LOCUS       sequence-49167-e        1177 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1177)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1177)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ELAC1
FEATURES             Location/Qualifiers
     source          1..1177
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1106..1126
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1127..1144
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1145..1168
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cttaagaagg agatatacta tggatgtgac attcctgggg acgggtgcag catacccatc
       61 tccaacccgg ggtgcctctg ctgtggtcct tcggtgtgaa ggcgagtgct ggctctttga
      121 ctgtggggag ggaacacaga cacagcttat gaaaagccaa cttaaagcag ggagaattac
      181 caagatcttc atcacacacc ttcatggaga ccatttcttt ggccttcctg ggctcctctg
      241 cacaatcagc ctgcagagtg gctccatggt gtccaaacag cctattgaaa tctatggccc
      301 tgtagggctt cgggacttta tctggcgaac catggaactc tctcacacgg agctggtctt
      361 ccattatgtg gttcatgaac tggttcctac agcagatcaa tgtcctgcag aagaactaaa
      421 agaatttgcg catgtgaata gagcagacag tcctcccaaa gaggaacaag gaagaactat
      481 cctgttagac tcagaagaaa actcatacct tctgtttgat gatgaacaat ttgttgtaaa
      541 agcatttcgc ctctttcaca gaattccctc atttgggttt tcagtcgtgg aaaagaaacg
      601 cccaggtaaa ctcaatgcac agaaacttaa agaccttggt gttccaccag gtcctgccta
      661 tgggaagctg aaaaatggaa tttctgttgt tctggaaaat ggggttacaa tttctcccca
      721 agatgtctta aaaaagccta ttgttggaag aaaaatctgc atattgggtg actgctctgg
      781 ggttgtgggt gatggaggag taaaactgtg ctttgaagca gacctgttga tccacgaagc
      841 aaccctggat gatgcccaga tggacaaagc aaaggagcat ggccacagca caccacagat
      901 ggcagcaaca tttgcaaagt tgtgccgtgc aaagaggctg gttctgactc acttcagtca
      961 gaggtacaaa ccagttgcct tggccagaga aggagaaaca gatggcattg cagaactaaa
     1021 aaagcaagct gaatcagtgt tagatctcca agaagtgact ctagcagaag attttatggt
     1081 gataagcatt ccaatcaaga aagcagagaa cctctacttc caatcgcacc atcatcacca
     1141 ccatgattac aaggatgacg acgataagtg aggatcc
//
