LOCUS       sequence-491775-       11470 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a synNotch binding to myelin oligodendrocyte glycoprotein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11470)
  AUTHORS   Simic MS, Watchmaker PB, Gupta S, Wang Y, Sagan SA, Duecker J, 
            Shepherd C, Diebold D, Pineo-Cavanaugh P, Haegelin J, Zhu R, Ng B, 
            Yu W, Tonai Y, Cardarelli L, Reddy NR, Sidhu SS, Troyanskaya O, 
            Hauser SL, Wilson MR, Zamvil SS, Okada H, Lim WA
  TITLE     Programming tissue-sensing T cells that deliver therapies to the 
            brain.
  JOURNAL   Science. 2024 Dec 6;386(6726):eadl4237. doi: 
            10.1126/science.adl4237. Epub 2024 Dec 6.
  PUBMED    39636984
REFERENCE   2  (bases 1 to 11470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2024-12-6"; volume: "386(6726):eadl4237. doi"; pages: " 
            10.1126/science.adl4237. Epub 2024 Dec 6"
COMMENT     Sequence Label: pHR-SFFV- MOG-VHVL-M38-synNotch-Gal4VP64
FEATURES             Location/Qualifiers
     source          1..11470
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..17)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             107..340
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(365..383)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(366..383)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(673..692)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(906..925)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1025..1047
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1085..1103)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1171..1275
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1276..1344,1345..2136)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1494..1513)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2307..2895
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2796..2815
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3049..3066
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3136..3156)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3141..3470
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3321..3456
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4604..4669
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4799..4819
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5244..5378
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5281..5300)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5335..5354
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5546..6179
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6226..6351
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6848..7081
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7266..7310
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7595..7712
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7865..8272
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             8400..8429
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             10134..10574
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             10611..10760
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     misc_feature    10879..11467
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10932..10952)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11350..11361)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(join(11470,1..16))
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gataccgtcg acctcgaggg aattaattcg agctcggtac ctttaagacc aatgacttac
       61 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
      121 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
      181 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
      241 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
      301 tccctcagac ccttttagtc agtgtggaaa atctctagca gcatctagaa ttaattccgt
      361 gtattctata gtgtcaccta aatcgtatgt gtatgataca taaggttatg tattaattgt
      421 agccgcgttc taacgacaat atgtacaagc ctaattgtgt agcatctggc ttactgaagc
      481 agaccctatc atctctctcg taaactgccg tcagagtcgg tttggttgga cgaaccttct
      541 gagtttctgg taacgccgtc ccgcacccgg aaatggtcag cgaaccaatc agcagggtca
      601 tcgctagcca gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg
      661 cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg
      721 ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt
      781 tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc
      841 tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga atggtgcact
      901 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
      961 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     1021 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     1081 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     1141 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     1201 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     1261 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     1321 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     1381 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     1441 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     1501 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     1561 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     1621 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     1681 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     1741 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     1801 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     1861 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     1921 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     1981 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     2041 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     2101 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     2161 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     2221 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     2281 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     2341 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     2401 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     2461 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     2521 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     2581 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     2641 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     2701 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     2761 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     2821 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     2881 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     2941 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     3001 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     3061 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     3121 cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     3181 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     3241 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     3301 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     3361 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     3421 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttggac
     3481 acaagacagg cttgcgagat atgtttgaga ataccacttt atcccgcgtc agggagaggc
     3541 agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatctctat
     3601 aatctcgcgc aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg
     3661 acacttcaca tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt
     3721 aaactcgcaa gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt
     3781 ggcggtctgt accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg
     3841 tttgtatttc cagctacgat cacgacaacc agcgcgagct taaagtgctg aaacgcgcag
     3901 aaggcgatgg cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgcgg
     3961 ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg
     4021 gtcgtccgct ggttgatgac tatgttgttg atatcccgca agatacctgg attgaacagc
     4081 cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc
     4141 tggcactgcc gggcgttgtt ctttttaact tcaggcgggt tacaatagtt tccagtaagt
     4201 attctggagg ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc
     4261 tttaaacatc ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct
     4321 gtgcagtcgg cccttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg
     4381 atgtggatct ggcgcggcat tgacccacgc gaaatcctcg acgtccaggc acgtattgtg
     4441 atgagcgatg ccgaacgtac cgacgatgat ttatacgata cggtgattgg ctaccgtggc
     4501 ggcaactgga tttatgagtg ggccccggat ctttgtgaag gaaccttact tctgtggtgt
     4561 gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt
     4621 aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta
     4681 tggaactgat gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc
     4741 agaagaaatg ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc
     4801 aaaaaagaag agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt
     4861 gagtcatgct gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga
     4921 aaaagctgca ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag
     4981 gcataacagt tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc
     5041 tgctattaat aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt
     5101 taataaggaa tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat
     5161 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     5221 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     5281 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     5341 tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc
     5401 aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc
     5461 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
     5521 aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat
     5581 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca
     5641 ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     5701 gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg
     5761 agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc
     5821 ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat
     5881 cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg
     5941 actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg
     6001 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     6061 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     6121 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     6181 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     6241 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     6301 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     6361 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     6421 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     6481 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     6541 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     6601 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     6661 aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag
     6721 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     6781 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     6841 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     6901 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     6961 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     7021 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     7081 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     7141 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     7201 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     7261 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     7321 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     7381 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     7441 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
     7501 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatctc
     7561 gacggtatcg ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     7621 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     7681 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     7741 cagtttggat cgataagctt gatatcgaat tcctgcagcc ccgataaaat aaaagatttt
     7801 atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc
     7861 tgcagtaacg ccattttgca aggcatggaa aaataccaaa ccaagaatag agaagttcag
     7921 atcaagggcg ggtacatgaa aatagctaac gttgggccaa acaggatatc tgcggtgagc
     7981 agtttcggcc ccggcccggg gccaagaaca gatggtcacc gcagtttcgg ccccggcccg
     8041 aggccaagaa cagatggtcc ccagatatgg cccaaccctc agcagtttct taagacccat
     8101 cagatgtttc caggctcccc caaggacctg aaatgaccct gcgccttatt tgaattaacc
     8161 aatcagcctg cttctcgctt ctgttcgcgc gcttctgctt cccgagctct ataaaagagc
     8221 tcacaacccc tcactcggcg cgccagtcct ccgacagact gagtcgcccg ggggggatct
     8281 ggagctctcg agaattctca cgcgtcaagt ggagcaaggc aggtggacag tggatcatgg
     8341 ccttaccagt gaccgccttg ctcctgccgc tggccttgct gctccacgcc gccaggccgg
     8401 agcagaagct gatcagcgag gaggacctgc aagttcaatt ggtccagtct ggggcagaag
     8461 tgaaaaaacc gggcgcctct gtgaaagtga gctgtaaggc gtccggttat acgtttacgt
     8521 cttacgctat cagttgggtc cgccagcctc cagggcaagg tctggaatgg atgggtggcg
     8581 tcgatccaga gtacggcgga acgacatata ctcaaaaatt tcagggtaga gttaccatga
     8641 cgacggatac atctacaagt acagcgtata tggagctgtc ttcacttcgc cctgaagata
     8701 ccgctgtcta ttattgcgcg agggatagag gaatgggcaa ctactttgac tactggggtc
     8761 agggcaccct tgtgaccgtt tctagtggag gaggaggtag cgggggaggc ggttccgggg
     8821 gaggcggttc tgaattggtc atgacgcagt ctcccgctac cttgtctctg tcccctaagg
     8881 agacggcgac cctgtcctgt cgggcttcac agtctgtatc cagctatctt gcgtggtacc
     8941 agcaaaagcc agggcaggct cccaggctcc tgatttacgg agcatcaacc agggccactg
     9001 gcataccagc tcgcttttct ggtagtggtt caggcaccga cttcacattg acaattagta
     9061 gattggagcc cgaagatttc gctgtttact attgtcaaca atatagctcc tggccgttga
     9121 cgttcggcca ggggacgaaa ctggaaatca agcgcatcct ggactacagc ttcacaggtg
     9181 gcgctgggcg cgacattccc ccaccgcaga ttgaggaggc ctgtgagctg cctgagtgcc
     9241 aggtggatgc aggcaataag gtctgcaacc tgcagtgtaa taatcacgca tgtggctggg
     9301 atggtggcga ctgctccctc aacttcaatg acccctggaa gaactgcacg cagtctctac
     9361 agtgctggaa gtattttagc gacggccact gtgacagcca gtgcaactcg gccggctgcc
     9421 tctttgatgg cttcgactgc cagctcaccg agggacagtg caaccccctg tatgaccagt
     9481 actgcaagga ccacttcagt gatggccact gcgaccaggg ctgtaacagt gccgaatgtg
     9541 agtgggatgg cctagactgt gctgagcatg tacccgagcg gctggcagcc ggcaccctgg
     9601 tgctggtggt gctgcttcca cccgaccagc tacggaacaa ctccttccac tttctgcggg
     9661 agctcagcca cgtgctgcac accaacgtgg tcttcaagcg tgatgcgcaa ggccagcaga
     9721 tgatcttccc gtactatggc cacgaggaag agctgcgcaa gcacccaatc aagcgctcta
     9781 cagtgggttg ggccacctct tcactgcttc ctggtaccag tggtgggcgc cagcgcaggg
     9841 agctggaccc catggacatc cgtggctcca ttgtctacct ggagatcgac aaccggcaat
     9901 gtgtgcagtc atcctcgcag tgcttccaga gtgccaccga tgtggctgcc ttcctaggtg
     9961 ctcttgcgtc acttggcagc ctcaatattc cttacaagat tgaggccgtg aagagtgagc
    10021 cggtggagcc tccgctgccc tcgcagctgc acctcatgta cgtggcagcg gccgccttcg
    10081 tgctcctgtt ctttgtgggc tgtggggtgc tgctgtcccg caagcgccgg cggatgaagc
    10141 tgctgagcag catcgagcag gcctgtgaca tctgccggct gaagaaactg aagtgcagca
    10201 aagaaaagcc caagtgcgcc aagtgcctga agaacaactg ggagtgccgg tacagcccca
    10261 agaccaagag aagccccctg accagagccc acctgaccga ggtggaaagc cggctggaaa
    10321 gactggaaca gctgtttctg ctgatcttcc cacgcgagga cctggacatg atcctgaaga
    10381 tggacagcct gcaggacatc aaggccctgc tgaccggcct gttcgtgcag gacaacgtga
    10441 acaaggacgc cgtgaccgac agactggcca gcgtggaaac cgacatgccc ctgaccctgc
    10501 ggcagcacag aatcagcgcc accagcagca gcgaggaaag cagcaacaag ggccagcggc
    10561 agctgacagt gtctgctgct gcaggcggaa gcggaggctc tggcggatct gatgccctgg
    10621 acgacttcga cctggatatg ctgggcagcg acgccctgga tgattttgat ctggacatgc
    10681 tgggatctga cgctctggac gatttcgatc tcgacatgtt gggatcagat gcactggatg
    10741 actttgacct ggacatgctc ggatcatgag atccttgact tgcggccgca actcccacct
    10801 gcaacatgcg tgactgactg aggccgcgac tctagagtcg acctgcaggc atgcaagctt
    10861 gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
    10921 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
    10981 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
    11041 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
    11101 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
    11161 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
    11221 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
    11281 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
    11341 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
    11401 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
    11461 tccccgcatc
//
