LOCUS       sequence-49166-e        1006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1006)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: CDKL1
FEATURES             Location/Qualifiers
     source          1..1006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             935..955
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             956..973
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             974..997
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cttaagaagg agatatacta tgatggagaa gtatgaaaaa attgggaaaa ttggagaagg
       61 ctcctatgga gttgttttca aatgtagaaa cagggacacg ggtcagattg tggccatcaa
      121 gaagtttctg gaatcagaag atgaccctgt cataaagaaa attgcccttc gggaaatccg
      181 aatgctcaag caactcaagc atcccaacct tgttaacctc ctggaagtct tcaggaggaa
      241 acggaggctt cacctggtgt ttgaatattg tgaccacaca gttctccatg agttggacag
      301 ataccaaaga ggggtaccag aacatctcgt gaagagcata acttggcaga cactgcaagc
      361 tgtaaatttt tgccataaac acaattgcat acatagagac gtgaagccag aaaatatcct
      421 catcacgaaa cattccgtga ttaagctttg tgactttgga tttgctcggc ttttgactgg
      481 accgagtgac tactatgacg acgaagtggc taccaggtgg taccgctccc ctgagctgct
      541 ggtgggggac acgcagtacg gccccccggt ggatgtttgg gcaattggct gtgtctttgc
      601 tgagctgctg tcaggagtgc ctctgtggcc aggaaaatcg gatgtggatc agctgtatct
      661 gattaggaag accttggggg atctcattcc taggcaccag caagtgttta gcacgaatca
      721 gtacttcagt ggagtgaaaa ttccagaccc tgaagatatg gaaccacttg aattaaaatt
      781 cccaaacatc tcttatcctg ccctggggct cctaaagggc tgtctccaca tggaccctac
      841 tgaaaggctg acatgtgaac agctgttgca tcacccatat tttgaaaaca tcagagaaat
      901 agaggatttg gcaaaagaac acgacaaacc agcagagaac ctctacttcc aatcgcacca
      961 tcatcaccac catgattaca aggatgacga cgataagtga ggatcc
//
