LOCUS       sequence-491698-       11037 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of the cpCys203 FRET-based cysteine sensor in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11037)
  AUTHORS   Abrams RPM, Donahue RG, Ma J, Mao Y, Diolaiti ME, Ashworth A
  TITLE     An engineered cysteine sensor optimized for high-throughput 
            screening identifies regulators of intracellular thiol levels.
  JOURNAL   Cell Chem Biol. 2025 Nov 10:S2451-9456(25)00343-5. doi: 
            10.1016/j.chembiol.2025.10.006.
  PUBMED    41218609
REFERENCE   2  (bases 1 to 11037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Chem 
            Biol. 2025 Nov 10:S2451-9456(25)00343-5. doi: 
            10.1016/j.chembiol.2025.10.006."
COMMENT     Sequence Label: pLVX_cpCys203
FEATURES             Location/Qualifiers
     source          1..11037
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2337..3519
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2568..3510
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3467..3487
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3544..3553
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3856..3875)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             5370..5381
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     misc_feature    join(5787..6361,6362..6364,6365..6373)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5954..5971)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6181..6200
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6393..6992
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6393..6412)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6889..6909
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7006..7594
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7059..7079)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7477..7488)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7801..8434
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8562..8578)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8562..8578)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8575..8597)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8586..8602
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8610..8616,8617..8634,8635..8640))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8655..8676
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8793..8810)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8964..9552)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9044..9063)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9723..10514,10515..10583))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10346..10365
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10584..10688)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10736..10870
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10773..10792)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10827..10846
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg
     2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
     2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
     2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatctatttc cggtgaattc
     3541 gccgccacca tggtttccaa gggcgaagaa ctgttcaccg gcgtggtgcc cattctggtg
     3601 gaactggacg gggatgtgaa cggccacaag tttagcgtta gcggcgaagg cgaaggggat
     3661 gccacatacg gaaagctgac cctgaagttc atctgcacca ccggcaagct gcctgtgcct
     3721 tggcctacac tggtcaccac actgacatgg ggcgtgcagt gcttcagcag ataccccgac
     3781 catatgaagc agcacgactt cttcaagagc gccatgcctg agggctacgt gcaagagcgg
     3841 accatcttct ttaaggacga cggcaactac aagaccaggg ccgaagtgaa gttcgagggc
     3901 gacaccctgg tcaaccggat cgagctgaag ggcatcgact tcaaagagga cggcaacatc
     3961 ctgggccaca agctcgagta caactacatc agccacaacg tgtacatcac cgccgacaag
     4021 cagaagaacg gcatcaaggc caacttcaag atccggcaca acatcgagga cggctctgtg
     4081 cagctggccg atcactacca gcagaacaca cccatcggag atggccctgt gctgctgccc
     4141 gataaccact acctgagcac acagagcgcc ctgagcaagg accccaacga gaagagggat
     4201 cacatggtgc tgctggaatt cgtgaccgcc gctggcatca cactcggcat ggacgaactg
     4261 tacaaagtaa tagcgccagc cgtcaaaaag ggggataagg aactgaagga gtttatcgac
     4321 aatctcatca caaaattggg ggaggagcaa ttctttcata aggcttacga tgagaccctc
     4381 aagagtcatt tcggggatga tgtgaaggcc gacgacgtag taattgaagg tgggaagatc
     4441 ggtgggtccg gtggcagtgg gggctctggc ggtagcggtg ggaatagtga tagtggtgcg
     4501 agtaactctc ttgagcgaat aaagcaagat ggagtggttc gaattggtgt ttttggcgat
     4561 aagccgccgt ttggatacgt tgacgagaaa ggcgtaaatc aaggatacga catcgttctg
     4621 gcgaagagaa tagcgaaaga acttctgggg gacgagaata aggttcagtt tgtcttggtg
     4681 gaggcagcca accgggtcga gttcctcaag tcaaacaaag tggatataat acttgccaat
     4741 tttactcaga cacctgaaag agcagagcag gtagattttt gtcttcctta catgaaggtc
     4801 gctctcggag tggccgtacc tcaagactct aacatatcta gtattgaaga tttgaaggat
     4861 aagactctgc tgctcaacaa aggcaccaca gcggacgcat atttcacaaa ggaataccct
     4921 gacatcaaga ctcttaagta cgatcagaac acggaaacat ttgccgcgct catcgatcag
     4981 aggggagatg cgctttccca cgataatacc ctcttgttcg cgtgggtgaa ggaacaccct
     5041 gaatttaaaa tggcaatcat ggtgtctaaa ggcgaggaac tctttacagg cgtggtccct
     5101 atcctcgtcg agctggatgg cgacgtgaac ggacataagt tctccgtgtc tggcgaaggc
     5161 gagggcgacg ctacctatgg caagctgaca ctcaagctga tctgtacaac agggaaactg
     5221 cccgtgccat ggccaactct cgtgacaaca ctcggctacg gcctgcagtg tttcgccaga
     5281 tatcctgatc atatgaagca acatgatttc tttaagtccg ctatgcccga gggatatgtc
     5341 caagagagaa cgatcttttt caaggatgat gggaattaca aaacccgcgc tgaagtcaag
     5401 tttgaaggcg atacactcgt gaaccgcatt gagctgaaag ggattgattt caaagaggat
     5461 gggaatattc tcggacacaa acttgagtac aattataact cccacaatgt ctatatcacg
     5521 gctgataagc agaaaaatgg gatcaaagct aatttcaaga tcagacataa tattgaggat
     5581 ggcggcgtcc agctggctga ccattatcag caaaacaccc ctatcggcga cggacccgtt
     5641 ctgctgcctg acaatcacta tctgtcctac cagtccgctc tgtccaagga tcctaatgag
     5701 aaacgcgacc acatggtcct tctcgagttt gtgacagccg ccggaattac cctcggaatg
     5761 gatgagcttt acaaatgagg atcccgcccc tctccctccc ccccccctaa cgttactggc
     5821 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     5881 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     5941 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     6001 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     6061 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     6121 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     6181 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     6241 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
     6301 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
     6361 gcttgccaca acccacaagg agacgacctt ccatgaccga gtacaagccc acggtgcgcc
     6421 tcgccacccg cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg ttcgccgact
     6481 accccgccac gcgccacacc gtcgacccgg accgccacat cgagcgggtc accgagctgc
     6541 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     6601 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     6661 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     6721 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     6781 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     6841 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     6901 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     6961 cctggtgcat gacccgcaag cccggtgcct agacgcgtct ggaacaatca acctctggat
     7021 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     7081 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     7141 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     7201 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     7261 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     7321 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     7381 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     7441 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     7501 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     7561 acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc
     7621 tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
     7681 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa
     7741 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aagaggggac
     7801 tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca
     7861 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
     7921 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
     7981 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
     8041 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
     8101 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
     8161 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
     8221 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
     8281 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     8341 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     8401 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     8461 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggccttg acattgctag
     8521 cgtttaccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     8581 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     8641 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     8701 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     8761 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     8821 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     8881 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     8941 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     9001 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     9061 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     9121 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     9181 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     9241 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     9301 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     9361 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     9421 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     9481 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     9541 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     9601 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     9661 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     9721 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     9781 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     9841 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     9901 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     9961 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
    10021 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
    10081 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
    10141 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
    10201 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
    10261 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
    10321 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
    10381 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    10441 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    10501 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    10561 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    10621 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    10681 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcaactt
    10741 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
    10801 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
    10861 tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata
    10921 ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga
    10981 attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagt
//
