LOCUS       sequence-49177-e        1109 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1109)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GUCY1A3
FEATURES             Location/Qualifiers
     source          1..1109
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             34..360
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
                     IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
                     DANLA"
     CDS             367..387
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtatgagcg ataaaattat tcacctgact
       61 gacgacagtt ttgacacgga tgtactcaaa gcggacgggg cgatcctcgt cgatttctgg
      121 gcagagtggt gcggtccgtg caaaatgatc gccccgattc tggatgaaat cgctgacgaa
      181 tatcagggca aactgaccgt tgcaaaactg aacatcgatc aaaaccctgg cactgcgccg
      241 aaatatggca tccgtggtat cccgactctg ctgctgttca aaaacggtga agtggcggca
      301 accaaagtgg gcgcactgtc taaaggtcag ttgaaagagt tcctcgacgc taacctggcc
      361 ggtaccgaga acttgtactt ccaatccatg caaggtcagg tcgttcaagc taagaaattc
      421 agcaatgtga cgatgctgtt tagtgatatc gtagggttca ccgccatttg ctcacagtgt
      481 tccccgctgc aggtcatcac tatgctgaac gcactgtaca cacgctttga ccaacagtgc
      541 ggcgaactgg atgtttataa agtggagaca attggtgacg cgtactgtgt agctggcggt
      601 ctgcataagg aaagcgatac ccacgccgtc caaatcgcac tgatggcgct gaaaatgatg
      661 gaactgtcgg atgaggttat gtctccgcat ggggaaccga ttaaaatgcg tattggcctg
      721 catagtggtt cagtgttcgc cggggtagtc ggcgttaaga tgccgcgcta ttgcctgttt
      781 ggtaataacg tgactctggc aaataaattt gaaagctgtt ccgtaccgcg taaaatcaac
      841 gtatcgccga cgacctaccg cctgctgaag gactgcccgg gcttcgtttt tacaccgcgt
      901 tctcgcgagg aactgccgcc gaatttcccg agtgagattc cgggtatctg tcactttctg
      961 gatgcgtatc agcaggggac caactcaaaa ccgtgcttcc aaaaaaagga cgtggaagat
     1021 ggcaatgcta actttctggg taaagccagc ggcattgatt gacagtaaag gtggatacgg
     1081 atccgaattc gagctccgtc gacaagctt
//
