LOCUS       sequence-491594-        8507 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Modified Re-NF-FF controller with degradation-tagged excisionase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8507)
  AUTHORS   Zhang R, Zhang R, Tian XJ
  TITLE     Integrating recombinase-based feedback and feedforward control for 
            optimal resource decoupling.
  JOURNAL   Nucleic Acids Res. 2025 Sep 5;53(17):gkaf913. doi: 
            10.1093/nar/gkaf913.
  PUBMED    40966512
REFERENCE   2  (bases 1 to 8507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2025-09-5"; volume: "53(17):gkaf913. doi"; 
            pages: " 10.1093/nar/gkaf913"
COMMENT     Sequence Label: Circuit C59
FEATURES             Location/Qualifiers
     source          1..8507
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             865..876
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             complement(888..1565)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPYDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     RBS             1572..1583
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      1932..2003
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2019..2046
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        2115..2149
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     RBS             2158..2169
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2176..2889
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(2206..2234)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2829..2849
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      2912..2983
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2999..3026
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        3041..3075
                     /label=BBa_J23110
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.33"
     RBS             3084..3095
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             3102..3980
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     terminator      4002..4073
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4089..4116
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        4131..4165
                     /label=BBa_J23105
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.24"
     CDS             4194..5696
                     /codon_start=1
                     /transl_table=11
                     /gene="Bxb1"
                     /product="Bxb1 integrase"
                     /label=Bxb1 integrase
                     /note="Bxb1 large serine integrase from Mycobacterium 
                     bacteriophage Bxb1 (Ghosh et al. 2003)."
                     /protein_id="AFF61396.1"
                     /translation="MRALVVIRLSRVTDATTSPERQLEYCQQLCAQRGWDVVGVAEDLD
                     VSGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKLV
                     VSATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWGY
                     LPTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQG
                     REPQGREWSATALKRSMISEAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRAE
                     LVKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGTV
                     AMAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLVGSPAYRAG
                     SPQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRSM
                     NVRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMS"
     terminator      5715..5786
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5802..5829
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    5836..5856
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             complement(5996..6811)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5996..6811)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6719..6738
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6803..6822
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      complement(7406..7950)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(7485..7504)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8418..8436
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(8440..8483)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    8486..8507
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 gcgaaaaaac cccgccgaag cggggttttt tgcgtactag agttattatt tagcagcagc
       61 gtagttttcg tcgtttgctg caccggtgac ttcccagacc tggccgtcaa cggtgaactt
      121 gcctcccagg atcgggacga tctcagcctt gacatgcttg ccgtcgaccg tgagcatccc
      181 gaagcccatc tgccagttcc cggctccgcc ctttagatag ttggccttct tcatgtccat
      241 caggtgcccg acttccatgc cggtgacggt cttgcgcacc gagccgccgt acccgaacga
      301 gtgcgagacg acagcctgcc ggtgcgtgtg gccgcagacc acggacttgc cgaacttctt
      361 ggcaccgttg agcgctgtcg atccggcgat ctgggatagc gtcatcttgc ccatgtgccc
      421 gtgagtggag atccagcccg gagcgatgtc gtagaagtca ggcagcagct ccacaccgaa
      481 cccgtcgaag tcgagcagca cgtcgatgtc gaaagcgtgc gtaccctcca gggccggtgc
      541 gttcttggcc aggtactcgc gggctcgcag atcgtggttg ccctcgtgca tcccgatcca
      601 gccgtcgtag accttgcgca gcggctccat caggttcttc ttggcgtagt ccgcgtcgcg
      661 gtacaccgag ccctcgaact cgcccttggt tccccggttc cagcgcgagg gctgcggcag
      721 gtctaggacg tcaccgatat gtaccacgcc gtacggctgg acatccccga tgaagcggat
      781 gaccgcttgc atctctttgc gcgcctcgaa aggcaactga gtatcgggta gaaagacgat
      841 acgctgagtg ccggccatct agtatttctc ctcttttact agagttatta agcaccggtg
      901 gagtgacgac cttcagcacg ttcgtactgt tcaacgatgg tgtagtcttc gttgtgggag
      961 gtgatgtcca gtttgatgtc ggttttgtaa gcacccggca gctgaaccgg ttttttagcc
     1021 atgtaggtgg ttttaacttc agcgtcgtag tgaccaccgt ctttcagttt cagacgcatt
     1081 ttgatttcac ctttcagagc accgtcttcc gggtacatac gttcggtgga agcttcccaa
     1141 cccatggttt ttttctgcat aaccggaccg tcgtacggga agttggtacc acgcagttta
     1201 actttgtaga tgaactcacc gtcttgcagg gaggagtcct gggtaacggt aacaacacca
     1261 ccgtcttcga agttcataac acgttcccat ttgaaacctt cagggaagga cagtttcagg
     1321 tagtccggga tgtcagccgg gtgtttaacg taagctttgg aaccgtactg gaactgcggg
     1381 gacaggatgt cccaagcgaa cggcagcgga ccacctttgg taactttcag tttagcggtc
     1441 tgggtacctt cgtacggacg accttcacct tcaccttcga tttcgaactc gtgaccgtta
     1501 acggaacctt ccatacgaac tttgaaacgc atgaactctt tgataacgtc ttcggaggaa
     1561 gccatctagt atttctcctc ttttactaga gctgccggct tgtcgacgac ggcgcactca
     1621 gtggtgtacg gtacaaaccc cgacaatagc gaggaggtaa attgtaagct ttagggaaac
     1681 gacatttact gacgcgactt tctcaaagct agggtgttgg cctgaactgt acgcttggag
     1741 ttgtaggaca gacataaaag tatcagatac gcacactaac catctgctag cccaaaaaaa
     1801 cggtatggag aaacagtaga gagttgcgat aaaaagcgtc aggtaggatc cgctaatctt
     1861 atggactatc ttgctatggc atagcaaagt gtgacgccgt gcaaataatc aatgtctcta
     1921 gtaccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     1981 tttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     2041 tctgcgttta tagcccggat gatcctgacg acggagtgcg cggtggttga ccagacaaac
     2101 cacgactact agagtttacg gctagctcag tcctaggtat agtgctagct actagagaaa
     2161 gaggagaaat actagatgcg taaaggagaa gaacttttca ctggagttgt cccaattctt
     2221 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt
     2281 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt
     2341 ccatggccaa cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca
     2401 gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa
     2461 agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa
     2521 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac
     2581 attcttggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac
     2641 aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc
     2701 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta
     2761 ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga
     2821 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa
     2881 ctatacaaat aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     2941 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     3001 ctcaccttcg ggtgggcctt tctgcgttta tatactagag tttacggcta gctcagtcct
     3061 aggtacaatg ctagctacta gagaaagagg agaaatacta gatggctgaa gcgcaaaatg
     3121 atcccctgct gccgggatac tcgtttaacg cccatctggt ggcgggttta acgccgattg
     3181 aggccaatgg ttatctcgat ttttttatcg accgaccgct gggaatgaaa ggttatattc
     3241 tcaatctcac cattcgcggt cagggggtgg tgaaaaatca gggacgagaa tttgtctgcc
     3301 gaccgggtga tattttgctg ttcccgccag gagagattca tcactacggt cgtcatccgg
     3361 aggctcgcga atggtatcac cagtgggttt actttcgtcc gcgcgcctac tggcatgaat
     3421 ggcttaactg gccgtcaata tttgccaata cgggtttctt tcgcccggat gaagcgcacc
     3481 agccgcattt cagcgacctg tttgggcaaa tcattaacgc cgggcaaggg gaagggcgct
     3541 attcggagct gctggcgata aatctgcttg agcaattgtt actgcggcgc atggaagcga
     3601 ttaacgagtc gctccatcca ccgatggata atcgggtacg cgaggcttgt cagtacatca
     3661 gcgatcacct ggcagacagc aattttgata tcgccagcgt cgcacagcat gtttgcctgt
     3721 cgccgtcgcg tctgtcacat cttttccgcc agcagttagg gattagcgtc ttaagctggc
     3781 gcgaggacca acgcatcagc caggcgaagc tgcttttgag cactacccgg atgcctatcg
     3841 ccaccgtcgg tcgcaatgtt ggttttgacg atcaactcta tttctcgcga gtatttaaaa
     3901 aatgcaccgg ggccagcccg agcgagttcc gtgccggttg tgaagaaaaa gtgaatgatg
     3961 tagccgtcaa gttgtcataa taaactacta gagccaggca tcaaataaaa cgaaaggctc
     4021 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     4081 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag tttacggcta
     4141 gctcagtcct aggtactatg ctagctacta gagtcacaca ggaaacctac tagatgagag
     4201 ccctggtagt catccgcctg tcccgcgtca ccgatgctac gacttcaccg gagcgtcagc
     4261 tggagtattg ccagcagctc tgcgcccagc gcggctggga cgtcgtcggg gtagcggagg
     4321 atctggacgt ctccggggcg gtcgatccgt tcgaccggaa gcgcagaccg aacctggccc
     4381 ggtggctagc gttcgaggag caaccgtttg acgtgatcgt ggcgtaccgg gtagatcggt
     4441 tgacccgatc gatccggcat cttcagcagc tggtccactg ggccgaggac cacaagaagc
     4501 tggtcgtctc cgcgaccgaa gcgcacttcg atacgacgac gccgtttgcg gcggtcgtca
     4561 tcgcgcttat gggaacggtg gcgcagatgg aattagaagc gatcaaagag cggaaccgtt
     4621 cggctgcgca tttcaatatc cgtgccggga aataccgagg atccctgccg ccgtggggat
     4681 acctgcctac gcgcgtggac ggggagtggc ggctggtgcc ggaccctgtg cagcgagagc
     4741 gcatcctcga ggtgtatcac cgcgtcgtcg acaaccacga gccgctgcat ctggtggccc
     4801 acgacctgaa ccggcgtggt gtcctgtcgc cgaaggacta cttcgcgcag ctgcaaggcc
     4861 gcgagccgca gggccgggag tggtcggcta ccgcgctgaa gcgatcgatg atctccgagg
     4921 cgatgctcgg gtacgcgact ctgaacggta agaccgtccg agacgacgac ggagccccgc
     4981 tggtgcgggc tgagccgatc ctgacccgtg agcagctgga ggcgctgcgc gccgagctcg
     5041 tgaagacctc ccgggcgaag cccgcggtgt ctaccccgtc gctgctgctg cgggtgttgt
     5101 tctgcgcggt gtgcggggag cccgcgtaca agttcgccgg gggaggacgt aagcacccgc
     5161 gctaccgctg ccgctcgatg gggttcccga agcactgcgg gaacggcacg gtggcgatgg
     5221 ccgagtggga cgcgttctgc gaggagcagg tactggatct gctcggggac gcggagcgtc
     5281 tggagaaagt ctgggtagcg ggctcggact ccgcggtcga actcgcggag gtgaacgcgg
     5341 agctggtgga cctgacgtcg ctggtcggct ccccggccta ccgggcgggc tctccgcagc
     5401 gagaagcact ggatgcccgt attgcggcgc tggccgcgcg gcaagaggag ctggagggcc
     5461 tggaggctcg cccgtctggc tgggagtggc gcgagaccgg gcagcggttc ggggactggt
     5521 ggcgggagca ggacaccgcg gcaaagaaca cctggcttcg gtcgatgaac gttcggctga
     5581 cgttcgacgt ccgcggcggg ctgactcgca cgatcgactt cggggatctt caggagtacg
     5641 agcagcatct caggctcggc agcgtggtcg aacggctaca caccgggatg tcgtaataaa
     5701 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     5761 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggttggc
     5821 ctttctgcgt ttatatacta gtagcggccg ctgcaggctt cctcgctcac tgactcgctg
     5881 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatctcgagt
     5941 cccgtcaagt cagcgtaatg ctctgccagt gttacaacca attaaccaat tctgattaga
     6001 aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta tcgataccat
     6061 atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga
     6121 tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta
     6181 atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat
     6241 ccggtgagaa tggcaaaagc ttatgcattt ctttccagac ttgttcaaca ggccagccat
     6301 tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct
     6361 gagcgagacg aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca
     6421 accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt
     6481 ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat gcatcatcag
     6541 gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc
     6601 tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact
     6661 ctggcgcatc gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat
     6721 cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctgg
     6781 agcaagacgt ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag
     6841 cagacagttt tattgttcat gatgatatat ttttatcttg tgcaatgtaa catcagagat
     6901 tttgagacac aacgtggctt tgttgaataa atcgaacttt tgctgagttg aaggatcaga
     6961 tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa gcaaaagttc aaaatcacca
     7021 actggtccac ctacaacaaa gctctcatca accgtggctc cctcactttc tggctggatg
     7081 atggggcgat tcaggcctgg tatgagtcag caacaccttc ttcacgaggc agacctcagc
     7141 gctagcggag tgtatactgg cttactatgt tggcactgat gagggtgtca gtgaagtgct
     7201 tcatgtggca ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg atacaggata
     7261 tattccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa
     7321 atggcttacg aacggggcgg agatttcctg gaagatgcca ggaagatact taacagggaa
     7381 gtgagagggc cgcggcaaag ccgtttttcc ataggctccg ccccctgaca agcatcacga
     7441 aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     7501 tccccctggc ggctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat
     7561 tccgctgtta tggccgcgtt tgtctcattc cacgcctgac actcagttcc gggtaggcag
     7621 ttcgctccaa gctggactgt atgcacgaac cccccgttca gtccgaccgc tgcgccttat
     7681 ccggtaacta tcgtcttgag tccaacccgg aaagacatgc aaaagcacca ctggcagcag
     7741 ccactggtaa ttgatttaga ggagttagtc ttgaagtcat gcgccggtta aggctaaact
     7801 gaaaggacaa gttttggtga ctgcgctcct ccaagccagt tacctcggtt caaagagttg
     7861 gtagctcaga gaaccttcaa aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa
     7921 gagattacgc gcagaccaaa acgatctcaa gaagatcatc ttattaaggg gtctgacgct
     7981 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     8041 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     8101 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     8161 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     8221 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     8281 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     8341 tccgcctcca tccagtctat tccatggtgc cacctgacgt ctaagaaacc attattatca
     8401 tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa aaaaaatcct
     8461 tagctttcgc taaggatgat ttctggaatt cgcggccgct tctagag
//
