LOCUS       sequence-491666-       11474 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a synNotch receptor binding to BCAN.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11474)
  AUTHORS   Simic MS, Watchmaker PB, Gupta S, Wang Y, Sagan SA, Duecker J, 
            Shepherd C, Diebold D, Pineo-Cavanaugh P, Haegelin J, Zhu R, Ng B, 
            Yu W, Tonai Y, Cardarelli L, Reddy NR, Sidhu SS, Troyanskaya O, 
            Hauser SL, Wilson MR, Zamvil SS, Okada H, Lim WA
  TITLE     Programming tissue-sensing T cells that deliver therapies to the 
            brain.
  JOURNAL   Science. 2024 Dec 6;386(6726):eadl4237. doi: 
            10.1126/science.adl4237. Epub 2024 Dec 6.
  PUBMED    39636984
REFERENCE   2  (bases 1 to 11474)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2024-12-6"; volume: "386(6726):eadl4237. doi"; pages: " 
            10.1126/science.adl4237. Epub 2024 Dec 6"
COMMENT     Sequence Label: pHR_PGK_ gb128_ BCAN 13138 VLVH synNotch
FEATURES             Location/Qualifiers
     source          1..11474
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..17)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             107..340
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(365..383)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(366..383)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(673..692)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(906..925)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1025..1047
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1085..1103)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1171..1275
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1276..1344,1345..2136)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1494..1513)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2307..2895
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2796..2815
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3049..3066
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3136..3156)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3141..3470
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3321..3456
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4604..4669
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4799..4819
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5244..5378
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5281..5300)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5335..5354
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5546..6179
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6226..6351
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6848..7081
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7266..7310
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7595..7712
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7772..8271
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7807..7830)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8204..8223
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      8320..8329
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8389..8418
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             10138..10578
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             10615..10764
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     misc_feature    10883..11471
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10936..10956)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11354..11365)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(join(11474,1..16))
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gataccgtcg acctcgaggg aattaattcg agctcggtac ctttaagacc aatgacttac
       61 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
      121 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
      181 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
      241 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
      301 tccctcagac ccttttagtc agtgtggaaa atctctagca gcatctagaa ttaattccgt
      361 gtattctata gtgtcaccta aatcgtatgt gtatgataca taaggttatg tattaattgt
      421 agccgcgttc taacgacaat atgtacaagc ctaattgtgt agcatctggc ttactgaagc
      481 agaccctatc atctctctcg taaactgccg tcagagtcgg tttggttgga cgaaccttct
      541 gagtttctgg taacgccgtc ccgcacccgg aaatggtcag cgaaccaatc agcagggtca
      601 tcgctagcca gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg
      661 cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg
      721 ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt
      781 tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc
      841 tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga atggtgcact
      901 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
      961 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     1021 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     1081 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     1141 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     1201 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     1261 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     1321 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     1381 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     1441 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     1501 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     1561 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     1621 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     1681 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     1741 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     1801 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     1861 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     1921 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     1981 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     2041 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     2101 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     2161 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     2221 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     2281 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     2341 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     2401 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     2461 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     2521 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     2581 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     2641 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     2701 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     2761 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     2821 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     2881 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     2941 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     3001 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     3061 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     3121 cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     3181 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     3241 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     3301 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     3361 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     3421 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttggac
     3481 acaagacagg cttgcgagat atgtttgaga ataccacttt atcccgcgtc agggagaggc
     3541 agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatctctat
     3601 aatctcgcgc aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg
     3661 acacttcaca tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt
     3721 aaactcgcaa gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt
     3781 ggcggtctgt accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg
     3841 tttgtatttc cagctacgat cacgacaacc agcgcgagct taaagtgctg aaacgcgcag
     3901 aaggcgatgg cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgcgg
     3961 ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg
     4021 gtcgtccgct ggttgatgac tatgttgttg atatcccgca agatacctgg attgaacagc
     4081 cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc
     4141 tggcactgcc gggcgttgtt ctttttaact tcaggcgggt tacaatagtt tccagtaagt
     4201 attctggagg ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc
     4261 tttaaacatc ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct
     4321 gtgcagtcgg cccttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg
     4381 atgtggatct ggcgcggcat tgacccacgc gaaatcctcg acgtccaggc acgtattgtg
     4441 atgagcgatg ccgaacgtac cgacgatgat ttatacgata cggtgattgg ctaccgtggc
     4501 ggcaactgga tttatgagtg ggccccggat ctttgtgaag gaaccttact tctgtggtgt
     4561 gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt
     4621 aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta
     4681 tggaactgat gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc
     4741 agaagaaatg ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc
     4801 aaaaaagaag agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt
     4861 gagtcatgct gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga
     4921 aaaagctgca ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag
     4981 gcataacagt tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc
     5041 tgctattaat aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt
     5101 taataaggaa tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat
     5161 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     5221 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     5281 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     5341 tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc
     5401 aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc
     5461 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
     5521 aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat
     5581 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca
     5641 ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     5701 gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg
     5761 agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc
     5821 ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat
     5881 cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg
     5941 actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg
     6001 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     6061 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     6121 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     6181 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     6241 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     6301 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     6361 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     6421 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     6481 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     6541 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     6601 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     6661 aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag
     6721 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     6781 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     6841 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     6901 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     6961 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     7021 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     7081 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     7141 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     7201 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     7261 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     7321 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     7381 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     7441 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
     7501 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatctc
     7561 gacggtatcg ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     7621 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     7681 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     7741 cagtttggat cgataagctt gatatcgaat tgggtagggg aggcgctttt cccaaggcag
     7801 tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg
     7861 cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc
     7921 ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg
     7981 tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc
     8041 accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc
     8101 ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg
     8161 ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa
     8221 aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc gaattctcac
     8281 gcgtcaagtg gagcaaggca ggtggacagt ggatccgccg ccaccatggc gttgcccgtg
     8341 acggcactgt tgctcccact cgcgctgctg ctgcacgctg caaggcctga acaaaaactt
     8401 atcagcgaag aggatctgga tatccagatg acccagtccc cgagctccct gtccgcctct
     8461 gtgggcgata gggtcaccat cacctgccgt gccagtcagt ccgtgtccag cgctgtagcc
     8521 tggtatcaac agaaaccagg aaaagctccg aagcttctga tttactcggc atccgatctc
     8581 tactctggag tcccttctcg cttctctggt agccgttccg ggacggattt cactctgacc
     8641 atcagcagtc tgcagccgga agacttcgca acttattact gtcagcaatt ctactatctg
     8701 ataacgttcg gacagggtac caaggtggag atcaaaggta ctactgccgc tagtggtagt
     8761 agtggtggca gtagcagtgg tgccgaggtt cagctggtgg agtctggcgg tggcctggtg
     8821 cagccagggg gctcactccg tttgtcctgt gcagcttctg gcttcgatat ttcttcttat
     8881 tctatccact gggtgcgtca ggccccgggt aagggcctgg aatgggttgc aggcataagt
     8941 tctagcgggg gagacacagc atatgccgat agcgtcaagg gccgtttcac tataagcgca
     9001 gacacatcca aaaacacagc ctacctacaa atgaacagct taagagctga ggacactgcc
     9061 gtctattatt gtgctcgcta tttctacagt catagtcggt atgcctactc aggtgcgctc
     9121 gactactggg gtcaaggaac cctggtcacc gtctcctcga tcctggacta cagcttcaca
     9181 ggtggcgctg ggcgcgacat tcccccaccg cagattgagg aggcctgtga gctgcctgag
     9241 tgccaggtgg atgcaggcaa taaggtctgc aacctgcagt gtaataatca cgcatgtggc
     9301 tgggatggtg gcgactgctc cctcaacttc aatgacccct ggaagaactg cacgcagtct
     9361 ctacagtgct ggaagtattt tagcgacggc cactgtgaca gccagtgcaa ctcggccggc
     9421 tgcctctttg atggcttcga ctgccagctc accgagggac agtgcaaccc cctgtatgac
     9481 cagtactgca aggaccactt cagtgatggc cactgcgacc agggctgtaa cagtgccgaa
     9541 tgtgagtggg atggcctaga ctgtgctgag catgtacccg agcggctggc agccggcacc
     9601 ctggtgctgg tggtgctgct tccacccgac cagctacgga acaactcctt ccactttctg
     9661 cgggagctca gccacgtgct gcacaccaac gtggtcttca agcgtgatgc gcaaggccag
     9721 cagatgatct tcccgtacta tggccacgag gaagagctgc gcaagcaccc aatcaagcgc
     9781 tctacagtgg gttgggccac ctcttcactg cttcctggta ccagtggtgg gcgccagcgc
     9841 agggagctgg accccatgga catccgtggc tccattgtct acctggagat cgacaaccgg
     9901 caatgtgtgc agtcatcctc gcagtgcttc cagagtgcca ccgatgtggc tgccttccta
     9961 ggtgctcttg cgtcacttgg cagcctcaat attccttaca agattgaggc cgtgaagagt
    10021 gagccggtgg agcctccgct gccctcgcag ctgcacctca tgtacgtggc agcggccgcc
    10081 ttcgtgctcc tgttctttgt gggctgtggg gtgctgctgt cccgcaagcg ccggcggatg
    10141 aagctgctga gcagcatcga gcaggcctgt gacatctgcc ggctgaagaa actgaagtgc
    10201 agcaaagaaa agcccaagtg cgccaagtgc ctgaagaaca actgggagtg ccggtacagc
    10261 cccaagacca agagaagccc cctgaccaga gcccacctga ccgaggtgga aagccggctg
    10321 gaaagactgg aacagctgtt tctgctgatc ttcccacgcg aggacctgga catgatcctg
    10381 aagatggaca gcctgcagga catcaaggcc ctgctgaccg gcctgttcgt gcaggacaac
    10441 gtgaacaagg acgccgtgac cgacagactg gccagcgtgg aaaccgacat gcccctgacc
    10501 ctgcggcagc acagaatcag cgccaccagc agcagcgagg aaagcagcaa caagggccag
    10561 cggcagctga cagtgtctgc tgctgcaggc ggaagcggag gctctggcgg atctgatgcc
    10621 ctggacgact tcgacctgga tatgctgggc agcgacgccc tggatgattt tgatctggac
    10681 atgctgggat ctgacgctct ggacgatttc gatctcgaca tgttgggatc agatgcactg
    10741 gatgactttg acctggacat gctcggatca tgagatcctt gacttgcggc cgcaactccc
    10801 acctgcaaca tgcgtgactg actgaggccg cgactctaga gtcgacctgc aggcatgcaa
    10861 gcttgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac
    10921 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
    10981 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
    11041 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
    11101 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
    11161 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
    11221 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
    11281 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
    11341 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
    11401 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
    11461 cgcctccccg catc
//
