LOCUS       sequence-49162-e         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: AMPH
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tggccgacat caagacgggc atcttcgcca agaacgtcca gaagcgactc
      121 aaccgcgcgc aggaaaaggt cctccaaaag ctggggaaag ctgatgagac aaaagacgaa
      181 cagttcgaag aatatgtcca gaacttcaaa cggcaagaag cagagggtac cagacttcag
      241 cgagaactcc gaggatattt agcagcaatc aaaggcatgc aggaggcctc catgaagctc
      301 acagagtcgc tgcatgaagt ctatgagcct gactggtatg ggcgggaaga tgtgaaaatg
      361 gttggtgaga aatgtgatgt gctgtgggaa gacttccatc aaaaactcgt ggatgggtcc
      421 ttgctaacac tggataccta cctggggcaa tttcctgaca taaagaatcg catcgccaag
      481 cgcagcagga agctagtgga ctatgacagt gcccgccacc atctggaagc tctgcagagc
      541 tccaagagga aggatgagag tcgaatctct aaggcagaag aagaatttca gaaagcacag
      601 aaagtgtttg aagagtttaa cgttgactta caagaagagt taccatcatt atggtcaaga
      661 cgagttggat tttatgttaa tactttcaaa aacgtctcca gccttgaagc caagtttcat
      721 aaggaaattg cggtgctttg ccacaaactg tatgaagtga tgacaaaact gggtgaccag
      781 cacgccgaca aggcctgaca gtaaaggtgg atacggatcc gaa
//
