LOCUS       sequence-49171-e        1522 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1522)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: C14orf169
FEATURES             Location/Qualifiers
     source          1..1522
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1451..1471
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1472..1489
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1490..1513
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cttaagaagg agatatacta tggcgtcggg gccgcaggtg gataacacgg gtggggagcc
       61 ggcctgggac tccccgctgc ggcgcgtctt ggccgagctg aaccgcatcc ccagcagccg
      121 gcggcgagcg gcccgcctct ttgagtggct catcgcgccc atgccgccag atcacttcta
      181 ccggcgccta tgggagcgcg aggcggtgct ggtgcggcgg caggaccaca cctactacca
      241 gggacttttc tctaccgctg acctggattc gatgctgcgc aacgaggagg tgcagttcgg
      301 ccagcatttg gacgccgctc gctacatcaa cggacgacgc gagaccctga acccacccgg
      361 ccgcgcgctg cccgccgccg cgtggtccct gtaccaggcc ggctgctccc tgcgtctcct
      421 ctgtccgcag gctttctcta ctactgtgtg gcagtttttg gctgtgcttc aagagcagtt
      481 tggaagcatg gcaggctcca acgtttacct cacgccccct aactcgcagg gctttgcccc
      541 ccactacgac gacatcgagg ccttcgtgct gcagctggaa ggtaggaaac tctggcgtgt
      601 ataccgaccc cgagccccaa ccgaggaact ggctctgaca tccagcccca acttcagtca
      661 ggacgacctc ggtgagccgg tgctgcagac cgtgctggaa cctggagatt tgctgtattt
      721 tcctcggggc ttcattcacc aagctgaatg ccaggatgga gtccactctc tgcacctcac
      781 cttgtccacg taccagcgca atacctgggg tgacttctta gaggccatac tgcctctggc
      841 agtgcaggct gcaatggaag aaaatgtgga gtttcggagg ggtctgcccc gagacttcat
      901 ggattacatg ggggcccagc attcagattc taaggatccg cgaagaaccg ctttcatgga
      961 gaaggtgcgg gtcttggttg cccgcctggg acactttgct cctgttgatg ctgtggccga
     1021 ccagcgagcc aaagacttca ttcacgattc tctgccccct gttttgactg atagggagag
     1081 ggcactaagt gtttacgggc ttccaattcg ctgggaggct ggagaacctg taaacgtggg
     1141 ggcccagttg acaacagaaa cagaagtcca tatgcttcag gatgggatag ctcggctggt
     1201 gggtgagggg ggccatttgt ttctctatta cacagtggaa aactcccgtg tgtatcatct
     1261 ggaagaaccc aagtgcttgg aaatataccc ccagcaagct gatgccatgg aactgttgct
     1321 tggttcttat ccagagtttg tgagagtggg ggacctgccc tgtgacagtg tggaggacca
     1381 gctgtccttg gcaaccacgt tgtatgataa ggggctgctg ctcactaaga tgcctctagc
     1441 cctaaatgca gagaacctct acttccaatc gcaccatcat caccaccatg attacaagga
     1501 tgacgacgat aagtgaggat cc
//
