LOCUS       sequence-49168-e         472 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 472)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: BPTF
FEATURES             Location/Qualifiers
     source          1..472
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tgcagtgtca gagcaccgaa gatgccatga ccgtgctgac cccgctgacc
      121 gaaaaagatt atgaaggtct gaaacgtgtt ctgcgcagcc tgcaggcgca taaaatggcg
      181 tggccgtttc tggaaccggt tgatccgaat gatgcgccgg attattatgg cgtgattaaa
      241 gaaccgatgg atctggcgac catggaagaa cgtgttcagc gtcgctatta tgaaaaactg
      301 accgaatttg tggcggatat gaccaaaatt tttgataact gccgttatta taatccgagc
      361 gatagcccgt tttatcagtg cgcggaagtt ctggaaagct tttttgtgca gaaactgaaa
      421 ggctttaaag ccagccgcag ccattgacag taaaggtgga tacggatccg aa
//
