LOCUS       sequence-491586-       12900 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral constitutive expression construct for HA-tagged 
            CIC::LEUTX (pLEX_305 backbone).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12900)
  TITLE     CIC-family fusion oncoprotein expression constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLEX_305_HA-CIC-LEUTX
FEATURES             Location/Qualifiers
     source          1..12900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2823..3033
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        2845..3041
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2892..3027
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3073..3675
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(3073..3092)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3569..3589
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3718..3835
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3893..4393
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4312..4330
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    4414..4438
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4450..4476
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4456..4482
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             4483..4509
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4489..4515
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             4516..4542
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4522..4548
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     regulatory      6634..6643
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(7671..7688)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     protein_bind    complement(9734..9758)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    9810..10398
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9863..9883)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10281..10292)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10470..10703
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10781..10902
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10818..10837)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10872..10891
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10942..11077
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11097..11116)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11098..11116)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11126..11143)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11126..11142)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11135..11157)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11284..11739
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11371..11390)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11581..11602
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11765..11869
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11870..11938,11939..12730)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12088..12107)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccatgc attgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     2821 cagcaggcag aagtatgcaa agcatgcgtc tcaattagtc agcaaccata gtcccgcccc
     2881 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     2941 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     3001 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctttct agaggtacca
     3061 ccggagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     3121 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     3181 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     3241 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgccgt ggcggtctgg
     3301 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     3361 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     3421 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     3481 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     3541 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     3601 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     3661 cccggtgcca gttaacgaat tctagatctt gagacaaatg gcagtattca tccacaattt
     3721 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     3781 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     3841 ttattacagg gacagcagag atccactttg gcgccggctc gaggcctgca ggttgcgcct
     3901 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa
     3961 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg
     4021 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg
     4081 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac
     4141 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc
     4201 tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt
     4261 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc
     4321 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga
     4381 cctctctccc caggggctag catcgattga tcaacaagtt tgtacaaaaa agcaggctta
     4441 atggcaggtt acccatacga cgttcctgac tatgcgtcac tctaccccta tgacgtaccg
     4501 gattatgcat ccctatatcc gtatgatgtt ccagattacg cttctctacg ttcctctaga
     4561 ggcgtccacc atatgttatc acttcgaatt cgatatcgag ctcccaggta ccgatctcga
     4621 gcggccgcca tgtattcggc ccacaggccc ctgatgcccg cgtccagcgc ggcctcccgt
     4681 ggcctcggca tgttcgtgtg gacgaatgtg gaacctcgct ctgtggctgt gttcccttgg
     4741 cactccttag tccccttcct ggcacccagc cagcctgacc cctccgtgca gccgagcgag
     4801 gcccagcaac ctgccagcca cccagtggcc tccaaccaga gcaaagaacc tgctgagtcg
     4861 gcagctgttg ctcatgaacg gccaccaggt gggacaggga gtgctgaccc tgagcggccc
     4921 cctggagcca catgccctga gagcccagga cccggacccc cacacccttt gggggtggtg
     4981 gaatctggta agggtccgcc tcccaccacg gaggaggagg cctccggccc cccaggagag
     5041 ccccggctgg acagtgagac agagagtgac catgatgatg ccttcctctc catcatgtct
     5101 cctgagatcc agttgcctct accgcccgga aaacgtcgga cccagtccct cagtgcccta
     5161 cccaaggaac gggactcatc ttctgagaag gatggacgca gccccaacaa gcgggagaag
     5221 gaccacatcc ggcggcccat gaatgccttc atgatcttca gcaagcggca ccgggccctg
     5281 gtccaccagc gtcatcccaa ccaggacaac cggaccgtca gcaagatcct gggcgagtgg
     5341 tggtatgccc tggggcccaa ggagaagcag aagtaccacg acctggcctt ccaggtgaag
     5401 gaggcccact tcaaggccca cccagattgg aagtggtgca acaaggaccg aaagaagtcc
     5461 agctcagagg ccaagcccac gagcctgggg ctggcaggag ggcacaagga gacgcgggag
     5521 cggagcatgt cggagacggg cactgctgct gcccctgggg tgtcctctga gctcctgtcc
     5581 gttgcagccc agacactcct gagctcagac accaaggctc cggggagcag ctcctgtggg
     5641 gcagaacggc tacacacagt tgggggacct ggctcagccc ggccccgagc tttctcccac
     5701 agcggggtac acagcctgga cggcggagaa gtagacagtc aggcgctaca ggaactgacg
     5761 cagatggtgt ctggccctgc atcgtactct ggcccaaagc cttctaccca gtatggagct
     5821 ccaggaccct ttgcagcccc tggtgaggga ggtgccttgg cggccactgg gcggcccccg
     5881 ctgctgccca cccgagcttc tcgttctcag cgtgcggcca gtgaggacat gacgagtgat
     5941 gaggagcgca tggtcatctg tgaggaggaa ggggatgatg atgtcattgc tgacgatggc
     6001 ttcggcacca ctgacattga tctcaagtgc aaggagcggg tgaccgacag cgagagtggg
     6061 gacagctctg gggaggaccc agagggcaac aagggctttg gtcggaaggt gttttcacct
     6121 gtgatccgtt cctcctttac ccactgccgc cccccactgg accctgagcc cccagggccc
     6181 ccggatcctc ctgtagcctt tggcaaaggc tatggttccg ccccatcctc ctctgcgtcc
     6241 tcgcctgctt cctcctcagc ctcggcagcc acctccttct cactgggctc aggaaccttc
     6301 aaggcccagg agtctggtca gggcagcaca gcgggccccc tacggccccc accccctggg
     6361 gctgggggtc cagcgacacc ttccaaggca acccggttcc tcccaatgga tcctgccacc
     6421 ttccggcgca agagacccga aagtgtgggt ggcctggagc caccaggccc ctcagtcatc
     6481 gcggcccctc ccagcggagg aggaaacatc ctgcagacac tggtgctgcc cccaaacaag
     6541 gaggagcaag agggcggcgg agccagagtg ccctccgccc ccgccccatc actggcctat
     6601 ggggccccag cagctcccct gtcccgtcct gccgccacca tggtcaccaa tgtggtgcgg
     6661 cctgtcagca gcactcctgt gcccatcgcc tctaagccct tccccacctc tggccgggct
     6721 gaggcgtctc caaatgacac agcaggtgcc aggactgaaa tgggcactgg gtctcgggtg
     6781 cctgggggct ccccgctggg tgtcagctta gtgtattcgg acaagaagtc ggcagcagcc
     6841 acctcaccag ccccacactt ggtggctgga cccctgctgg gcactgtggg gaaggcgcct
     6901 gccactgtca ctaacctact ggtgggcacc ccggggtatg gggcccctgc gccccctgct
     6961 gtccagttca ttgcccaggg ggcccctggt ggtgggacca ctgcgggctc aggagcaggt
     7021 gctgggagtg gccccaatgg gccagtaccc ctgggcatcc tgcaaccagg tgccctgggc
     7081 aaggctgggg gaatcaccca ggtacagtac atcctgccca cgctgcccca gcagcttcag
     7141 gtggcacctg ccccagcacc agcccctggg accaaggcag cggctcccag cggccctgca
     7201 cccaccacca gcatccgttt caccctccca ccgggcactt ccaccaacgg caaagtcctg
     7261 gctgccactg cacccactcc tggcatcccc atcctgcagt ctgtaccctc cgccccaccc
     7321 cccaaagccc agtcagtttc tcccgtgcag gccccgcccc cgggtggctc agcccagctg
     7381 ctgcctggga aggtcctagt gcctctggcc gcccctagca tgtcagtgcg gggtggaggg
     7441 gccggccagc cactgccact ggtgagcccg cccttctcag tacctgtgca gaatggtgcc
     7501 cagcccccca gcaagatcat ccagctgacc ccggtgcctg tgagcacacc cagcggcctg
     7561 gtgccgcccc tgagcccagc cacactccct ggacccacct ctcagcctca gaaggtcctg
     7621 ttgccctcct ccaccagaat cacctatgtg cagtcagcgg gcgggcacgc gctgcccctg
     7681 ggtaccagcc ctgcgtccag ccaggctgga acagtcacct cgtacgggcc cacgagctct
     7741 gtagctctag gcttcacctc gctggggccc agcggccccg ccttcgtgca gcccctgctc
     7801 tcagcaggcc aagccccact gctggctccc ggtcaggtgg gcgtgtcacc tgtgcccagt
     7861 ccccagctgc cgcctgcctg tgcagccccc ggaggtcctg tcataacagc attttactct
     7921 ggcagccctg cacccacctc ctcagcaccc ctggcccagc catcccaggc ccccccaagc
     7981 ctggtctaca ctgtggccac cagcacaacc ccacctgcag ccaccattct gcccaagggc
     8041 ccgccagccc ctgccactgc caccccagcc ccgactagcc ctttccccag cgccacagca
     8101 ggttccatga cctacagctt agtggccccc aaggcccagc ggcccagccc gaaggccccc
     8161 cagaaagtga aggcagccat cgccagcatt cccgtggggt cctttgaggc aggtgcctct
     8221 gggcggcctg gccctgcacc ccggcagcct ctggagcctg gcccagtccg agagccaact
     8281 gccccagagt ctgagcttga ggggcagccc acaccaccag cccctccacc cctgccagag
     8341 acctggactc ccacggcccg gagcagcccc ccactgcccc cacctgctga ggagcggacc
     8401 agcgccaagg gccctgagac catggccagc aaattcccca gctcatcttc agactggcgc
     8461 gtccctgggc agggcctgga gaatcgtggg gagcctccca ctcctcccag cccggcccca
     8521 gctccagctg tagcccctgg tggcagcagc gagagcagca gtgggcgggc agccggggac
     8581 accccggagc gcaaggaggc ggctggtact ggcaagaagg tgaaggtgcg gcccccgccc
     8641 ctgaagaaga cctttgactc tgtggacaac agggtcctgt cagaagtgga cttcgaagag
     8701 cgctttgctg agttgcctga gtttcggcct gaggaggtgc tgccctcccc caccctgcag
     8761 tctctggcca cctcaccccg ggccatcctg ggctcttacc gcaagaagag gaagaactcc
     8821 acggacctgg attcagcacc cgaggacccc acctcgccca agcgcaagat gagaagacgc
     8881 tccagctgca gctcggagcc caacaccccc aagagtgcca agtgcgaggg ggacatcttc
     8941 acctttgacc gtacaggtac agaagccgag gacgtgcttg gggagctaga gtatgacaag
     9001 gtgccatact cctccctgcg gcgcaccctg gaccagcgcc gggccctggt catgcagctc
     9061 tttcaggacc atggcttctt cccgtcagcc caggccacag ccgccttcca ggcccgctat
     9121 gcagacatct ttccctccaa ggtttgtctg cagttgaaga tccgtgaggt gcgccagaag
     9181 atcatgcagg ctgccactcc cacggagcag ccccctggag ctgaggctcc tctccctgta
     9241 ccgcccccca ctggcaccgc tgctgcccct gcccccactc ccagccccgc agggggccct
     9301 gaccccacct cacccagctc ggactctggc acggcccagg ctgccccgcc actgcctcca
     9361 cccccagagt cggggcctgg acagcctggc tgggaggggg ctccccagcc ctccccccca
     9421 cccccaggtc cctccacagc tgccacaggc ggaatctctg atgcaaatga ccatgatcta
     9481 cgtgagcctt ctggtatcaa gaatcctgga ggagccagcg cctctgcgag ggtttcatcc
     9541 tgggattctc agtcatatga cattgaacag atatgtctgg gggcttcaaa tcctccttgg
     9601 gcctccactc tctttgaaat agatgaattt gtaaagatct atgacttgcc aggggaagat
     9661 gacaccagca gcctaaatca atatcttttt ccagtatgcc ttgagtatga ccagctccaa
     9721 tcttcagtgt aaaacccagc tttcttgtac aaagtggtta ccggttagta atgaactagt
     9781 taagtcgaca atcaacgcgt taagtcgaca atcaacctct ggattacaaa atttgtgaaa
     9841 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     9901 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     9961 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
    10021 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
    10081 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
    10141 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
    10201 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
    10261 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
    10321 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
    10381 tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct
    10441 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
    10501 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
    10561 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
    10621 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
    10681 gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta
    10741 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
    10801 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
    10861 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
    10921 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
    10981 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
    11041 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
    11101 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
    11161 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
    11221 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
    11281 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
    11341 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
    11401 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
    11461 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
    11521 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
    11581 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
    11641 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
    11701 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
    11761 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
    11821 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
    11881 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
    11941 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
    12001 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
    12061 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
    12121 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
    12181 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
    12241 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
    12301 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
    12361 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
    12421 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
    12481 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
    12541 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
    12601 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
    12661 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
    12721 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
    12781 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
    12841 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
//
