacgg gcactgctgc tgcccctggg gtgtcctctg agctcctgtc cgttgcagcc
     5401 cagacactcc tgagctcaga caccaaggct ccggggagca gctcctgtgg ggcagaacgg
     5461 ctacacacag ttgggggacc tggctcagcc cggccccgag ctttctccca cagcggggta
     5521 cacagcctgg acggcggaga agtagacagt caggcgctac aggaactgac gcagatggtg
     5581 tctggccctg catcgtactc tggcccaaag ccttctaccc agtatggagc tccaggaccc
     5641 tttgcagccc ctggtgaggg aggtgccttg gcggccactg ggcggccccc gctgctgccc
     5701 acccgagctt ctcgttctca gcgtgcggcc agtgaggaca tgacgagtga tgaggagcgc
     5761 atggtcatct gtgaggagga aggggatgat gatgtcattg ctgacgatgg cttcggcacc
     5821 actgacattg atctcaagtg caaggagcgg gtgaccgaca gcgagagtgg ggacagctct
     5881 ggggaggacc cagagggcaa caagggcttt ggtcggaagg tgttttcacc tgtgatccgt
     5941 tcctccttta cccactgccg ccccccactg gaccctgagc ccccagggcc cccggatcct
     6001 cctgtagcct ttggcaaagg ctatggttcc gccccatcct cctctgcgtc ctcgcctgct
     6061 tcctcctcag cctcggcagc cacctccttc tcactgggct caggaacctt caaggcccag
     6121 gagtctggtc agggcagcac agcgggcccc ctacggcccc caccccctgg ggctgggggt
     6181 ccagcgacac cttccaaggc aacccggttc ctcccaatgg atcctgccac cttccggcgc
     6241 aagagacccg aaagtgtggg tggcctggag ccaccaggcc cctcagtcat cgcggcccct
     6301 cccagcggag gaggaaacat cctgcagaca ctggtgctgc ccccaaacaa ggaggagcaa
     6361 gagggcggcg gagccagagt gccctccgcc cccgccccat cactggccta tggggcccca
     6421 gcagctcccc tgtcccgtcc tgccgccacc atggtcacca atgtggtgcg gcctgtcagc
     6481 agcactcctg tgcccatcgc ctctaagccc ttccccacct ctggccgggc tgaggcgtct
     6541 ccaaatgaca cagcaggtgc caggactgaa atgggcactg ggtctcgggt gcctgggggc
     6601 tccccgctgg gtgtcagctt agtgtattcg gacaagaagt cggcagcagc cacctcacca
     6661 gccccacact tggtggctgg acccctgctg ggcactgtgg ggaaggcgcc tgccactgtc
     6721 actaacctac tggtgggcac cccggggtat ggggcccctg cgccccctgc tgtccagttc
     6781 attgcccagg gggcccctgg tggtgggacc actgcgggct caggagcagg tgctgggagt
     6841 ggccccaatg ggccagtacc cctgggcatc ctgcaaccag gtgccctggg caaggctggg
     6901 ggaatcaccc aggtacagta catcctgccc acgctgcccc agcagcttca ggtggcacct
     6961 gccccagcac cagcccctgg gaccaaggca gcggctccca gcggccctgc acccaccacc
     7021 agcatccgtt tcaccctccc accgggcact tccaccaacg gcaaagtcct ggctgccact
     7081 gcacccactc ctggcatccc catcctgcag tctgtaccct ccgccccacc ccccaaagcc
     7141 cagtcagttt ctcccgtgca ggccccgccc ccgggtggct cagcccagct gctgcctggg
     7201 aaggtcctag tgcctctggc cgcccctagc atgtcagtgc ggggtggagg ggccggccag
     7261 ccactgccac tggtgagccc gcccttctca gtacctgtgc agaatggtgc ccagcccccc
     7321 agcaagatca tccagctgac cccggtgcct gtgagcacac ccagcggcct ggtgccgccc
     7381 ctgagcccag ccacactccc tggacccacc tctcagcctc agaaggtcct gttgccctcc
     7441 tccaccagaa tcacctatgt gcagtcagcg ggcgggcacg cgctgcccct gggtaccagc
     7501 cctgcgtcca gccaggctgg aacagtcacc tcgtacgggc ccacgagctc tgtagctcta
     7561 ggcttcacct cgctggggcc cagcggcccc gccttcgtgc agcccctgct ctcagcaggc
     7621 caagccccac tgctggctcc cggtcaggtg ggcgtgtcac ctgtgcccag tccccagctg
     7681 ccgcctgcct gtgcagcccc cggaggtcct gtcataacag cattttactc tggcagccct
     7741 gcacccacct cctcagcacc cctggcccag ccatcccagg cccccccaag cctggtctac
     7801 actgtggcca ccagcacaac cccacctgca gccaccattc tgcccaaggg cccgccagcc
     7861 cctgccactg ccaccccagc cccgactagc cctttcccca gcgccacagc aggttccatg
     7921 acctacagct tagtggcccc caaggcccag cggcccagcc cgaaggcccc ccagaaagtg
     7981 aaggcagcca tcgccagcat tcccgtgggg tcctttgagg caggtgcctc tgggcggcct
     8041 ggccctgcac cccggcagcc tctggagcct ggcccagtcc gagagccaac tgccccagag
     8101 tctgagcttg aggggcagcc cacaccacca gcccctccac ccctgccaga gacctggact
     8161 cccacggccc ggagcagccc cccactgccc ccacctgctg aggagcggac cagcgccaag
     8221 ggccctgaga ccatggccag caaattcccc agctcatctt cagactggcg cgtccctggg
     8281 cagggcctgg agaatcgtgg ggagcctccc actcctccca gcccggcccc agctccagct
     8341 gtagcccctg gtggcagcag cgagagcagc agtgggcggg cagccgggga caccccggag
     8401 cgcaaggagg cggctggtac tggcaagaag gtgaaggtgc ggcccccgcc cctgaagaag
     8461 acctttgact ctgtggacaa cagggtcctg tcagaagtgg acttcgaaga gcgctttgct
     8521 gagttgcctg agtttcggcc tgaggaggtg ctgccctccc ccaccctgca gtctctggcc
     8581 acctcacccc gggccatcct gggctcttac cgcaagaaga ggaagaactc cacggacctg
     8641 gattcagcac ccgaggaccc cacctcgccc aagcgcaaga tgagaagacg ctccagctgc
     8701 agctcggagc ccaacacccc caagagtgcc aagtgcgagg gggacatctt cacctttgac
     8761 cgtacagcat ctgcattgcc gggaccggat atgagcatga aacctagtgc cgccctgtct
     8821 ccatcccctg cacttccctt tctcccacca acttctgacc caccagacca cccacccagg
     8881 gagccacctc cacagcccat catgccttca gtattctctc cagacaaccc tctgatgctc
     8941 tctgctttcc ccagctcact gttggtgaca ggggacgggg gcccttgcct cagtggggct
     9001 ggggctggca aggtcattgt caaagtcaag acagaagggg ggtcagctga gccctctcaa
     9061 actcagaact ttatccttac tcagactgcc ctcaattcga ctgccccggg cactccctgt
     9121 ggaggccttg agggtcctgc acctccattt gtgacagcat ctaatgtgaa gaccattctg
     9181 ccctctaagg ctgttggtgt cagccaggag ggtcctccag gccttccgcc tcagcctcca
     9241 ccaccagttg ctcaactggt ccccattgtg cccctggaaa aagcttggcc agggccacat
     9301 gggacaaccg gggaaggagg tcctgtggcc actctatcca agccttccct aggtgaccgc
     9361 tccaaaattt ccaaggacgt ttatgagaac ttccgtcagt ggcagcgtta caaagccttg
     9421 gcccggaggc acctatccca gagtcctgac acagaagctc tttcctgttt tcttatccca
     9481 gtgcttcgtt ccctggcccg gctgaagccc actatgaccc tggaggaggg actgccattg
     9541 gctgtgcagg agtgggagca caccagcaac tttgaccgga tgatctttta tgagatggca
     9601 gaaaggttca tggagtttga ggctgaggag atgcagattc agaacacaca gctgatgaat
     9661 gggtctcagg gcctgtctcc tgcaacccct ttgaaacttg atcctctagg gcccctggcc
     9721 tctgaggttt gccagcagcc agtgtacatt ccgaagaagg cagcttctaa aacaagagct
     9781 cctcgccggc gtcagcgtaa agcccagaga cctcctgctc ctgaggcacc caaggagatc
     9841 ccaccagaag ctgtgaagga gtatgttgac atcatggaat ggctggtggg gactcacttg
     9901 gccactgggg agtcagatgg aaaacaagag gaagaagggc agcagcagga ggaggaaggg
     9961 atgtatccag atccaggtct cctgagctac atcaatgagc tgtgttctca gaaggtcttt
    10021 gtctccaagg tggaggctgt cattcaccct caatttctgg cagatctgct gtccccagaa
    10081 aaacagagag atcccttggc cttaattgag gagctagagc aagaagaagg actcactctt
    10141 gcccagctgg tccagaagcg actcatggcc ttggaagagg aggaagatgc agaggcgcct
    10201 ccaagtttca gtggcgctca gttggactca agtccttctg gttctgttga ggatgaagat
    10261 ggggatgggc ggcttcggcc ctcacctggg cttcaggggg ctgggggcgc cgcttgcctt
    10321 ggaaaggttt cttcttcagg aaaacgggca agagaagtgc atggtgggca ggagcaagcc
    10381 ctagatagcc ccagagggat gcacagggat gggaacactc tgccatcccc cagcagctgg
    10441 gacctgcagc cagaacttgc agctccacag ggaactccgg gacccttggg tgtggagagg
    10501 agagggtctg ggaaggttat aaaccaggta tctctacatc aggatggcca tctaggaggc
    10561 gctgggcctc ctgggcactg cctggtggct gataggactt cagaggctct gcccctttgt
    10621 tggcagggag gcttccagcc tgagagcact cccagtttgg atgctggact tgcagagctg
    10681 gctcctctgc aaggacaagg gttagaaaag caagtcctgg gattgcagaa aggacaacaa
    10741 acagggggtc gtggagtgct tcctcaaggg aaggagcctt tagcagtgcc ctgggaaggc
    10801 tcttcaggag ccatgtgggg agatgacaga ggtaccccca tggctcagag ttatgatcag
    10861 aatccttccc ctagagcagc tggggagagg gacgatgtct gtctcagccc aggagtttgg
    10921 ctgagcagtg agatggatgc tgtaggcttg gagctgcctg tacaaataga ggaggtcata
    10981 gagagcttcc aagttgagaa gtgtgtaact gagtatcagg aaggctgcca gggactgggc
    11041 tccaggggca acatttccct gggtcctgga gaaaccctag tacctgggga tacggagagc
    11101 agtgtgattc cctgtggagg cacagttgcg gcagctgccc tagaaaagag aaactattgc
    11161 agcttgccag gacctttgag ggccaacagc ccacccttga ggtccaaaga aaatcaagaa
    11221 cagagctgtg aaaccgtagg gcatcccagt gatctgtggg cagaaggctg cttcccattg
    11281 ctagaaagtg gtgattccac actggggtct tccaaagaaa cccttccacc cacatgccaa
    11341 ggcaatctcc ttatcatggg gactgaggat gcctcctcct tgcctgaagc cagtcaagag
    11401 gcagggagca gaggcaattc cttttctcct ctgttggaaa ccatagaacc tgtcaacata
    11461 ctagatgtta aagatgactg tggcctccaa ctaagggtca gcgaggacac ctgcccactg
    11521 aatgttcatt cttatgaccc ccaaggagaa ggcagggtgg atcctgatct gtccaagcct
    11581 aaaaaccttg ctcctttaca agagagtcag gagtcttaca caactgggac tcccaaagca
    11641 acatcttctc accagggcct tggaagcact ttgcctagaa ggggaaccag gaatgccata
    11701 gttccgagag aaacttctgt tagtaaaaca cacaggtcag cagacagggc caaaggaaag
    11761 gagaaaaaga aaaaggaagc agaggaagag gatgaggaac tctccaactt tgcttacctc
    11821 ttggcctcta aacttagcct ctcaccaagg gagcatcccc tcagtcctca ccatgcctca
    11881 ggaggtcagg gcagccagag agcatcccac ctgctccctg ctggagcaaa aggccccagc
    11941 aaacttccat atcctgttgc caagtctggg aagcgagctc tagctggagg tccagcccct
    12001 actgaaaaga caccccactc aggagctcaa cttggggtcc ccagggagaa acccctagct
    12061 ctgggagtag ttcgaccctc acagcctcgt aaaaggcggt gtgacagttt tgtcacgggc
    12121 agaaggaaga aacgacgtcg tagccagtag aacccagctt tcttgtacaa agtggttacc
    12181 ggttagtaat gaactagtta agtcgacaat caacgcgtta agtcgacaat caacctctgg
    12241 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
    12301 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
    12361 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
    12421 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
    12481 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
    12541 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
    12601 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
    12661 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
    12721 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
    12781 agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac caatgactta
    12841 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
    12901 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
    12961 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
    13021 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
    13081 atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata gtagttcatg
    13141 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
    13201 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
    13261 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
    13321 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
    13381 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
    13441 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg
    13501 acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
    13561 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
    13621 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
    13681 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
    13741 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
    13801 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
    13861 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
    13921 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
    13981 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
    14041 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
    14101 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
    14161 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
    14221 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
    14281 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
    14341 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
    14401 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
    14461 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
    14521 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
    14581 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
    14641 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
    14701 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
    14761 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
    14821 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
    14881 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
    14941 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
    15001 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
    15061 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
    15121 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
    15181 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
    15241 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
    15301 aaagatcaaa ggatcttc
//
