LOCUS       sequence-49161-e        1417 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1417)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ENOSF1
FEATURES             Location/Qualifiers
     source          1..1417
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tggtgcgcgg caggatctcc cggctctcgg tccgggacgt gcgcttcccc
      121 acgtcgcttg ggggccacgg cgcggacgcc atgcacacgg accctgacta ctcggctgcc
      181 tatgtcgtca tagaaactga tgcagaagat ggaatcaagg ggtgtggaat taccttcact
      241 ctgggaaaag gcactgaagt tgttgtctgt gctgtgaatg ccctcgccca ccatgtgctc
      301 aacaaggacc tcaaggacat tgttggtgac ttcagaggct tctataggca gctcacaagt
      361 gatgggcagc tcagatggat tggtccagaa aagggcgtgg tgcacctggc gacagcggcc
      421 gtcctaaacg cggtgtggga cttgtgggcc aagcaggagg gaaagcctgt ctggaagtta
      481 cttgtggaca tggatcccag gatgctggta tcctgcatag atttcaggta catcactgat
      541 gtcctgactg aggaggatgc cctagaaata ctgcagaaag gtcaaattgg taaaaaagaa
      601 agagagaagc aaatgctggc acaaggatac cctgcttaca cgacatcgtg cgcctggctg
      661 gggtactcag atgacacgtt gaagcagctc tgtgcccagg cgctgaagga tggctggacc
      721 aggtttaaag taaaggtggg tgctgatctc caggatgaca tgcgaagatg ccaaatcatc
      781 cgagacatga ttggaccgga aaagactttg atgatggatg ccaaccagcg ctgggatgtg
      841 cctgaggcgg tggagtggat gtccaagctg gccaagttca agccattgtg gattgaggag
      901 ccaacctccc ctgatgacat tctggggcac gccaccattt ccaaggcact ggtcccatta
      961 ggaattggca ttgccacagg agaacagtgc cacaatagag tgatatttaa gcaactccta
     1021 caggcgaagg ccctgcagtt cctccagatt gacagttgca gactgggcag tgtcaatgag
     1081 aacctctcag tattgctgat ggccaaaaag tttgaaattc ctgtttgccc ccatgctggt
     1141 ggagttggcc tctgtgaact ggtgcagcac ctgattatat ttgactacat atcagtttct
     1201 gcaagccttg aaaatagggt gtgtgagtat gttgaccacc tgcatgagca tttcaagtat
     1261 cccgtgatga tccagcgggc ttcctacatg cctcccaagg atcccggcta ctcaacagaa
     1321 atgaaggagg aatctgtaaa gaaacaccag tatccagatg gtgaagtttg gaagaaactc
     1381 cttcctgctt gacagtaaag gtggatacgg atccgaa
//
