LOCUS       sequence-49178-e         733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 733)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GUCY1B3
FEATURES             Location/Qualifiers
     source          1..733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             662..682
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             683..700
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             701..724
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cttaagaagg agatatacta tgcacaagcg tccggttccg gccaaacgct atgacaacgt
       61 gactattctg ttctccggta tcgtagggtt taatgcattt tgtagcaaac atgcgtcggg
      121 cgagggtgct atgaagattg ttaacctgct gaatgatctg tacacacgtt tcgatacgct
      181 gaccgactct cgcaaaaacc cgtttgtgta taaagtcgaa actgtttgcg ataagtatat
      241 gacagtgagt ggtctgccgg agccgtgcat ccatcacgcc cgttcaattt gtcatctggc
      301 actggacatg atggaaattg cggggcaggt acaggtcgat ggcgaaagcg ttcaaatcac
      361 gattggtatc cacaccgggg aggtggtaac tggcgtcatt ggtcagcgca tgccgcgtta
      421 ctccctgttc ggcaatacgg ttaacctgac ctcccgcaca gaaaccactg gtgaaaaagg
      481 gaaaatcaat gtgtcggagt atacataccg ttgtctgatg tctccggaaa acagtgaccc
      541 gcaatttcat ctggagcacc gcggcccggt aagcatgaag ggtaaaaaag aaccgatgca
      601 ggtctggttc ctgtcacgta agaatacggg caccgaagag actaaacagg atgatgacgc
      661 agagaacctc tacttccaat cgcaccatca tcaccaccat gattacaagg atgacgacga
      721 taagtgagga tcc
//
