LOCUS       sequence-491773-       11452 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a synNotch receptor binding to BCAN.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11452)
  AUTHORS   Simic MS, Watchmaker PB, Gupta S, Wang Y, Sagan SA, Duecker J, 
            Shepherd C, Diebold D, Pineo-Cavanaugh P, Haegelin J, Zhu R, Ng B, 
            Yu W, Tonai Y, Cardarelli L, Reddy NR, Sidhu SS, Troyanskaya O, 
            Hauser SL, Wilson MR, Zamvil SS, Okada H, Lim WA
  TITLE     Programming tissue-sensing T cells that deliver therapies to the 
            brain.
  JOURNAL   Science. 2024 Dec 6;386(6726):eadl4237. doi: 
            10.1126/science.adl4237. Epub 2024 Dec 6.
  PUBMED    39636984
REFERENCE   2  (bases 1 to 11452)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2024-12-6"; volume: "386(6726):eadl4237. doi"; pages: " 
            10.1126/science.adl4237. Epub 2024 Dec 6"
COMMENT     Sequence Label: pHR_PGK_ BCAN 13134 VLVH synNotch
FEATURES             Location/Qualifiers
     source          1..11452
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        137..636
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(172..195)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     569..588
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      684..693
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             753..782
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2481..2921
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             2958..3107
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     misc_feature    3226..3814
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3279..3299)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3697..3708)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3817..3833)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3818..3834)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3924..4157
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4182..4200)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4183..4200)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4490..4509)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4723..4742)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4842..4864
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4902..4920)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4988..5092
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5093..5161,5162..5953)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5311..5330)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6124..6712
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6613..6632
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6866..6883
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6953..6973)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6958..7287
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7138..7273
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8421..8486
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8616..8636
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9061..9195
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9098..9117)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9152..9171
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9363..9996
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10043..10168
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10665..10898
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11083..11127
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    join(11412..11452,1..77)
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
       61 caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt tggatcgata
      121 agcttgatat cgaattgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct
      181 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc
      241 acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct
      301 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga
      361 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg
      421 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct
      481 cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg
      541 ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc
      601 gcgctgttct cctcttcctc atctccgggc ctttcgaatt ctcacgcgtc aagtggagca
      661 aggcaggtgg acagtggatc gccgccacca tggcgttgcc cgtgacggca ctgttgctcc
      721 cactcgcgct gctgctgcac gctgcaaggc ctgaacaaaa acttatcagc gaagaggatc
      781 tggatatcca gatgacccag tccccgagct ccctgtccgc ctctgtgggc gatagggtca
      841 ccatcacctg ccgtgccagt cagtccgtgt ccagcgctgt agcctggtat caacagaaac
      901 caggaaaagc tccgaagctt ctgatttact cggcatccga tctctactct ggagtccctt
      961 ctcgcttctc tggtagccgt tccgggacgg atttcactct gaccatcagc agtctgcagc
     1021 cggaagactt cgcaacttat tactgtcagc aatacggtga cccgatcacg ttcggacagg
     1081 gtaccaaggt ggagatcaaa ggtactactg ccgctagtgg tagtagtggt ggcagtagca
     1141 gtggtgccga ggttcagctg gtggagtctg gcggtggcct ggtgcagcca gggggctcac
     1201 tccgtttgtc ctgtgcagct tctggcttcg atctcagtac ttattggatg cactgggtgc
     1261 gtcaggcccc gggtaagggc ctggaatggg ttgcaagtat ttacgcttct ggaggcgcta
     1321 ctgattatgc cgatagcgtc aagggccgtt tcactataag cgcagacaca tccaaaaaca
     1381 cagcctacct acaaatgaac agcttaagag ctgaggacac tgccgtctat tattgtgctc
     1441 gctactacca ttacccggct atggactact ggggtcaagg aaccctggtc accgtctcct
     1501 cgatcctgga ctacagcttc acaggtggcg ctgggcgcga cattccccca ccgcagattg
     1561 aggaggcctg tgagctgcct gagtgccagg tggatgcagg caataaggtc tgcaacctgc
     1621 agtgtaataa tcacgcatgt ggctgggatg gtggcgactg ctccctcaac ttcaatgacc
     1681 cctggaagaa ctgcacgcag tctctacagt gctggaagta ttttagcgac ggccactgtg
     1741 acagccagtg caactcggcc ggctgcctct ttgatggctt cgactgccag ctcaccgagg
     1801 gacagtgcaa ccccctgtat gaccagtact gcaaggacca cttcagtgat ggccactgcg
     1861 accagggctg taacagtgcc gaatgtgagt gggatggcct agactgtgct gagcatgtac
     1921 ccgagcggct ggcagccggc accctggtgc tggtggtgct gcttccaccc gaccagctac
     1981 ggaacaactc cttccacttt ctgcgggagc tcagccacgt gctgcacacc aacgtggtct
     2041 tcaagcgtga tgcgcaaggc cagcagatga tcttcccgta ctatggccac gaggaagagc
     2101 tgcgcaagca cccaatcaag cgctctacag tgggttgggc cacctcttca ctgcttcctg
     2161 gtaccagtgg tgggcgccag cgcagggagc tggaccccat ggacatccgt ggctccattg
     2221 tctacctgga gatcgacaac cggcaatgtg tgcagtcatc ctcgcagtgc ttccagagtg
     2281 ccaccgatgt ggctgccttc ctaggtgctc ttgcgtcact tggcagcctc aatattcctt
     2341 acaagattga ggccgtgaag agtgagccgg tggagcctcc gctgccctcg cagctgcacc
     2401 tcatgtacgt ggcagcggcc gccttcgtgc tcctgttctt tgtgggctgt ggggtgctgc
     2461 tgtcccgcaa gcgccggcgg atgaagctgc tgagcagcat cgagcaggcc tgtgacatct
     2521 gccggctgaa gaaactgaag tgcagcaaag aaaagcccaa gtgcgccaag tgcctgaaga
     2581 acaactggga gtgccggtac agccccaaga ccaagagaag ccccctgacc agagcccacc
     2641 tgaccgaggt ggaaagccgg ctggaaagac tggaacagct gtttctgctg atcttcccac
     2701 gcgaggacct ggacatgatc ctgaagatgg acagcctgca ggacatcaag gccctgctga
     2761 ccggcctgtt cgtgcaggac aacgtgaaca aggacgccgt gaccgacaga ctggccagcg
     2821 tggaaaccga catgcccctg accctgcggc agcacagaat cagcgccacc agcagcagcg
     2881 aggaaagcag caacaagggc cagcggcagc tgacagtgtc tgctgctgca ggcggaagcg
     2941 gaggctctgg cggatctgat gccctggacg acttcgacct ggatatgctg ggcagcgacg
     3001 ccctggatga ttttgatctg gacatgctgg gatctgacgc tctggacgat ttcgatctcg
     3061 acatgttggg atcagatgca ctggatgact ttgacctgga catgctcgga tcatgagatc
     3121 cttgacttgc ggccgcaact cccacctgca acatgcgtga ctgactgagg ccgcgactct
     3181 agagtcgacc tgcaggcatg caagcttgat atcaagctta tcgataatca acctctggat
     3241 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     3301 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     3361 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     3421 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     3481 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     3541 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     3601 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     3661 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     3721 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     3781 acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgagggaat
     3841 taattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     3901 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     3961 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     4021 ctaactaggg aacctactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     4081 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     4141 gtggaaaatc tctagcagca tctagaatta attccgtgta ttctatagtg tcacctaaat
     4201 cgtatgtgta tgatacataa ggttatgtat taattgtagc cgcgttctaa cgacaatatg
     4261 tacaagccta attgtgtagc atctggctta ctgaagcaga ccctatcatc tctctcgtaa
     4321 actgccgtca gagtcggttt ggttggacga accttctgag tttctggtaa cgccgtcccg
     4381 cacccggaaa tggtcagcga accaatcagc agggtcatcg ctagccagat cctctacgcc
     4441 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc
     4501 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc
     4561 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca
     4621 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg
     4681 caggagtcgc ataagggaga gcgtcgaatg gtgcactctc agtacaatct gctctgatgc
     4741 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     4801 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     4861 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     4921 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     4981 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     5041 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     5101 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     5161 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     5221 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     5281 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     5341 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     5401 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     5461 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     5521 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     5581 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     5641 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     5701 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     5761 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     5821 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     5881 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     5941 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     6001 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     6061 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     6121 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     6181 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     6241 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     6301 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     6361 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     6421 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     6481 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     6541 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     6601 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     6661 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     6721 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     6781 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     6841 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     6901 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct gtggaatgtg
     6961 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     7021 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     7081 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     7141 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     7201 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     7261 ttttttggag gcctaggctt ttgcaaaaag cttggacaca agacaggctt gcgagatatg
     7321 tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc
     7381 atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac ctattttccc
     7441 ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga gcgaaaaata
     7501 catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc
     7561 ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta
     7621 ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt gtatttccag ctacgatcac
     7681 gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc
     7741 gttattgatg acctggtgga taccggtggt actgcggttg cgattcgtga aatgtatcca
     7801 aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt tgatgactat
     7861 gttgttgata tcccgcaaga tacctggatt gaacagccgt gggatatggg cgtcgtattc
     7921 gtcccgccaa tctccggtcg ctaatctttt caacgcctgg cactgccggg cgttgttctt
     7981 tttaacttca ggcgggttac aatagtttcc agtaagtatt ctggaggctg catccatgac
     8041 acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg aaacctcgac
     8101 gctagtccgc cgctttaatc acggcgcaca accgcctgtg cagtcggccc ttgatggtaa
     8161 aaccatccct cactggtatc gcatgattaa ccgtctgatg tggatctggc gcggcattga
     8221 cccacgcgaa atcctcgacg tccaggcacg tattgtgatg agcgatgccg aacgtaccga
     8281 cgatgattta tacgatacgg tgattggcta ccgtggcggc aactggattt atgagtgggc
     8341 cccggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta
     8401 cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac
     8461 tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt
     8521 ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg
     8581 atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag
     8641 accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata
     8701 gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga
     8761 aaattatgga aaaatattct gtaaccttta taagtaggca taacagttat aatcataaca
     8821 tactgttttt tcttactcca cacaggcata gagtgtctgc tattaataac tatgctcaaa
     8881 aattgtgtac ctttagcttt ttaatttgta aaggggttaa taaggaatat ttgatgtata
     8941 gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt acttgcttta
     9001 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     9061 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     9121 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     9181 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc
     9241 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc
     9301 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta
     9361 gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca
     9421 cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc
     9481 actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc
     9541 caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
     9601 ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg
     9661 agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc
     9721 cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag
     9781 atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct
     9841 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     9901 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     9961 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
    10021 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
    10081 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
    10141 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
    10201 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
    10261 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
    10321 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
    10381 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
    10441 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
    10501 gcaagcggcc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
    10561 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
    10621 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
    10681 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
    10741 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
    10801 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
    10861 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
    10921 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
    10981 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
    11041 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
    11101 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
    11161 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
    11221 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
    11281 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
    11341 gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcca aatggcagta
    11401 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg gg
//
