LOCUS       sequence-49165-e        1373 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1373)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: CUL4B
FEATURES             Location/Qualifiers
     source          1..1373
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             31..201
                     /codon_start=1
                     /product="Z domain of staphylococcal protein A, engineered 
                     to contain multiple positive charges (Hedhammar and Hober, 
                     2007)"
                     /label=Z-basic tag
                     /note="useful for purifying tagged proteins by 
                     cation-exchange chromatography"
                     /translation="VDNKFNKERRRARREIRHLPNLNREQRRAFIRSLRDDPSQSANLL
                     AEAKKLNDAQPK"
     CDS             208..228
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1353..1370
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtggataaca agttcaacaa ggagcgtcga
       61 agagctcgcc gtgaaattcg ccatctgccg aacctgaacc gcgaacagcg tcgcgcattt
      121 attcgcagcc tgcgcgatga tccgagccag agcgcgaacc tgctggcgga agcgaagaag
      181 ctgaacgatg cgcagccgaa gggtaccgag aacctgtact tccaatccat gccgaagctg
      241 cctgagaatt ataccgatga aacctggcag aaactgaagg aagccgtgga agcaatccag
      301 aacagtacct cgattaaata caatctggaa gagctgtatc aggccgtcga gaacctgtgc
      361 tcctacaaaa tctctgctaa tctgtataag cagctgcgtc agatttgtga ggaccacatc
      421 aaggcgcaga ttcatcagtt ccgcgaagac agcctggatt ccgtgctgtt tctgaagaaa
      481 atcgatcgtt gctggcagaa ccactgtcgt cagatgatca tgattcgctc aattttcctg
      541 tttctggacc gcacctacgt cctgcagaac agcatgctgc cgagtatctg ggatatgggt
      601 ctggaactgt tccgtgccca tatcatttct gaccagaaag ttcagaacaa gaccatcgat
      661 ggtattctgc tgctgatcga gcgtgaacgc aatggcgagg ctattgaccg ttccctgctg
      721 cgctctctgc tgtcaatgct gagtgacctg cagatttacc aggattcttt cgaacagcgt
      781 tttctggaag agaccaaccg cctgtatgcc gctgagggcc agaaactgat gcaggagcgc
      841 gaagttcctg aatacctgca ccatgtgaat aagcgtctgg aagaggaagc cgaccgcctg
      901 atcacctacc tggatcagac cacccagaaa agcctgattg cgaccgtgga gaagcagctg
      961 ctgggtgaac acctgaccgc aatcctgcag aaaggcctga acaatctgct ggacgagaac
     1021 cgtattcagg atctgtcgct gctgtatcag ctgttcagcc gtgtccgcgg tggcgtgcag
     1081 gtcctgctgc agcagtggat cgaatacatt aaggcgtttg gcagcaccat cgttattaac
     1141 ccggagaaag acaagaccat gcgccaggaa ctggacgatt tcaaagacaa ggtggatcat
     1201 atcattgata tctgcttcct gaagaacgaa aagttcatca acgccatgaa ggaagccttc
     1261 gaaaccttta tcaacaaacg tcccaattga cagtaaaggt ggatacggat ccgaattcga
     1321 gctccgtcga caagcttgcg gccgcactcg agcaccacca ccaccaccac tga
//
