LOCUS       sequence-491750-       10678 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GAL4/UAS expression of GFP and constitutive expression of BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10678)
  AUTHORS   Simic MS, Watchmaker PB, Gupta S, Wang Y, Sagan SA, Duecker J, 
            Shepherd C, Diebold D, Pineo-Cavanaugh P, Haegelin J, Zhu R, Ng B, 
            Yu W, Tonai Y, Cardarelli L, Reddy NR, Sidhu SS, Troyanskaya O, 
            Hauser SL, Wilson MR, Zamvil SS, Okada H, Lim WA
  TITLE     Programming tissue-sensing T cells that deliver therapies to the 
            brain.
  JOURNAL   Science. 2024 Dec 6;386(6726):eadl4237. doi: 
            10.1126/science.adl4237. Epub 2024 Dec 6.
  PUBMED    39636984
REFERENCE   2  (bases 1 to 10678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2024-12-6"; volume: "386(6726):eadl4237. doi"; pages: " 
            10.1126/science.adl4237. Epub 2024 Dec 6"
COMMENT     Sequence Label: pHR-Gal4UAS-GFP-PGK-BFP
FEATURES             Location/Qualifiers
     source          1..10678
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             49..282
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(307..325)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(308..325)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(615..634)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(848..867)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     967..989
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1027..1045)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1113..1217
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1218..1286,1287..2078)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1436..1455)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2249..2837
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2738..2757
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2991..3008
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3078..3098)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3083..3412
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3263..3398
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4546..4611
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4741..4761
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5186..5320
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5223..5242)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5277..5296
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5488..6121
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6168..6293
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6790..7023
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7208..7252
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7537..7654
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     7880..7904
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(7985..7987,7988..7990,7991..8704)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8030..8051)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8291..8310)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8638..8659
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        8710..9197
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8745..8768)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9130..9149
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             9218..9919
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    10029..10617
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10082..10102)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10500..10511)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10620..10636)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10621..10637)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
       61 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
      121 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
      181 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
      241 gatccctcag acccttttag tcagtgtgga aaatctctag cagcatctag aattaattcc
      301 gtgtattcta tagtgtcacc taaatcgtat gtgtatgata cataaggtta tgtattaatt
      361 gtagccgcgt tctaacgaca atatgtacaa gcctaattgt gtagcatctg gcttactgaa
      421 gcagacccta tcatctctct cgtaaactgc cgtcagagtc ggtttggttg gacgaacctt
      481 ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc agcgaaccaa tcagcagggt
      541 catcgctagc cagatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg
      601 tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt
      661 cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact
      721 gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa
      781 cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gaatggtgca
      841 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
      901 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
      961 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     1021 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     1081 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     1141 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     1201 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     1261 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     1321 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     1381 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     1441 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     1501 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     1561 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     1621 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     1681 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     1741 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     1801 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     1861 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     1921 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     1981 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     2041 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     2101 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     2161 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     2221 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     2281 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     2341 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     2401 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     2461 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     2521 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     2581 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     2641 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     2701 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     2761 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     2821 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     2881 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     2941 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     3001 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     3061 ttcattaatg cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     3121 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     3181 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     3241 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     3301 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     3361 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttgg
     3421 acacaagaca ggcttgcgag atatgtttga gaataccact ttatcccgcg tcagggagag
     3481 gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg ccagatctct
     3541 ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat aaacaggctg
     3601 ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag atccatgcac
     3661 gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt gccgtaagcc
     3721 gtggcggtct gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac
     3781 cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc
     3841 agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc
     3901 ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc
     3961 tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct ggattgaaca
     4021 gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg
     4081 cctggcactg ccgggcgttg ttctttttaa cttcaggcgg gttacaatag tttccagtaa
     4141 gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc
     4201 gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc
     4261 ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc
     4321 tgatgtggat ctggcgcggc attgacccac gcgaaatcct cgacgtccag gcacgtattg
     4381 tgatgagcga tgccgaacgt accgacgatg atttatacga tacggtgatt ggctaccgtg
     4441 gcggcaactg gatttatgag tgggccccgg atctttgtga aggaacctta cttctgtggt
     4501 gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt
     4561 ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc
     4621 tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc
     4681 tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct
     4741 ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt
     4801 ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag
     4861 gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac ctttataagt
     4921 aggcataaca gttataatca taacatactg ttttttctta ctccacacag gcatagagtg
     4981 tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg
     5041 gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac
     5101 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     5161 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     5221 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     5281 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
     5341 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt
     5401 tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg
     5461 ttaaatatgt actacaaact tagtagttgg aagggctaat tcactcccaa agaagacaag
     5521 atatccttga tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca
     5581 caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag
     5641 ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg
     5701 tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc
     5761 gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat
     5821 atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg
     5881 ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact
     5941 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     6001 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     6061 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     6121 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     6181 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     6241 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     6301 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     6361 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     6421 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     6481 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     6541 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     6601 aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca gacctggagg
     6661 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     6721 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     6781 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     6841 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     6901 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     6961 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     7021 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     7081 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     7141 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     7201 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     7261 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     7321 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     7381 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
     7441 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
     7501 tcgacggtat cgccaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     7561 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     7621 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     7681 tccagtttgg atcgataagc ttgatatcga attcggagca ctgtcctccg aacgtcggag
     7741 cactgtcctc cgaacgtcgg agcactgtcc tccgaacgtc ggagcactgt cctccgaacg
     7801 gagcatgtcc tccgaacgtc ggagcactgt cctccgaacg actagttagg cgtgtacggt
     7861 gggaggccta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc
     7921 cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctctcg acattcgttg
     7981 gatcatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     8041 ggacggcgac gtaaacggcc acaagttcag cgtgtctggc gagggcgagg gcgatgccac
     8101 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     8161 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     8221 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     8281 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     8341 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     8401 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     8461 gaacggcatc aaggcgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     8521 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     8581 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     8641 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     8701 gtaagatccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag
     8761 ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca
     8821 ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc
     8881 ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg
     8941 aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg
     9001 taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc
     9061 tgggaagggg tgggtccggg ggcgggctca ggggcggggc gggcgcccga aggtcctccg
     9121 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
     9181 catctccggg cctttcgacc tgcagcccaa gcttaccatg agcgagctga ttaaggagaa
     9241 catgcacatg aagctgtaca tggagggcac cgtggacaac catcacttca agtgcacatc
     9301 cgagggcgaa ggcaagccct acgagggcac ccagaccatg agaatcaagg tggtcgaggg
     9361 cggccctctc cccttcgcct tcgacatcct ggctactagc ttcctctacg gcagcaagac
     9421 cttcatcaac cacacccagg gcatccccga cttcttcaag cagtccttcc ctgagggctt
     9481 cacatgggag agagtcacca catacgaaga cgggggcgtg ctgaccgcta cccaggacac
     9541 cagcctccag gacggctgcc tcatctacaa cgtcaagatc agaggggtga acttcacatc
     9601 caacggccct gtgatgcaga agaaaacact cggctgggag gccttcaccg agacgctgta
     9661 ccccgctgac ggcggcctgg aaggcagaaa cgacatggcc ctgaagctcg tgggcgggag
     9721 ccatctgatc gcaaacatca agaccacata tagatccaag aaacccgcta agaacctcaa
     9781 gatgcctggc gtctactatg tggactacag actggaaaga atcaaggagg ccaacaacga
     9841 gacctacgtc gagcagcacg aggtggcagt ggccagatac tgcgacctcc ctagcaaact
     9901 ggggcacaag cttaattaag atccttgact tgcggccgca actcccacct gcaacatgcg
     9961 tgactgactg aggccgcgac tctagagtcg acctgcaggc atgcaagctt gatatcaagc
    10021 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
    10081 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
    10141 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
    10201 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
    10261 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
    10321 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
    10381 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
    10441 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
    10501 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
    10561 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
    10621 gataccgtcg acctcgaggg aattaattcg agctcggtac ctttaagacc aatgactt
//
