LOCUS       sequence-491597-        8611 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Re-NF controller.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8611)
  AUTHORS   Zhang R, Zhang R, Tian XJ
  TITLE     Integrating recombinase-based feedback and feedforward control for 
            optimal resource decoupling.
  JOURNAL   Nucleic Acids Res. 2025 Sep 5;53(17):gkaf913. doi: 
            10.1093/nar/gkaf913.
  PUBMED    40966512
REFERENCE   2  (bases 1 to 8611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2025-09-5"; volume: "53(17):gkaf913. doi"; 
            pages: " 10.1093/nar/gkaf913"
COMMENT     Sequence Label: Circuit  C44
FEATURES             Location/Qualifiers
     source          1..8611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..35
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     RBS             44..55
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             62..775
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(92..120)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     715..735
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      798..869
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      885..912
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        927..961
                     /label=BBa_J23110
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.33"
     RBS             970..981
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             988..1866
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     terminator      1888..1959
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1975..2002
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        2017..2051
                     /label=BBa_J23105
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.24"
     CDS             2080..3582
                     /codon_start=1
                     /transl_table=11
                     /gene="Bxb1"
                     /product="Bxb1 integrase"
                     /label=Bxb1 integrase
                     /note="Bxb1 large serine integrase from Mycobacterium 
                     bacteriophage Bxb1 (Ghosh et al. 2003)."
                     /protein_id="AFF61396.1"
                     /translation="MRALVVIRLSRVTDATTSPERQLEYCQQLCAQRGWDVVGVAEDLD
                     VSGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKLV
                     VSATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWGY
                     LPTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQG
                     REPQGREWSATALKRSMISEAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRAE
                     LVKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGTV
                     AMAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLVGSPAYRAG
                     SPQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRSM
                     NVRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMS"
     terminator      3601..3672
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3688..3715
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             4561..4572
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             complement(4584..5261)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPYDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     RBS             5268..5279
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      5628..5699
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5715..5742
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      5819..5890
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5906..5933
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    5940..5960
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             complement(6100..6915)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6100..6915)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6823..6842
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6907..6926
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      complement(7510..8054)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(7589..7608)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8522..8540
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(8544..8587)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    8590..8611
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 tttacggcta gctcagtcct aggtatagtg ctagctacta gagaaagagg agaaatacta
       61 gatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
      121 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
      181 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
      241 tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca
      301 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt
      361 caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt
      421 taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa
      481 attggaatac aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg
      541 aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga
      601 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
      661 cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct
      721 tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat acaaataata
      781 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
      841 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
      901 ggcctttctg cgtttatata ctagagttta cggctagctc agtcctaggt acaatgctag
      961 ctactagaga aagaggagaa atactagatg gctgaagcgc aaaatgatcc cctgctgccg
     1021 ggatactcgt ttaacgccca tctggtggcg ggtttaacgc cgattgaggc caatggttat
     1081 ctcgattttt ttatcgaccg accgctggga atgaaaggtt atattctcaa tctcaccatt
     1141 cgcggtcagg gggtggtgaa aaatcaggga cgagaatttg tctgccgacc gggtgatatt
     1201 ttgctgttcc cgccaggaga gattcatcac tacggtcgtc atccggaggc tcgcgaatgg
     1261 tatcaccagt gggtttactt tcgtccgcgc gcctactggc atgaatggct taactggccg
     1321 tcaatatttg ccaatacggg tttctttcgc ccggatgaag cgcaccagcc gcatttcagc
     1381 gacctgtttg ggcaaatcat taacgccggg caaggggaag ggcgctattc ggagctgctg
     1441 gcgataaatc tgcttgagca attgttactg cggcgcatgg aagcgattaa cgagtcgctc
     1501 catccaccga tggataatcg ggtacgcgag gcttgtcagt acatcagcga tcacctggca
     1561 gacagcaatt ttgatatcgc cagcgtcgca cagcatgttt gcctgtcgcc gtcgcgtctg
     1621 tcacatcttt tccgccagca gttagggatt agcgtcttaa gctggcgcga ggaccaacgc
     1681 atcagccagg cgaagctgct tttgagcact acccggatgc ctatcgccac cgtcggtcgc
     1741 aatgttggtt ttgacgatca actctatttc tcgcgagtat ttaaaaaatg caccggggcc
     1801 agcccgagcg agttccgtgc cggttgtgaa gaaaaagtga atgatgtagc cgtcaagttg
     1861 tcataataaa ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     1921 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     1981 ccttcgggtg ggcctttctg cgtttatata ctagagttta cggctagctc agtcctaggt
     2041 actatgctag ctactagagt cacacaggaa acctactaga tgagagccct ggtagtcatc
     2101 cgcctgtccc gcgtcaccga tgctacgact tcaccggagc gtcagctgga gtattgccag
     2161 cagctctgcg cccagcgcgg ctgggacgtc gtcggggtag cggaggatct ggacgtctcc
     2221 ggggcggtcg atccgttcga ccggaagcgc agaccgaacc tggcccggtg gctagcgttc
     2281 gaggagcaac cgtttgacgt gatcgtggcg taccgggtag atcggttgac ccgatcgatc
     2341 cggcatcttc agcagctggt ccactgggcc gaggaccaca agaagctggt cgtctccgcg
     2401 accgaagcgc acttcgatac gacgacgccg tttgcggcgg tcgtcatcgc gcttatggga
     2461 acggtggcgc agatggaatt agaagcgatc aaagagcgga accgttcggc tgcgcatttc
     2521 aatatccgtg ccgggaaata ccgaggatcc ctgccgccgt ggggatacct gcctacgcgc
     2581 gtggacgggg agtggcggct ggtgccggac cctgtgcagc gagagcgcat cctcgaggtg
     2641 tatcaccgcg tcgtcgacaa ccacgagccg ctgcatctgg tggcccacga cctgaaccgg
     2701 cgtggtgtcc tgtcgccgaa ggactacttc gcgcagctgc aaggccgcga gccgcagggc
     2761 cgggagtggt cggctaccgc gctgaagcga tcgatgatct ccgaggcgat gctcgggtac
     2821 gcgactctga acggtaagac cgtccgagac gacgacggag ccccgctggt gcgggctgag
     2881 ccgatcctga cccgtgagca gctggaggcg ctgcgcgccg agctcgtgaa gacctcccgg
     2941 gcgaagcccg cggtgtctac cccgtcgctg ctgctgcggg tgttgttctg cgcggtgtgc
     3001 ggggagcccg cgtacaagtt cgccggggga ggacgtaagc acccgcgcta ccgctgccgc
     3061 tcgatggggt tcccgaagca ctgcgggaac ggcacggtgg cgatggccga gtgggacgcg
     3121 ttctgcgagg agcaggtact ggatctgctc ggggacgcgg agcgtctgga gaaagtctgg
     3181 gtagcgggct cggactccgc ggtcgaactc gcggaggtga acgcggagct ggtggacctg
     3241 acgtcgctgg tcggctcccc ggcctaccgg gcgggctctc cgcagcgaga agcactggat
     3301 gcccgtattg cggcgctggc cgcgcggcaa gaggagctgg agggcctgga ggctcgcccg
     3361 tctggctggg agtggcgcga gaccgggcag cggttcgggg actggtggcg ggagcaggac
     3421 accgcggcaa agaacacctg gcttcggtcg atgaacgttc ggctgacgtt cgacgtccgc
     3481 ggcgggctga ctcgcacgat cgacttcggg gatcttcagg agtacgagca gcatctcagg
     3541 ctcggcagcg tggtcgaacg gctacacacc gggatgtcgt aataaactag agccaggcat
     3601 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     3661 gtgaacgctc tctactagag tcacactggc tcaccttcgg gttggccttt ctgcgtttat
     3721 atactagagg cgaaaaaacc ccgccgaagc ggggtttttt gcgtactaga gttattaacc
     3781 ggtgacttcc cagacctggc cgtcaacggt gaacttgcct cccaggatcg ggacgatctc
     3841 agccttgaca tgcttgccgt cgaccgtgag catcccgaag cccatctgcc agttcccggc
     3901 tccgcccttt agatagttgg ccttcttcat gtccatcagg tgcccgactt ccatgccggt
     3961 gacggtcttg cgcaccgagc cgccgtaccc gaacgagtgc gagacgacag cctgccggtg
     4021 cgtgtggccg cagaccacgg acttgccgaa cttcttggca ccgttgagcg ctgtcgatcc
     4081 ggcgatctgg gatagcgtca tcttgcccat gtgcccgtga gtggagatcc agcccggagc
     4141 gatgtcgtag aagtcaggca gcagctccac accgaacccg tcgaagtcga gcagcacgtc
     4201 gatgtcgaaa gcgtgcgtac cctccagggc cggtgcgttc ttggccaggt actcgcgggc
     4261 tcgcagatcg tggttgccct cgtgcatccc gatccagccg tcgtagacct tgcgcagcgg
     4321 ctccatcagg ttcttcttgg cgtagtccgc gtcgcggtac accgagccct cgaactcgcc
     4381 cttggttccc cggttccagc gcgagggctg cggcaggtct aggacgtcac cgatatgtac
     4441 cacgccgtac ggctggacat ccccgatgaa gcggatgacc gcttgcatct ctttgcgcgc
     4501 ctcgaaaggc aactgagtat cgggtagaaa gacgatacgc tgagtgccgg ccatctagta
     4561 tttctcctct tttactagag ttattaagca ccggtggagt gacgaccttc agcacgttcg
     4621 tactgttcaa cgatggtgta gtcttcgttg tgggaggtga tgtccagttt gatgtcggtt
     4681 ttgtaagcac ccggcagctg aaccggtttt ttagccatgt aggtggtttt aacttcagcg
     4741 tcgtagtgac caccgtcttt cagtttcaga cgcattttga tttcaccttt cagagcaccg
     4801 tcttccgggt acatacgttc ggtggaagct tcccaaccca tggttttttt ctgcataacc
     4861 ggaccgtcgt acgggaagtt ggtaccacgc agtttaactt tgtagatgaa ctcaccgtct
     4921 tgcagggagg agtcctgggt aacggtaaca acaccaccgt cttcgaagtt cataacacgt
     4981 tcccatttga aaccttccgg gaaggacagt ttcaggtagt ccgggatgtc agccgggtgt
     5041 ttaacgtaag ctttggaacc gtactggaac tgcggggaca ggatgtccca agcgaacggc
     5101 agcggaccac ctttggtaac tttcagttta gcggtctggg taccttcgta cggacgacct
     5161 tcaccttcac cttcgatttc gaactcgtga ccgttaacgg aaccttccat acgaactttg
     5221 aaacgcatga actctttgat aacgtcttcg gaggaagcca tctagtattt ctcctctttt
     5281 actagagcgg ccggcttgtc gacgacggcg cactcagtgg tgtacggtac aaaccccgac
     5341 aatagcgagg aggtaaattg taagctttag ggaaacgaca tttactgacg cgactttctc
     5401 aaagctaggg tgttggcctg aactgtacgc ttggagttgt aggacagaca taaaagtatc
     5461 agatacgcac actaaccatc tgctagccca aaaaaacggt atggagaaac agtagagagt
     5521 tgcgataaaa agcgtcaggt aggatccgct aatcttatgg actatcttgc tatggcatag
     5581 caaagtgtga cgccgtgcaa ataatcaatg tctctagtac caggcatcaa ataaaacgaa
     5641 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     5701 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatagc ccggatgatc
     5761 ctgacgacgg agtgcgcggt ggttgaccag acaaaccacg actactagag ccaggcatca
     5821 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     5881 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat
     5941 actagtagcg gccgctgcag gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     6001 tgcggcgagc ggtatcagct cactcaaagg cggtaatctc gagtcccgtc aagtcagcgt
     6061 aatgctctgc cagtgttaca accaattaac caattctgat tagaaaaact catcgagcat
     6121 caaatgaaac tgcaatttat tcatatcagg attatcgata ccatattttt gaaaaagccg
     6181 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
     6241 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
     6301 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
     6361 aagcttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
     6421 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac
     6481 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
     6541 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
     6601 tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
     6661 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
     6721 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
     6781 cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
     6841 cccatataaa tcagcatcca tgttggaatt taatcgcggc ctggagcaag acgtttcccg
     6901 ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt
     6961 tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg
     7021 gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc atcttcccga
     7081 caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggt ccacctacaa
     7141 caaagctctc atcaaccgtg gctccctcac tttctggctg gatgatgggg cgattcaggc
     7201 ctggtatgag tcagcaacac cttcttcacg aggcagacct cagcgctagc ggagtgtata
     7261 ctggcttact atgttggcac tgatgagggt gtcagtgaag tgcttcatgt ggcaggagaa
     7321 aaaaggctgc accggtgcgt cagcagaata tgtgatacag gatatattcc gcttcctcgc
     7381 tcactgactc gctacgctcg gtcgttcgac tgcggcgagc ggaaatggct tacgaacggg
     7441 gcggagattt cctggaagat gccaggaaga tacttaacag ggaagtgaga gggccgcggc
     7501 aaagccgttt ttccataggc tccgccccct gacaagcatc acgaaatctg acgctcaaat
     7561 cagtggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggcggctcc
     7621 ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg tcattccgct gttatggccg
     7681 cgtttgtctc attccacgcc tgacactcag ttccgggtag gcagttcgct ccaagctgga
     7741 ctgtatgcac gaaccccccg ttcagtccga ccgctgcgcc ttatccggta actatcgtct
     7801 tgagtccaac ccggaaagac atgcaaaagc accactggca gcagccactg gtaattgatt
     7861 tagaggagtt agtcttgaag tcatgcgccg gttaaggcta aactgaaagg acaagttttg
     7921 gtgactgcgc tcctccaagc cagttacctc ggttcaaaga gttggtagct cagagaacct
     7981 tcaaaaaacc gccctgcaag gcggtttttt cgttttcaga gcaagagatt acgcgcagac
     8041 caaaacgatc tcaagaagat catcttatta aggggtctga cgctcagtgg aacgaaaact
     8101 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     8161 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     8221 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     8281 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     8341 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     8401 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     8461 ctattccatg gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     8521 aaataggcgt atcacgaggc agaatttcag ataaaaaaaa tccttagctt tcgctaagga
     8581 tgatttctgg aattcgcggc cgcttctaga g
//
