LOCUS       sequence-491671-       11491 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a synNotch receptor binding to GRM3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11491)
  AUTHORS   Simic MS, Watchmaker PB, Gupta S, Wang Y, Sagan SA, Duecker J, 
            Shepherd C, Diebold D, Pineo-Cavanaugh P, Haegelin J, Zhu R, Ng B, 
            Yu W, Tonai Y, Cardarelli L, Reddy NR, Sidhu SS, Troyanskaya O, 
            Hauser SL, Wilson MR, Zamvil SS, Okada H, Lim WA
  TITLE     Programming tissue-sensing T cells that deliver therapies to the 
            brain.
  JOURNAL   Science. 2024 Dec 6;386(6726):eadl4237. doi: 
            10.1126/science.adl4237. Epub 2024 Dec 6.
  PUBMED    39636984
REFERENCE   2  (bases 1 to 11491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2024-12-6"; volume: "386(6726):eadl4237. doi"; pages: " 
            10.1126/science.adl4237. Epub 2024 Dec 6"
COMMENT     Sequence Label: pHR_PGK_ GRM3 13144 VLVH synNotch
FEATURES             Location/Qualifiers
     source          1..11491
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        137..636
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(172..195)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     569..588
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      684..693
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             753..782
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2520..2960
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             2997..3146
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     misc_feature    3265..3853
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3318..3338)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3736..3747)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3856..3872)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3857..3873)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3963..4196
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4221..4239)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4222..4239)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4529..4548)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4762..4781)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4881..4903
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4941..4959)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5027..5131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5132..5200,5201..5992)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5350..5369)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6163..6751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6652..6671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6905..6922
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6992..7012)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6997..7326
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7177..7312
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8460..8525
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8655..8675
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9100..9234
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9137..9156)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9191..9210
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9402..10035
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10082..10207
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10704..10937
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11122..11166
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    join(11451..11491,1..77)
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
       61 caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt tggatcgata
      121 agcttgatat cgaattgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct
      181 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc
      241 acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct
      301 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga
      361 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg
      421 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct
      481 cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg
      541 ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc
      601 gcgctgttct cctcttcctc atctccgggc ctttcgaatt ctcacgcgtc aagtggagca
      661 aggcaggtgg acagtggatc gccgccacca tggcgttgcc cgtgacggca ctgttgctcc
      721 cactcgcgct gctgctgcac gctgcaaggc ctgaacaaaa acttatcagc gaagaggatc
      781 tggatatcca gatgacccag tccccgagct ccctgtccgc ctctgtgggc gatagggtca
      841 ccatcacctg ccgtgccagt cagtccgtgt ccagcgctgt agcctggtat caacagaaac
      901 caggaaaagc tccgaagctt ctgatttact cggcatccga tctctactct ggagtccctt
      961 ctcgcttctc tggtagccgt tccgggacgg atttcactct gaccatcagc agtctgcagc
     1021 cggaagactt cgcaacttat tactgtcagc aatcttctta ttctctgatc acgttcggac
     1081 agggtaccaa ggtggagatc aaaggtacta ctgccgctag tggtagtagt ggtggcagta
     1141 gcagtggtgc cgaggttcag ctggtggagt ctggcggtgg cctggtgcag ccagggggct
     1201 cactccgttt gtcctgtgca gcttctggct tcgatatcag tagttattcg atgcactggg
     1261 tgcgtcaggc cccgggtaag ggcctggaat gggttgcacg tatttactct tctggaggca
     1321 ctactgctta tgccgatagc gtcaagggcc gtttcactat aagcgcagac acatccaaaa
     1381 acacagccta cctacaaatg aacagcttaa gagctgagga cactgccgtc tattattgtg
     1441 ctcgcggtca ttggttcttc cattactctt actacttcta cttcccgcgt tactacggtt
     1501 tggactactg gggtcaagga accctggtca ccgtctcctc gatcctggac tacagcttca
     1561 caggtggcgc tgggcgcgac attcccccac cgcagattga ggaggcctgt gagctgcctg
     1621 agtgccaggt ggatgcaggc aataaggtct gcaacctgca gtgtaataat cacgcatgtg
     1681 gctgggatgg tggcgactgc tccctcaact tcaatgaccc ctggaagaac tgcacgcagt
     1741 ctctacagtg ctggaagtat tttagcgacg gccactgtga cagccagtgc aactcggccg
     1801 gctgcctctt tgatggcttc gactgccagc tcaccgaggg acagtgcaac cccctgtatg
     1861 accagtactg caaggaccac ttcagtgatg gccactgcga ccagggctgt aacagtgccg
     1921 aatgtgagtg ggatggccta gactgtgctg agcatgtacc cgagcggctg gcagccggca
     1981 ccctggtgct ggtggtgctg cttccacccg accagctacg gaacaactcc ttccactttc
     2041 tgcgggagct cagccacgtg ctgcacacca acgtggtctt caagcgtgat gcgcaaggcc
     2101 agcagatgat cttcccgtac tatggccacg aggaagagct gcgcaagcac ccaatcaagc
     2161 gctctacagt gggttgggcc acctcttcac tgcttcctgg taccagtggt gggcgccagc
     2221 gcagggagct ggaccccatg gacatccgtg gctccattgt ctacctggag atcgacaacc
     2281 ggcaatgtgt gcagtcatcc tcgcagtgct tccagagtgc caccgatgtg gctgccttcc
     2341 taggtgctct tgcgtcactt ggcagcctca atattcctta caagattgag gccgtgaaga
     2401 gtgagccggt ggagcctccg ctgccctcgc agctgcacct catgtacgtg gcagcggccg
     2461 ccttcgtgct cctgttcttt gtgggctgtg gggtgctgct gtcccgcaag cgccggcgga
     2521 tgaagctgct gagcagcatc gagcaggcct gtgacatctg ccggctgaag aaactgaagt
     2581 gcagcaaaga aaagcccaag tgcgccaagt gcctgaagaa caactgggag tgccggtaca
     2641 gccccaagac caagagaagc cccctgacca gagcccacct gaccgaggtg gaaagccggc
     2701 tggaaagact ggaacagctg tttctgctga tcttcccacg cgaggacctg gacatgatcc
     2761 tgaagatgga cagcctgcag gacatcaagg ccctgctgac cggcctgttc gtgcaggaca
     2821 acgtgaacaa ggacgccgtg accgacagac tggccagcgt ggaaaccgac atgcccctga
     2881 ccctgcggca gcacagaatc agcgccacca gcagcagcga ggaaagcagc aacaagggcc
     2941 agcggcagct gacagtgtct gctgctgcag gcggaagcgg aggctctggc ggatctgatg
     3001 ccctggacga cttcgacctg gatatgctgg gcagcgacgc cctggatgat tttgatctgg
     3061 acatgctggg atctgacgct ctggacgatt tcgatctcga catgttggga tcagatgcac
     3121 tggatgactt tgacctggac atgctcggat catgagatcc ttgacttgcg gccgcaactc
     3181 ccacctgcaa catgcgtgac tgactgaggc cgcgactcta gagtcgacct gcaggcatgc
     3241 aagcttgata tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
     3301 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     3361 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     3421 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     3481 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     3541 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     3601 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     3661 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     3721 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     3781 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     3841 gccgcctccc cgcatcgata ccgtcgacct cgagggaatt aattcgagct cggtaccttt
     3901 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     3961 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     4021 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     4081 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     4141 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat
     4201 ctagaattaa ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat gatacataag
     4261 gttatgtatt aattgtagcc gcgttctaac gacaatatgt acaagcctaa ttgtgtagca
     4321 tctggcttac tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg
     4381 gttggacgaa ccttctgagt ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa
     4441 ccaatcagca gggtcatcgc tagccagatc ctctacgccg gacgcatcgt ggccggcatc
     4501 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
     4561 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
     4621 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg
     4681 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
     4741 cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     4801 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     4861 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     4921 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     4981 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     5041 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     5101 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     5161 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     5221 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     5281 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     5341 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     5401 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     5461 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     5521 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     5581 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     5641 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     5701 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     5761 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     5821 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     5881 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     5941 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     6001 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     6061 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     6121 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     6181 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     6241 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     6301 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     6361 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     6421 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     6481 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     6541 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     6601 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     6661 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     6721 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     6781 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     6841 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     6901 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctcac
     6961 cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag
     7021 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     7081 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     7141 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     7201 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     7261 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     7321 tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt ttgagaatac cactttatcc
     7381 cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag
     7441 tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt
     7501 agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt
     7561 gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat
     7621 tattgccgta agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt
     7681 cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa
     7741 gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat
     7801 accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc
     7861 ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat
     7921 acctggattg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc
     7981 taatcttttc aacgcctggc actgccgggc gttgttcttt ttaacttcag gcgggttaca
     8041 atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc tgagcgaaac
     8101 cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttaatca
     8161 cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa accatccctc actggtatcg
     8221 catgattaac cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt
     8281 ccaggcacgt attgtgatga gcgatgccga acgtaccgac gatgatttat acgatacggt
     8341 gattggctac cgtggcggca actggattta tgagtgggcc ccggatcttt gtgaaggaac
     8401 cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg
     8461 taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat
     8521 tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg
     8581 aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc
     8641 aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag
     8701 aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta
     8761 tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg
     8821 taacctttat aagtaggcat aacagttata atcataacat actgtttttt cttactccac
     8881 acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt
     8941 taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata
     9001 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     9061 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     9121 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     9181 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg
     9241 ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc
     9301 aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa
     9361 agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc
     9421 ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
     9481 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta
     9541 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag
     9601 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg
     9661 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt
     9721 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag
     9781 gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc
     9841 tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     9901 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     9961 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
    10021 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
    10081 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
    10141 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
    10201 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
    10261 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
    10321 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
    10381 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
    10441 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
    10501 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc
    10561 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
    10621 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
    10681 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
    10741 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
    10801 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
    10861 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
    10921 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
    10981 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
    11041 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
    11101 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
    11161 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
    11221 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
    11281 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
    11341 gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga
    11401 ttagtgaacg gatctcgacg gtatcgccaa atggcagtat tcatccacaa ttttaaaaga
    11461 aaagggggga ttggggggta cagtgcaggg g
//
