LOCUS       sequence-49176-e         970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 970)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: AURKB
FEATURES             Location/Qualifiers
     source          1..970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             643..654
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tgcagaaggt gatggagaat agcagtggga cacccgacat cttaacgcgg
      121 cacttcacaa ttgatgactt tgagattggg cgtcctctgg gcaaaggcaa gtttggaaac
      181 gtgtacttgg ctcgggagaa gaaaagccat ttcatcgtgg cgctcaaggt cctcttcaag
      241 tcccagatag agaaggaggg cgtggagcat cagctgcgca gagagatcga aatccaggcc
      301 cacctgcacc atcccaacat cctgcgtctc tacaactatt tttatgaccg gaggaggatc
      361 tacttgattc tagagtatgc cccccgcggg gagctctaca aggagctgca gaagagctgc
      421 acatttgacg agcagcgaac agccacgatc atggaggagt tggcagatgc tctaatgtac
      481 tgccatggga agaaggtgat tcacagagac ataaagccag aaaatctgct cttagggctc
      541 aagggagagc tgaagattgc tgacttcggc tggtctgtgc atgcgccctc cctgaggagg
      601 aagacaatgt gtggcaccct ggactacctg cccccagaga tgattgaggg gcgcatgcac
      661 aatgagaagg tggatctgtg gtgcattgga gtgctttgct atgagctgct ggtggggaac
      721 ccaccctttg agagtgcatc acacaacgag acctatcgcc gcatcgtcaa ggtggaccta
      781 aagttccccg cttctgtgcc cacgggagcc caggacctca tctccaaact gctcaggcat
      841 aacccctcgg aacggctgcc cctggcccag gtctcagccc acccttgggt ccgggccaac
      901 tctcggaggg tgctgcctcc ctctgccctt caatctgtcg cctgacagta aaggtggata
      961 cggatccgaa
//
