LOCUS       sequence-49160-e         523 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 523)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 523)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PBRM1
FEATURES             Location/Qualifiers
     source          1..523
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             452..472
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             473..490
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             491..514
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cttaagaagg agatatacta tgggtgatag catgatcagc agcgcgacca gcgataccgg
       61 cagcgcgaaa cgtaaaagca aaaaaaatat ccgtaaacag cgcatgaaaa ttctgtttaa
      121 cgtggtgctg gaagcccgtg aaccgggcag cggtcgccgc ctgtgtgatc tgtttatggt
      181 gaaaccgagc aaaaaagatt atccggatta ttataaaatc atcctggaac cgatggatct
      241 gaaaattatt gaacacaata tccgtaacga taaatatgcc ggcgaagaag gtatgattga
      301 agatatgaaa ctgatgtttc gtaacgcccg tcactataac gaagaaggta gccaggtgta
      361 taatgatgcg catatcctgg aaaaactgct gaaagaaaaa cgcaaagaac tgggtccgct
      421 gccggatgat gatgatatgg cgagcccggc agagaacctc tacttccaat cgcaccatca
      481 tcaccaccat gattacaagg atgacgacga taagtgagga tcc
//
